Paper · 2026 · Future Postharvest and Food

Control of Postharvest Brown Rot ( Monilinia laxa ) Disease Through Copper Sulfate and Antagonistic Bacterial Combinations

Kenza Bouzoubaa; Rachid Ezzouggari; Salah‐Eddine Laasli; et al.

5
claims
15
verbatim quotes
0
citations
2
research areas
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Open access · cc-by

Abstract

ABSTRACT Brown rot, caused by Monilinia laxa , severely affects stone fruit storage, prompting the search for eco‐friendly control alternatives. This study evaluated the effectiveness of Bacillus amyloliquefaciens (B10W10) and Pseudomonas sp. (B11W11), alone or combined with copper(II) sulfate, in controlling M. laxa infections postharvest. In vitro trials revealed significant fungal inhibition (17.50%–93.98%) with copper(II) sulfate and bacterial combinations. In vivo tests on nectarines showed reduced disease severity (5.74%–63.39%) after 5 days at 22°C, with 2% copper(II) sulfate plus B. amyloliquefaciens (B10W10) proving most effective. ATR‐FTIR spectroscopy confirmed biochemical changes in fungal biomass and nectarines. These findings support copper(II) sulfate, particularly in combination with B. amyloliquefaciens , as a practical alternative to conventional fungicides.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 5

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Copper(II) sulfate and bacterial combinations inhibited Monilinia laxa growth in vitro

Treatment
copper(II) sulfate and bacterial combinationse3
Interactor
Monilinia laxaMonilinia laxae0pathogen
Second interactor
Bacillus amyloliquefaciens (B10W10)Bacillus amyloliquefaciense1biocontrol_agent
Outcome
fungal inhibitione3
Direction
inhibitione3
Value
17.50%–93.98%e3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus persica var. nucipersica
Prunus persica var. nucipersica · Taxon · picture of the speciesAllagash Brewing · CC BY 2.0 · Wikimedia Commons ↗
Monilinia laxa
Monilinia laxa · InteractorArthuc01 · CC BY-SA 3.0 · Wikimedia Commons ↗
Bacillus amyloliquefaciens
Bacillus amyloliquefaciens · Second interactorAgricultural Research Service (NRRL)Culture Collection · Public domain · Wikimedia Commons ↗

Verbatim

e0

“caused by Monilinia laxa”

✓ located verbatim in the source · abstract, characters 20–44 · show in text ↑
e1

“This study evaluated the effectiveness of Bacillus amyloliquefaciens (B10W10) and Pseudomonas sp. (B11W11), alone or combined with copper(II) sulfate, in controlling M. laxa infections postharvest”

✓ located verbatim in the source · abstract, characters 145–341 · show in text ↑
e2

“In vivo tests on nectarines”

✓ located verbatim in the source · abstract, characters 466–493 · show in text ↑
e3

“In vitro trials revealed significant fungal inhibition (17.50%–93.98%) with copper(II) sulfate and bacterial combinations”

✓ located verbatim in the source · abstract, characters 343–464 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Bacillus amyloliquefaciens (B10W10) and Pseudomonas sp. (B11W11), alone or combined with copper(II) sulfate, reduced disease severity of Monilinia laxa on nectarines in vivo

Treatment
Bacillus amyloliquefaciens (B10W10) and Pseudomonas sp. (B11W11), alone or combined with copper(II) sulfatee1
Interactor
Monilinia laxaMonilinia laxae0pathogen
Second interactor
Bacillus amyloliquefaciens (B10W10)Bacillus amyloliquefaciense1biocontrol_agent
Outcome
disease severitye4
Direction
reducede4
Value
5.74%–63.39%e4
Qualifier
after 5 days at 22°Ce4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus persica var. nucipersica
Prunus persica var. nucipersica · Taxon · picture of the speciesAllagash Brewing · CC BY 2.0 · Wikimedia Commons ↗
Monilinia laxa
Monilinia laxa · InteractorArthuc01 · CC BY-SA 3.0 · Wikimedia Commons ↗
Bacillus amyloliquefaciens
Bacillus amyloliquefaciens · Second interactorAgricultural Research Service (NRRL)Culture Collection · Public domain · Wikimedia Commons ↗

Verbatim

e0

“caused by Monilinia laxa”

✓ located verbatim in the source · abstract, characters 20–44 · show in text ↑
e1

“This study evaluated the effectiveness of Bacillus amyloliquefaciens (B10W10) and Pseudomonas sp. (B11W11), alone or combined with copper(II) sulfate, in controlling M. laxa infections postharvest”

✓ located verbatim in the source · abstract, characters 145–341 · show in text ↑
e2

“In vivo tests on nectarines”

✓ located verbatim in the source · abstract, characters 466–493 · show in text ↑
e4

“showed reduced disease severity (5.74%–63.39%) after 5 days at 22°C, with 2% copper(II) sulfate plus B. amyloliquefaciens (B10W10) proving most effective”

✓ located verbatim in the source · abstract, characters 494–647 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

2% copper(II) sulfate plus B. amyloliquefaciens (B10W10) was the most effective treatment against Monilinia laxa on nectarines

Treatment
2% copper(II) sulfate plus B. amyloliquefaciens (B10W10)e4
Interactor
Monilinia laxaMonilinia laxae0pathogen
Second interactor
B. amyloliquefaciens (B10W10)Bacillus amyloliquefaciense4biocontrol_agent
Outcome
most effectivee4
Direction
most effectivee4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus persica var. nucipersica
Prunus persica var. nucipersica · Taxon · picture of the speciesAllagash Brewing · CC BY 2.0 · Wikimedia Commons ↗
Monilinia laxa
Monilinia laxa · InteractorArthuc01 · CC BY-SA 3.0 · Wikimedia Commons ↗
Bacillus amyloliquefaciens
Bacillus amyloliquefaciens · Second interactorAgricultural Research Service (NRRL)Culture Collection · Public domain · Wikimedia Commons ↗

Verbatim

e0

“caused by Monilinia laxa”

✓ located verbatim in the source · abstract, characters 20–44 · show in text ↑
e2

“In vivo tests on nectarines”

✓ located verbatim in the source · abstract, characters 466–493 · show in text ↑
e4

“showed reduced disease severity (5.74%–63.39%) after 5 days at 22°C, with 2% copper(II) sulfate plus B. amyloliquefaciens (B10W10) proving most effective”

✓ located verbatim in the source · abstract, characters 494–647 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

ATR-FTIR spectroscopy confirmed biochemical changes in fungal biomass and in nectarines

Outcome
biochemical changes in fungal biomasse5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus persica var. nucipersica
Prunus persica var. nucipersica · Taxon · picture of the speciesAllagash Brewing · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e5

“confirmed biochemical changes in fungal biomass and nectarines”

✓ located verbatim in the source · abstract, characters 671–733 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

Copper(II) sulfate, particularly in combination with B. amyloliquefaciens, is a practical alternative to conventional fungicides for postharvest control

Outcome
as a practical alternative to conventional fungicidese7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus persica var. nucipersica
Prunus persica var. nucipersica · Taxon · picture of the speciesAllagash Brewing · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e2

“In vivo tests on nectarines”

✓ located verbatim in the source · abstract, characters 466–493 · show in text ↑
e6

“These findings support copper(II) sulfate, particularly in combination with B. amyloliquefaciens”

✓ located verbatim in the source · abstract, characters 735–831 · show in text ↑
e7

“as a practical alternative to conventional fungicides”

✓ located verbatim in the source · abstract, characters 834–887 · show in text ↑