Abstract
BACKGROUND/OBJECTIVES: Nitrogen, as an indispensable macroelement for plants, is essential for tuber development. The objective of the present study was to ascertain the key factors underlying nitrogen regulation of potato tuber formation. METHODS: The potato variety Kexin 37 was used as the material, and nitrogen deficiency, normal nitrogen level and excessive nitrogen level were employed as treatments, respectively. The response of potato tuber formation to nitrogen was systematically analyzed from the perspective of physiology and transcriptomics. RESULTS: Nitrogen deficiency led to the thickening of the cell wall and plasma membrane, an increase in intercellular space and a decrease in mitochondria in the stolon. The plant height, chlorophyll content, dry matter quality and nitrogen accumulation were significantly reduced, and the number of tubers per plant, tuber weight per plant and commodity rate were significantly reduced. Excessive nitrogen application resulted in late maturity of plants and excessive formation of small potatoes. Transcriptome analysis revealed that differentially expressed genes related to nitrogen stress were mainly enriched in pathways associated with material transport, cell division and carbohydrate metabolism. In addition, there are a series of hub genes in response to nitrogen stress, including polyubiquitin-like, auxin response factor 7-like and protein RRP6-like 2. By constructing a co-expression network, transcription factors (TFs) such as C2H2, WRKY and ARF are involved in regulating tuber formation. CONCLUSIONS: The present study constitutes an investigation into the identification of hub genes and potential pathways associated with the formation of potato tubers under varying nitrogen conditions. It provides new insights for further study on enhancing nitrogen use efficiency in potato.
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Claims 7
Claim · model-written summary
Nitrogen deficiency thickened cell walls and plasma membranes, increased intercellular space, and decreased mitochondria in stolons.
- Taxon
- potato
Solanum tuberosume1 - Organ / stage
- stolone0
- Treatment
- Nitrogen deficiencye0
- Outcome
- cell wall and plasma membranee0
- Direction
- thickeninge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nitrogen deficiency led to the thickening of the cell wall and plasma membrane, an increase in intercellular space and a decrease in mitochondria in the stolon”
e1“The potato variety Kexin 37”
Claim · model-written summary
Nitrogen deficiency significantly reduced plant height, chlorophyll content, dry matter quality, and nitrogen accumulation.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- nitrogen deficiencye2
- Outcome
- plant height, chlorophyll content, dry matter quality and nitrogen accumulatione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The plant height, chlorophyll content, dry matter quality and nitrogen accumulation were significantly reduced”
e1“The potato variety Kexin 37”
e2“Nitrogen deficiency”
Claim · model-written summary
Nitrogen deficiency significantly reduced tuber number, tuber weight and commodity rate.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- nitrogen deficiencye2
- Outcome
- number of tubers per plant, tuber weight per plant and commodity ratee0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the number of tubers per plant, tuber weight per plant and commodity rate were significantly reduced”
e1“The potato variety Kexin 37”
e2“Nitrogen deficiency”
Claim · model-written summary
Excess nitrogen delayed maturity and caused excessive small potato formation.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- Excessive nitrogen applicatione0
- Outcome
- late maturity of plants and excessive formation of small potatoese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Excessive nitrogen application resulted in late maturity of plants and excessive formation of small potatoes”
e1“The potato variety Kexin 37”
Claim · model-written summary
Nitrogen-stress-responsive genes were enriched in transport, cell division, and carbohydrate metabolism pathways.
- Taxon
- potatoSolanum tuberosume1
- Outcome
- differentially expressed genes related to nitrogen stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“differentially expressed genes related to nitrogen stress were mainly enriched in pathways associated with material transport, cell division and carbohydrate metabolism”
e1“The potato variety Kexin 37”
Claim · model-written summary
Hub genes responding to nitrogen stress included polyubiquitin-like, auxin response factor 7-like, and protein RRP6-like 2.
- Taxon
- potatoSolanum tuberosume1
- Gene
- polyubiquitin-like, auxin response factor 7-like and protein RRP6-like 2e0
- Outcome
- hub genes in response to nitrogen stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“hub genes in response to nitrogen stress, including polyubiquitin-like, auxin response factor 7-like and protein RRP6-like 2”
e1“The potato variety Kexin 37”
Claim · model-written summary
Co-expression analysis implicated C2H2, WRKY and ARF transcription factors in tuber formation.
- Taxon
- potatoSolanum tuberosume1
- Gene
- C2H2, WRKY and ARFe0
- Outcome
- tuber formatione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcription factors (TFs) such as C2H2, WRKY and ARF are involved in regulating tuber formation”
e1“The potato variety Kexin 37”
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