Abstract
Plant cells can undergo cellular reprogramming, enabling pluripotent callus formation from excised leaves. Despite this pluripotency, organs regenerated from leaf callus have predominantly been confined to conventional shoots and roots, leaving the potential to regenerate other specialized organs unknown. In this study, we identified that stolons can be regenerated from potato leaf callus. Furthermore, we demonstrate that Agrobacterium tumefaciens stimulation efficiently induces stolon regeneration and subsequent tuber development from potato leaf callus. The induction of stolon regeneration is abolished when biological activity is removed from the bacterial cultures, indicating that viable bacterial cells are required for this process. Comparative assays using various strains reveal that the C58 chromosomal background is essential for this enhancement. Integrating transcriptome analysis with transgenic functional validation, we find that phosphatidylethanolamine-binding protein (PEBP) family genes are closely involved in this response. Furthermore, we observe that viruses do not readily spread to regenerated stolons through the callus, which lacks a continuous vascular system to serve as a pathway for virus movement. Our findings demonstrate that bacterial stimulation triggers stolon regeneration from pluripotent leaf callus, offering a potential approach for the production of virus-free storage organs in potato.
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Claims 6
Claim · model-written summary
Stolons can be regenerated from potato leaf callus.
- Taxon
- potato
Solanum tuberosume0 - Organ / stage
- leaf calluse0
- Outcome
- stolons can be regeneratede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified that stolons can be regenerated from potato leaf callus”
Claim · model-written summary
Agrobacterium tumefaciens stimulation efficiently induced stolon regeneration and subsequent tuber development from potato leaf callus.
- Taxon
- potatoSolanum tuberosume0
- Organ / stage
- leaf calluse0
- Treatment
- Agrobacterium tumefaciens stimulatione0
- Interactor
- Agrobacterium tumefaciens
Agrobacterium tumefaciense0other - Outcome
- stolon regeneration and subsequent tuber developmente0
- Direction
- inducese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Agrobacterium tumefaciens stimulation efficiently induces stolon regeneration and subsequent tuber development from potato leaf callus”
Claim · model-written summary
The induction of stolon regeneration from potato leaf callus was abolished when biological activity was removed from the bacterial cultures, so viable cells are required.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- biological activity is removed from the bacterial culturese0
- Interactor
- bacterial culturese0other
- Outcome
- induction of stolon regeneratione0
- Direction
- abolishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potato leaf callus”
e0“The induction of stolon regeneration is abolished when biological activity is removed from the bacterial cultures”
Claim · model-written summary
Comparative assays with various strains showed that the C58 chromosomal background is essential for the enhancement of stolon regeneration.
- Taxon
- potatoSolanum tuberosumt0
- Outcome
- enhancemente0
- Qualifier
- the C58 chromosomal background is essentiale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potato leaf callus”
e0“Comparative assays using various strains reveal that the C58 chromosomal background is essential for this enhancement”
Claim · model-written summary
Transcriptome analysis with transgenic functional validation showed that PEBP family genes are closely involved in the Agrobacterium-induced stolon regeneration response.
- Taxon
- potatoSolanum tuberosumt0
- Gene
- phosphatidylethanolamine-binding protein (PEBP) family genese0
- Outcome
- this responsee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potato leaf callus”
e0“Integrating transcriptome analysis with transgenic functional validation, we find that phosphatidylethanolamine-binding protein (PEBP) family genes are closely involved in this response”
Claim · model-written summary
Viruses did not readily spread to regenerated stolons through the callus, which lacks a continuous vascular system for virus movement.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- regenerated stolonse0
- Outcome
- spread to regenerated stolonse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potato leaf callus”
e0“viruses do not readily spread to regenerated stolons through the callus, which lacks a continuous vascular system to serve as a pathway for virus movement”