Paper · 2026 · Frontiers in Nutrition

Wheat aroma biomarkers induced by saline-alkali soil: based on HS-SPME-GC-MS and molecular docking

Jiarong Zhang; Hao Wang; Zihe Wang; et al.

7
claims
7
verbatim quotes
0
citations
2
research areas
✓
Open access · cc-by

Abstract

Background The precise volatile compounds accountable for the characteristic scent of the wheat aroma were presently unidentified. Given the prominent manifestation of “wheat aroma” in wheat cultivated in saline-alkali (SA) soil, five wheat varieties planted in saline-alkali soil and control soil (low saline-alkali soil) to create “rich-aroma” and “bland-aroma” samples, respectively. Methods The volatile profiles of these two groups of samples were analyzed using headspace solid-phase microextraction coupled with gas chromatographyed with gas chromathes-SPME-GC-MS), and differential characteristic volatile compounds between “rich-aroma” and “bland-aroma” samples were identified by combining paired t -tests, relative odor activity values (ROAVs), and molecular docking analysis. Results A total of 75 volatile compounds were identified using HS-SPME-GC-MS. Paired t -test result revealed that a significant increase ( p < 0.05) in the peak areas and relative contents of limonene (>109.74% increase), β-pinene (> 474.09% increase) contrasted with reduced butanoic acid (>38.71% reduction) and (E,E)-3,5-octadien-2-one (>16.05% reduction) in saline-alkali soil-cultivated wheats. Molecular docking demonstrated high binding energies (<-4.25 kcal/mol) of limonene, β-pinene, and (E,E)-3,5-octadien-2-one to olfactory receptors, corroborated by relative odor activity values (ROAVs. > 1). Conclusion Limonene, β-pinene and (E,E)-3,5-octadien-2-one are identified as possible saline-alkali soil-induced wheat aroma biomarkers, and offer theoretical insights for verificating wheat aroma. Identifying potential characteristic biomarkers in wheat under saline-alkali stress provides important theoretical insights for improving the flavor characteristics of wheat.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 7

Observationobservedoriginal resultmeasured

Claim · model-written summary

Seventy-five wheat volatile compounds were identified.

Outcome
volatile compoundse0
Value
75e0

Not reportedTaxon

Verbatim

e0

“A total of 75 volatile compounds were identified using HS-SPME-GC-MS.”

✓ located verbatim in the source · abstract, characters 796–865 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Limonene increased by over 109.74% in saline-alkali-grown wheat.

Taxon
wheatsTriticume0
Treatment
saline-alkali soile0
Outcome
limonenee0
Direction
increasee0
Value
>109.74%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum
Triticum · Taxon · picture from its Wikimedia Commons categoryMottaW · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“a significant increase ( p < 0.05) in the peak areas and relative contents of limonene (>109.74% increase), β-pinene (> 474.09% increase) contrasted with reduced butanoic acid (>38.71% reduction) and (E,E)-3,5-octadien-2-one (>16.05% reduction) in saline-alkali soil-cultivated wheats.”

✓ located verbatim in the source · abstract, characters 902–1187 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

β-pinene increased by over 474.09% in saline-alkali-grown wheat.

Taxon
wheatsTriticume0
Treatment
saline-alkali soile0
Outcome
β-pinenee0
Direction
increasee0
Value
> 474.09%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum
Triticum · Taxon · picture from its Wikimedia Commons categoryMottaW · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“a significant increase ( p < 0.05) in the peak areas and relative contents of limonene (>109.74% increase), β-pinene (> 474.09% increase) contrasted with reduced butanoic acid (>38.71% reduction) and (E,E)-3,5-octadien-2-one (>16.05% reduction) in saline-alkali soil-cultivated wheats.”

✓ located verbatim in the source · abstract, characters 902–1187 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Butanoic acid decreased by over 38.71% in saline-alkali-grown wheat.

Taxon
wheatsTriticume0
Treatment
saline-alkali soile0
Outcome
butanoic acide0
Direction
reducede0
Value
>38.71%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum
Triticum · Taxon · picture from its Wikimedia Commons categoryMottaW · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“a significant increase ( p < 0.05) in the peak areas and relative contents of limonene (>109.74% increase), β-pinene (> 474.09% increase) contrasted with reduced butanoic acid (>38.71% reduction) and (E,E)-3,5-octadien-2-one (>16.05% reduction) in saline-alkali soil-cultivated wheats.”

✓ located verbatim in the source · abstract, characters 902–1187 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

(E,E)-3,5-octadien-2-one decreased by over 16.05% in saline-alkali-grown wheat.

Taxon
wheatsTriticume0
Treatment
saline-alkali soile0
Outcome
(E,E)-3,5-octadien-2-onee0
Direction
reducede0
Value
>16.05%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum
Triticum · Taxon · picture from its Wikimedia Commons categoryMottaW · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“a significant increase ( p < 0.05) in the peak areas and relative contents of limonene (>109.74% increase), β-pinene (> 474.09% increase) contrasted with reduced butanoic acid (>38.71% reduction) and (E,E)-3,5-octadien-2-one (>16.05% reduction) in saline-alkali soil-cultivated wheats.”

✓ located verbatim in the source · abstract, characters 902–1187 · show in text ↑
Observationobservedoriginal resultsimulated

Claim · model-written summary

Docking found high olfactory-receptor binding energies for limonene, β-pinene and (E,E)-3,5-octadien-2-one.

Outcome
high binding energiese0
Value
<-4.25 kcal/mole0
Qualifier
olfactory receptorse0

Not reportedTaxon

Verbatim

e0

“Molecular docking demonstrated high binding energies (<-4.25 kcal/mol) of limonene, β-pinene, and (E,E)-3,5-octadien-2-one to olfactory receptors, corroborated by relative odor activity values (ROAVs. > 1).”

✓ located verbatim in the source · abstract, characters 1188–1394 · show in text ↑
Observationauthor interpretationoriginal resultmeasured

Claim · model-written summary

Limonene, β-pinene and (E,E)-3,5-octadien-2-one were proposed as saline-alkali-induced wheat aroma biomarkers.

Outcome
possible saline-alkali soil-induced wheat aroma biomarkerse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Limonene, β-pinene and (E,E)-3,5-octadien-2-one are identified as possible saline-alkali soil-induced wheat aroma biomarkers”

✓ located verbatim in the source · abstract, characters 1406–1530 · show in text ↑

salinity · molecular: metabolites · Triticum (wheats)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. negative
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.