Paper · 2026 · Fermentation

A Comparative Study of Cutting Height and Fermentation Method on Cenchrus fungigraminus Silage: Effects of Natural Fermentation Versus Microbial Inoculant on Silage Quality and Fiber Degradation

Hongyuan He; Ziting Wang; Fuke Hako; et al.

13
claims
33
verbatim quotes
0
citations
2
research areas
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Open access · cc-by

Abstract

Cenchrus fungigraminus (Juncao) is a high-yielding, fast-growing forage crop with considerable potential for livestock feed; however, optimizing its processing is essential for cost reduction and quality enhancement. This study comprised three components: (1) a comprehensive analysis of 25 on-farm silage samples from five locations in Southwest China using Grey Relational Analysis (GRA); (2) an assessment of the effects of three cutting heights (low: 100–150 cm; mid: 150–200 cm; high: 200–250 cm) on silage quality; and (3) a comparison of silage quality between natural fermentation and microbial inoculant treatments using mature Juncao (250–300 cm). The results showed that: (1) in the on-farm silage samples, carbon supplementation was significantly positively correlated with total digestible nutrients (TDN), relative feed value (RFV), ether extract (EE), and sensory evaluation (p < 0.05), and the GRA identified the top-ranked treatments, including J2, J3, J6, X6, and J5; (2) in the cutting height trials, fiber content increased significantly with cutting height (p < 0.05), while crude protein (CP) and TDN decreased significantly (p < 0.05). The 200–250 cm group exhibited optimal fermentation quality, characterized by the highest total volatile fatty acids (total VFA) and lactic acid concentrations, alongside the lowest pH and ammonia nitrogen/total nitrogen ratios (NH3-N/TN); (3) in the inoculant comparison, the natural fermentation group demonstrated significantly higher degradation rates of acid detergent fiber (ADF), neutral detergent fiber (NDF), and acid detergent lignin (ADL) compared to the microbial inoculant group, while also maintaining a lower pH, higher total VFA and lactic acid. Consequently, for on-farm production, carbon supplementation is recommended to improve silage quality. Although cutting Juncao below 200 cm provides higher nutritional value, a height of 200–250 cm is advised to ensure optimal fermentation characteristics. Furthermore, natural fermentation proves superior to microbial inoculant treatment for mature Juncao. Together, these measures offer an effective strategy for producing high-quality Juncao silage.

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Claims 13

Associationobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

In on-farm Juncao silage samples, carbon supplementation was significantly positively correlated with total digestible nutrients, relative feed value and ether extract.

Taxon
Cenchrus fungigraminusCenchrus fungigraminust0
Treatment
carbon supplementatione0
Outcome
total digestible nutrients (TDN), relative feed value (RFV), ether extract (EE)e0
Direction
positively correlatede0

Verbatim

t0

“Cenchrus fungigraminus Silage”

✓ located verbatim in the source · title, characters 65–94 · show in text ↑
e0

“carbon supplementation was significantly positively correlated with total digestible nutrients (TDN), relative feed value (RFV), ether extract (EE), and sensory evaluation (p < 0.05)”

✓ located verbatim in the source · abstract, characters 718–900 · show in text ↑
Associationobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

In on-farm Juncao silage samples, carbon supplementation was significantly positively correlated with sensory evaluation.

Taxon
Cenchrus fungigraminusCenchrus fungigraminust0
Treatment
carbon supplementatione0
Outcome
sensory evaluatione0
Direction
positively correlatede0

Verbatim

t0

“Cenchrus fungigraminus Silage”

✓ located verbatim in the source · title, characters 65–94 · show in text ↑
e0

“carbon supplementation was significantly positively correlated with total digestible nutrients (TDN), relative feed value (RFV), ether extract (EE), and sensory evaluation (p < 0.05)”

✓ located verbatim in the source · abstract, characters 718–900 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Grey Relational Analysis of the on-farm Juncao silage samples identified J2, J3, J6, X6 and J5 among the top-ranked treatments.

Taxon
Cenchrus fungigraminusCenchrus fungigraminust0
Outcome
top-ranked treatmentse0
Value
J2, J3, J6, X6, and J5e0

Verbatim

t0

“Cenchrus fungigraminus Silage”

✓ located verbatim in the source · title, characters 65–94 · show in text ↑
e0

“the GRA identified the top-ranked treatments, including J2, J3, J6, X6, and J5”

✓ located verbatim in the source · abstract, characters 906–984 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Fiber content of Juncao silage increased significantly with cutting height.

Taxon
Cenchrus fungigraminusCenchrus fungigraminust0
Treatment
cutting heighte0
Outcome
fiber contente0
Direction
increasede0

Not reportedComparator

Verbatim

t0

“Cenchrus fungigraminus Silage”

✓ located verbatim in the source · title, characters 65–94 · show in text ↑
e0

“fiber content increased significantly with cutting height”

✓ located verbatim in the source · abstract, characters 1020–1077 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Crude protein and total digestible nutrients of Juncao silage decreased significantly with cutting height.

Taxon
Cenchrus fungigraminusCenchrus fungigraminust0
Treatment
cutting heighte1
Outcome
crude protein (CP) and TDNe0
Direction
decreasede0

Not reportedComparator

Verbatim

t0

“Cenchrus fungigraminus Silage”

✓ located verbatim in the source · title, characters 65–94 · show in text ↑
e0

“crude protein (CP) and TDN decreased significantly (p < 0.05)”

✓ located verbatim in the source · abstract, characters 1096–1157 · show in text ↑
e1

“cutting height trials”

✓ located verbatim in the source · abstract, characters 997–1018 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Juncao cut at 200-250 cm gave the highest total volatile fatty acids and lactic acid concentrations in silage.

Taxon
Cenchrus fungigraminusCenchrus fungigraminust0
Treatment
200–250 cme0
Outcome
total volatile fatty acids (total VFA) and lactic acid concentrationse1
Direction
higheste1

Not reportedComparator

Verbatim

t0

“Cenchrus fungigraminus Silage”

✓ located verbatim in the source · title, characters 65–94 · show in text ↑
e0

“The 200–250 cm group exhibited optimal fermentation quality”

✓ located verbatim in the source · abstract, characters 1159–1218 · show in text ↑
e1

“the highest total volatile fatty acids (total VFA) and lactic acid concentrations”

✓ located verbatim in the source · abstract, characters 1237–1318 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Juncao cut at 200-250 cm gave the lowest pH and ammonia nitrogen to total nitrogen ratio in silage.

Taxon
Cenchrus fungigraminusCenchrus fungigraminust0
Treatment
200–250 cme0
Outcome
pH and ammonia nitrogen/total nitrogen ratios (NH3-N/TN)e1
Direction
loweste1

Not reportedComparator

Verbatim

t0

“Cenchrus fungigraminus Silage”

✓ located verbatim in the source · title, characters 65–94 · show in text ↑
e0

“The 200–250 cm group exhibited optimal fermentation quality”

✓ located verbatim in the source · abstract, characters 1159–1218 · show in text ↑
e1

“the lowest pH and ammonia nitrogen/total nitrogen ratios (NH3-N/TN)”

✓ located verbatim in the source · abstract, characters 1330–1397 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Mature Juncao silage under natural fermentation showed significantly higher degradation rates of ADF, NDF and ADL than with microbial inoculant.

Taxon
Cenchrus fungigraminusCenchrus fungigraminust0
Treatment
natural fermentatione0
Outcome
degradation rates of acid detergent fiber (ADF), neutral detergent fiber (NDF), and acid detergent lignin (ADL)e0
Direction
highere0
Comparator
microbial inoculant groupe0

Verbatim

t0

“Cenchrus fungigraminus Silage”

✓ located verbatim in the source · title, characters 65–94 · show in text ↑
e0

“the natural fermentation group demonstrated significantly higher degradation rates of acid detergent fiber (ADF), neutral detergent fiber (NDF), and acid detergent lignin (ADL) compared to the microbial inoculant group”

✓ located verbatim in the source · abstract, characters 1432–1650 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Natural fermentation of mature Juncao silage maintained a lower pH than microbial inoculant treatment.

Taxon
Cenchrus fungigraminusCenchrus fungigraminust0
Treatment
natural fermentatione0
Outcome
pHe1
Direction
lowere1
Comparator
microbial inoculant groupe2

Verbatim

t0

“Cenchrus fungigraminus Silage”

✓ located verbatim in the source · title, characters 65–94 · show in text ↑
e0

“the natural fermentation group”

✓ located verbatim in the source · abstract, characters 1432–1462 · show in text ↑
e1

“while also maintaining a lower pH”

✓ located verbatim in the source · abstract, characters 1652–1685 · show in text ↑
e2

“microbial inoculant group”

✓ located verbatim in the source · abstract, characters 1625–1650 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Natural fermentation of mature Juncao silage gave higher total VFA and lactic acid than microbial inoculant treatment.

Taxon
Cenchrus fungigraminusCenchrus fungigraminust0
Treatment
natural fermentatione0
Outcome
total VFA and lactic acide1
Direction
highere1
Comparator
microbial inoculant groupe2

Verbatim

t0

“Cenchrus fungigraminus Silage”

✓ located verbatim in the source · title, characters 65–94 · show in text ↑
e0

“the natural fermentation group”

✓ located verbatim in the source · abstract, characters 1432–1462 · show in text ↑
e1

“higher total VFA and lactic acid”

✓ located verbatim in the source · abstract, characters 1687–1719 · show in text ↑
e2

“microbial inoculant group”

✓ located verbatim in the source · abstract, characters 1625–1650 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Cutting Juncao below 200 cm provides higher nutritional value.

Taxon
Juncaoe0
Treatment
cutting Juncao below 200 cme0
Outcome
nutritional valuee0
Direction
highere0

Not reportedComparator

Verbatim

t0

“Cenchrus fungigraminus Silage”

✓ located verbatim in the source · title, characters 65–94 · show in text ↑
e0

“cutting Juncao below 200 cm provides higher nutritional value”

✓ located verbatim in the source · abstract, characters 1833–1894 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

For on-farm production, carbon supplementation is recommended to improve silage quality.

Taxon
Cenchrus fungigraminusCenchrus fungigraminust0
Outcome
carbon supplementation is recommended to improve silage qualitye0

Verbatim

t0

“Cenchrus fungigraminus Silage”

✓ located verbatim in the source · title, characters 65–94 · show in text ↑
e0

“for on-farm production, carbon supplementation is recommended to improve silage quality”

✓ located verbatim in the source · abstract, characters 1735–1822 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

A cutting height of 200-250 cm is advised for Juncao silage to ensure optimal fermentation characteristics.

Taxon
Cenchrus fungigraminusCenchrus fungigraminust0
Outcome
a height of 200–250 cm is advised to ensure optimal fermentation characteristicse0

Verbatim

t0

“Cenchrus fungigraminus Silage”

✓ located verbatim in the source · title, characters 65–94 · show in text ↑
e0

“a height of 200–250 cm is advised to ensure optimal fermentation characteristics”

✓ located verbatim in the source · abstract, characters 1896–1976 · show in text ↑