Abstract
Micropropagation is instrumental for the rapid multiplication of elite cassava varieties with improved traits. However, it is often impaired by transplanting stress associated with acclimatization, which occurs during the acclimatization, transfer from in vitro to soil condition. Arbuscular Mycorrhizal Fungi (AMF) could be used to reduce transplant shock symptoms through improved nutrient acquisition and physiological function. In this study, we investigated the impact of inoculating anAMF, R. irregularis , on the growth of two varieties of micropropagated cassava on plant physiological traits, the δ 13 C and δ 15 N isotopic signatures, and nitrogen use efficiency (NUE) during acclimatisation. In the early acclimatization stage, (4–8 weeks after transplanting,WAT), AMF inoculation increased height, stem diameter, and leaf number by 61%, 50%, and 57% respectively, compared to non-inoculated plants in non-sterile soil. Under subsequent water deficit at 8–10 WAT, inoculated plants were better preserved regarding height and stem thickness. After 10 weeks, AMF root colonization increased by 57% and 61% under water-deficit and well-watered conditions respectively, compared to non-inoculated plants. Total biomass, C:N and NUE increased significantly in inoculated plantlets under well-watered conditions, with lowered δ 15 N and N concentrations due to N dilution from a 63% biomass increase. In water-deficient conditions, regardless of inoculation, δ 13 C decreased, suggesting the dominant role of water availability in carbon assimilation. Overall, we found important benefits of AMF inoculation. Yet, to fully harness the benefits of AMF for micropropagated cassava, proper nitrogen management is essential, as the enhanced growth conferred by increased root colonization rate could lead to N deficiency.
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Claims 10
Claim · model-written summary
AMF inoculation increased cassava plant height during early acclimatisation by 61%.
Verbatim
e0“AMF inoculation increased height, stem diameter, and leaf number by 61%, 50%, and 57% respectively, compared to non-inoculated plants in non-sterile soil”
e5“the growth of two varieties of micropropagated cassava”
e6“inoculating anAMF, R. irregularis”
Claim · model-written summary
AMF inoculation increased cassava stem diameter during early acclimatisation by 50%.
Verbatim
e0“AMF inoculation increased height, stem diameter, and leaf number by 61%, 50%, and 57% respectively, compared to non-inoculated plants in non-sterile soil”
e5“the growth of two varieties of micropropagated cassava”
e6“inoculating anAMF, R. irregularis”
Claim · model-written summary
AMF inoculation increased cassava leaf number during early acclimatisation by 57%.
Verbatim
e0“AMF inoculation increased height, stem diameter, and leaf number by 61%, 50%, and 57% respectively, compared to non-inoculated plants in non-sterile soil”
e5“the growth of two varieties of micropropagated cassava”
e6“inoculating anAMF, R. irregularis”
Claim · model-written summary
AMF root colonization increased by 57% under water-deficit conditions compared with non-inoculated plants.
Verbatim
e1“After 10 weeks, AMF root colonization increased by 57% and 61% under water-deficit and well-watered conditions respectively, compared to non-inoculated plants”
e5“the growth of two varieties of micropropagated cassava”
e6“inoculating anAMF, R. irregularis”
Claim · model-written summary
AMF root colonization increased by 61% under well-watered conditions compared with non-inoculated plants.
Verbatim
e1“After 10 weeks, AMF root colonization increased by 57% and 61% under water-deficit and well-watered conditions respectively, compared to non-inoculated plants”
e5“the growth of two varieties of micropropagated cassava”
e6“inoculating anAMF, R. irregularis”
Claim · model-written summary
Total biomass, C:N and NUE increased significantly in inoculated cassava plantlets under well-watered conditions.
- Taxon
- micropropagated cassavae5
- Treatment
- inoculated plantletse2
- Interactor
- R. irregularise6symbiont
- Outcome
- Total biomass, C:N and NUEe2
- Direction
- increasede2
- Setting
- well-watered conditionse2
Not reportedComparator
Verbatim
e2“Total biomass, C:N and NUE increased significantly in inoculated plantlets under well-watered conditions”
e5“the growth of two varieties of micropropagated cassava”
e6“inoculating anAMF, R. irregularis”
Claim · model-written summary
Foliar δ 15 N and N concentrations were lowered in AMF-inoculated cassava plantlets under well-watered conditions.
- Taxon
- micropropagated cassavae5
- Treatment
- inoculating anAMFe6
- Interactor
- R. irregularise6symbiont
- Outcome
- δ 15 N and N concentrationse3
- Direction
- lowerede3
Not reportedComparator
Verbatim
e3“with lowered δ 15 N and N concentrations due to N dilution from a 63% biomass increase”
e5“the growth of two varieties of micropropagated cassava”
e6“inoculating anAMF, R. irregularis”
Claim · model-written summary
Under water-deficient conditions, δ 13 C decreased regardless of inoculation.
- Taxon
- micropropagated cassavae5
- Treatment
- water-deficient conditionse4
- Outcome
- δ 13 Ce4
- Direction
- decreasede4
Not reportedComparator
Verbatim
e4“In water-deficient conditions, regardless of inoculation, δ 13 C decreased, suggesting the dominant role of water availability in carbon assimilation”
e5“the growth of two varieties of micropropagated cassava”
Claim · model-written summary
Lowered δ 15 N and N concentrations in AMF-inoculated cassava are due to N dilution from a 63% biomass increase.
Verbatim
e3“with lowered δ 15 N and N concentrations due to N dilution from a 63% biomass increase”
e5“the growth of two varieties of micropropagated cassava”
Claim · model-written summary
Proper nitrogen management is essential for micropropagated cassava with AMF, because enhanced growth from higher root colonization could lead to N deficiency.
Verbatim
e7“to fully harness the benefits of AMF for micropropagated cassava, proper nitrogen management is essential, as the enhanced growth conferred by increased root colonization rate could lead to N deficiency”
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