Abstract
Polygonatum odoratum, a valuable medicinal herb, faces severe cultivation constraints due to continuous cropping (CC) obstacles, yet the physiological and molecular mechanisms in its rhizome remain unclear. This study integrates physiological and transcriptomic analyses comparing CC and first-cropping plants. Under CC stress, rhizomes exhibited reduced diameter, root length, and root number, along with decreased antioxidant enzyme activities and polysaccharide content, but increased malondialdehyde levels and 3 types of phenolic acids. RNA-seq identified 3357 upregulated and 1215 downregulated DEGs. KEGG analysis revealed downregulation in plant hormone signal transduction, particularly in IAA-related genes, while the JA, CTK, and ABA pathways, along with key carbohydrate metabolism processes, were upregulated. Moreover, exogenous NAA application promotes the root growth in the rhizome of P. odoratum. These results uncover hormone signaling reprogramming and metabolic shifts underlying CC responses, offering novel insights and a theoretical basis for mitigating cropping obstacles in medicinal plants.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
Continuous cropping reduced rhizome diameter, root length and root number.
- Organ / stage
- rhizomese0
- Treatment
- CC stresse0
- Outcome
- diameter, root length, and root numbere0
- Direction
- reducede0
Not reportedTaxonComparator
Verbatim
e0“Under CC stress, rhizomes exhibited reduced diameter, root length, and root number”
Claim · model-written summary
Continuous cropping decreased antioxidant enzyme activities and polysaccharide content.
Not reportedTaxonTreatmentComparator
Verbatim
e0“decreased antioxidant enzyme activities and polysaccharide content”
Claim · model-written summary
Continuous cropping increased malondialdehyde.
Not reportedTaxonTreatmentComparator
Verbatim
e0“increased malondialdehyde levels and 3 types of phenolic acids”
Claim · model-written summary
Continuous cropping increased three phenolic acid types.
Not reportedTaxonTreatmentComparator
Verbatim
e0“increased malondialdehyde levels and 3 types of phenolic acids”
Claim · model-written summary
RNA sequencing identified 3357 upregulated DEGs.
Not reportedTaxon
Verbatim
e0“RNA-seq identified 3357 upregulated and 1215 downregulated DEGs”
Claim · model-written summary
RNA sequencing identified 1215 downregulated DEGs.
Not reportedTaxon
Verbatim
e0“RNA-seq identified 3357 upregulated and 1215 downregulated DEGs”
Claim · model-written summary
Plant hormone signal transduction and IAA related genes were downregulated.
Not reportedTaxon
Verbatim
e0“downregulation in plant hormone signal transduction, particularly in IAA-related genes”
Claim · model-written summary
JA, CTK, ABA and carbohydrate metabolism pathways were upregulated.
Not reportedTaxon
Verbatim
e0“the JA, CTK, and ABA pathways, along with key carbohydrate metabolism processes, were upregulated”
Claim · model-written summary
Exogenous NAA promoted root growth in rhizomes.
- Taxon
- P. odoratum
Polygonatum odoratume0 - Organ / stage
- rhizomee0
- Treatment
- exogenous NAA applicatione0
- Outcome
- root growthe0
- Direction
- promotese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenous NAA application promotes the root growth in the rhizome of P. odoratum”