Paper · 2026 · International Journal of Phytoremediation

Mitigating stress and boosting metabolites through the impact of ZnO nanoparticles on Stevia rebaudiana and Ocimum basilicum

Ayza Ali; Zainab Zia; Muhammad Waqas Kazmi; et al.

5
claims
8
verbatim quotes
1
citations
2
research areas
—
Not open access

Abstract

Plant growth is usually compromised by abiotic and biotic stressors that decrease germination, yield, and synthesis of useful metabolites. Zinc oxide nanoparticles (ZnO-NPs) have exhibited potential in increasing plant tolerance and metabolic yield. This research assesses the impact of ZnO-NPs on Stevia rebaudiana and Ocimum basilicum in a randomized pot experiment with seed priming at concentrations of 0–250 mg L−1. The nanoparticles were identified through UV-vis spectroscopy (maximum absorption at 380 nm), FTIR, and XRD, proving their crystalline hexagonal wurtzite nature and functional groups (C = O, C-O, O-H). Germination in O. basilicum was highest at 50 mg L−1, with maximum shoot and root growth at 100 mg L−1, whereas Stevia showed a positive response only at 50 mg L−1. GC-MS analysis revealed remarkable enhancement of fatty acids, phenols, and terpenes in ZnO-NP treatment. These observations imply ZnO-NPs have the potential to promote plant growth and secondary metabolite production, yet species- and dose-dependent effects need to be considered with care.

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Claims 5

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Ocimum basilicum germination was highest at 50 mg L−1.

Taxon
O. basilicumOcimum basilicume0
Treatment
50 mg L−1e0
Outcome
Germinatione0
Direction
higheste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ocimum basilicum
Ocimum basilicum · TaxonCastielli · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Germination in O. basilicum was highest at 50 mg L−1”

✓ located verbatim in the source · abstract, characters 623–675 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Ocimum basilicum shoot growth was maximum at 100 mg L−1.

Taxon
Ocimum basilicumOcimum basilicumt0
Organ / stage
shoote0
Treatment
100 mg L−1e0
Outcome
growthe0
Direction
maximume0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ocimum basilicum
Ocimum basilicum · TaxonCastielli · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“with maximum shoot and root growth at 100 mg L−1”

✓ located verbatim in the source · abstract, characters 677–725 · show in text ↑
t0

“Ocimum basilicum”

✓ located verbatim in the source · title, characters 108–124 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Ocimum basilicum root growth was maximum at 100 mg L−1.

Taxon
Ocimum basilicumOcimum basilicumt0
Treatment
100 mg L−1e0
Outcome
root growthe0
Direction
maximume0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ocimum basilicum
Ocimum basilicum · TaxonCastielli · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“with maximum shoot and root growth at 100 mg L−1”

✓ located verbatim in the source · abstract, characters 677–725 · show in text ↑
t0

“Ocimum basilicum”

✓ located verbatim in the source · title, characters 108–124 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Stevia responded positively only at 50 mg L−1.

Taxon
SteviaSteviae0
Treatment
50 mg L−1e0
Outcome
positive responsee0
Direction
positivee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Stevia
Stevia · Taxon · picture of the genus (one of its species)Ethel Aardvark · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e0

“whereas Stevia showed a positive response only at 50 mg L−1”

✓ located verbatim in the source · abstract, characters 727–786 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

ZnO nanoparticle treatment enhanced fatty acids, phenols and terpenes.

Taxon
Stevia rebaudiana and Ocimum basilicumOcimum basilicumStevia rebaudianat0
Treatment
ZnO-NP treatmente0
Outcome
fatty acids, phenols, and terpenese0
Direction
enhancemente0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ocimum basilicum
Ocimum basilicum · TaxonCastielli · CC BY-SA 3.0 · Wikimedia Commons ↗
Stevia rebaudiana
Stevia rebaudiana · TaxonEthel Aardvark · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e0

“GC-MS analysis revealed remarkable enhancement of fatty acids, phenols, and terpenes in ZnO-NP treatment”

✓ located verbatim in the source · abstract, characters 788–892 · show in text ↑
t0

“Stevia rebaudiana and Ocimum basilicum”

✓ located verbatim in the source · title, characters 86–124 · show in text ↑

zinc oxide · quality: oil · Ocimum basilicum (basil)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

nanoparticle · quality: oil · Ocimum basilicum (basil)Positive and negative results

9 papers: 8 positive, 1 negative or no effect

7 papers in the same direction as this paper · 1 in the opposite direction

  1. no effect
  2. positive
  3. positive

5 papers more in this group

See all 9 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.