Abstract
Cytokinin is a plant hormone that regulates several yield-related traits in plants. Previously, it was demonstrated that in tetraploid oilseed rape ( Brassica napus L.), mutation of all four cytokinin-degrading BnCKX3 and both BnCKX5 genes resulted in increased cytokinin concentration, larger and more active inflorescence meristems, and a higher number of ovules per gynoecium. This resulted in the formation of more flowers and pods on the main stem, thereby increasing seed yield from the main stem of the plants. Here, we investigated the relative contributions of distinct combinations of BnCKX3 and BnCKX5 genes of the A and C genomes to these yield components. Our analysis revealed an unexpectedly strong role for BnCKX5 in regulating these traits and identified distinct supportive BnCKX3 gene mutant combinations. These findings facilitate the selection of relevant alleles for breeding. Furthermore, seeds from BnCKX gene mutant plants showed oil content and concentrations of unsaturated fatty acids similar to those of the wild type. Taken together, this study provides further insight into the role of cytokinin and BnCKX genes in regulating yield components in oilseed rape and provides novel information on functionally relevant alleles.
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Claims 5
Claim · model-written summary
Earlier work showed that mutating all four BnCKX3 and both BnCKX5 genes raised cytokinin concentration, enlarged and activated inflorescence meristems and increased ovules per gynoecium.
- Taxon
- Brassica napus L.
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napust0 - Treatment
- mutation of all four cytokinin-degrading BnCKX3 and both BnCKX5 genese0
- Gene
- BnCKX3 and both BnCKX5 genese0
- Outcome
- cytokinin concentration, larger and more active inflorescence meristems, and a higher number of ovules per gynoeciume0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
t0“Brassica napus L.”
e0“mutation of all four cytokinin-degrading BnCKX3 and both BnCKX5 genes resulted in increased cytokinin concentration, larger and more active inflorescence meristems, and a higher number of ovules per gynoecium”
Claim · model-written summary
The analysis revealed an unexpectedly strong role for BnCKX5 in regulating the yield-component traits.
- Taxon
- Brassica napus L.Brassica napust0
- Gene
- BnCKX5e0
- Outcome
- these traitse0
- Qualifier
- unexpectedly strong rolee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica napus L.”
e0“Our analysis revealed an unexpectedly strong role for BnCKX5 in regulating these traits”
Claim · model-written summary
Distinct supportive BnCKX3 gene mutant combinations were identified.
- Taxon
- Brassica napus L.Brassica napust0
- Gene
- BnCKX3e0
- Outcome
- distinct supportive BnCKX3 gene mutant combinationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica napus L.”
e0“identified distinct supportive BnCKX3 gene mutant combinations”
Claim · model-written summary
Seeds from BnCKX gene mutant plants had oil content and unsaturated fatty acid concentrations similar to the wild type.
- Taxon
- Brassica napus L.Brassica napust0
- Organ / stage
- seedse0
- Treatment
- BnCKX gene mutant plantse0
- Gene
- BnCKXe0
- Outcome
- oil content and concentrations of unsaturated fatty acidse0
- Direction
- similare0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica napus L.”
e0“seeds from BnCKX gene mutant plants showed oil content and concentrations of unsaturated fatty acids similar to those of the wild type”
Claim · model-written summary
The findings facilitate selection of relevant alleles for breeding.
- Taxon
- Brassica napus L.Brassica napust0
- Outcome
- selection of relevant alleles for breedinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica napus L.”
e0“These findings facilitate the selection of relevant alleles for breeding”