Abstract
Introduction Sweet potato ( Ipomoea batatas (L.) Lam.) is an important food, feed, and industrial crop with high tolerance to marginal environments, yet its complex hexaploid genome limits molecular understanding of stress tolerance mechanisms. DC1 domain proteins, characterized by cysteine/histidine-rich (CHR) zinc-binding motifs, have been implicated in diverse regulatory processes in plants, but their evolutionary features and biological functions remain largely unknown in sweet potato. Methods We conducted a genome-wide analysis of the CHR gene family in cultivated sweet potato and its two diploid relatives, Ipomoea trifida and Ipomoea triloba , including phylogenetic, structural, synteny, promoter cis-element, and expression analyses (transcriptome and qRT-PCR). We further performed functional validation of a candidate gene by overexpression in sweet potato and assessed growth and physiological responses under salt stress. Results Twelve CHR genes were identified in I. batatas and I. triloba , and eleven in I. trifida . These genes showed conserved gene structures, motif compositions, and syntenic relationships, with segmental duplication contributing to family expansion. Promoter analysis revealed abundant cis-acting elements related to hormone signaling and abiotic stress. Expression analyses demonstrated tissue-specific patterns and strong responses to salt, drought, ABA, and JA treatments. IbCHR10 was rapidly and strongly induced by salt stress, particularly in a salt-tolerant cultivar. Overexpression of IbCHR10 enhanced salt tolerance, evidenced by improved growth, reduced oxidative damage, increased antioxidant enzyme activity, enhanced osmotic adjustment, and elevated ABA and JA accumulation under salt stress. Discussion This study provides a systematic characterization of DC1 domain (CHR) proteins in Ipomoea species and identifies IbCHR10 as an important regulator associated with salt stress tolerance, offering valuable genetic resources for developing stress-resilient sweet potato cultivars.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 14
Claim · model-written summary
Twelve CHR genes were identified in I. batatas.
- Taxon
- I. batatas
_Flower.jpg?width=60)
Ipomoea batatase0 - Gene
- CHRe0
- Outcome
- genese0
- Value
- Twelvee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Flower.jpg?width=500)
Verbatim
e0“Twelve CHR genes were identified in I. batatas and I. triloba , and eleven in I. trifida”
Claim · model-written summary
Twelve CHR genes were identified in I. triloba.
- Taxon
- I. triloba
Ipomoea trilobae0 - Gene
- CHRe0
- Outcome
- genese0
- Value
- Twelvee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Twelve CHR genes were identified in I. batatas and I. triloba , and eleven in I. trifida”
Claim · model-written summary
Eleven CHR genes were identified in I. trifida.
- Taxon
- I. trifida
Ipomoea trifidae0 - Gene
- CHRe0
- Outcome
- genese0
- Value
- elevene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Twelve CHR genes were identified in I. batatas and I. triloba , and eleven in I. trifida”
Claim · model-written summary
CHR genes showed conserved structure, motifs and synteny.
- Gene
- genese0
- Outcome
- gene structures, motif compositions, and syntenic relationshipse0
- Direction
- conservede0
IpomoeaOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These genes showed conserved gene structures, motif compositions, and syntenic relationships”
e1“Ipomoea species”
Claim · model-written summary
Segmental duplication contributed to CHR family expansion.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“with segmental duplication contributing to family expansion”
e1“Ipomoea species”
Claim · model-written summary
CHR promoters contained abundant hormone and abiotic stress response elements.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Promoter analysis revealed abundant cis-acting elements related to hormone signaling and abiotic stress”
e1“Ipomoea species”
Claim · model-written summary
CHR gene expression showed tissue-specific patterns.
- Outcome
- tissue-specific patternse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression analyses demonstrated tissue-specific patterns and strong responses to salt, drought, ABA, and JA treatments”
e1“Ipomoea species”
Claim · model-written summary
CHR gene expression responded strongly to salt, drought, ABA and JA treatments.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression analyses demonstrated tissue-specific patterns and strong responses to salt, drought, ABA, and JA treatments”
e1“Ipomoea species”
Claim · model-written summary
Salt stress rapidly and strongly induced IbCHR10, especially in a salt-tolerant cultivar.
- Taxon
- sweet potatoIpomoea batatase1
- Treatment
- salt stresse0
- Gene
- IbCHR10e0
- Outcome
- IbCHR10e0
- Direction
- rapidly and strongly inducede0
- Qualifier
- particularly in a salt-tolerant cultivare0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IbCHR10 was rapidly and strongly induced by salt stress, particularly in a salt-tolerant cultivar”
e1“sweet potato”
Claim · model-written summary
IbCHR10 overexpression improved sweet-potato growth under salt stress.
- Taxon
- sweet potatoIpomoea batatase1
- Treatment
- Overexpression of IbCHR10e0
- Gene
- IbCHR10e0
- Outcome
- growthe0
- Direction
- improvede0
- Qualifier
- under salt stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of IbCHR10 enhanced salt tolerance, evidenced by improved growth, reduced oxidative damage, increased antioxidant enzyme activity, enhanced osmotic adjustment, and elevated ABA and JA accumulation under salt stress”
e1“sweet potato”
Claim · model-written summary
IbCHR10 overexpression reduced oxidative damage under salt stress.
- Taxon
- sweet potatoIpomoea batatase1
- Treatment
- Overexpression of IbCHR10e0
- Gene
- IbCHR10e0
- Outcome
- oxidative damagee0
- Direction
- reducede0
- Qualifier
- under salt stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of IbCHR10 enhanced salt tolerance, evidenced by improved growth, reduced oxidative damage, increased antioxidant enzyme activity, enhanced osmotic adjustment, and elevated ABA and JA accumulation under salt stress”
e1“sweet potato”
Claim · model-written summary
IbCHR10 overexpression increased antioxidant enzyme activity under salt stress.
- Taxon
- sweet potatoIpomoea batatase1
- Treatment
- Overexpression of IbCHR10e0
- Gene
- IbCHR10e0
- Outcome
- antioxidant enzyme activitye0
- Direction
- increasede0
- Qualifier
- under salt stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of IbCHR10 enhanced salt tolerance, evidenced by improved growth, reduced oxidative damage, increased antioxidant enzyme activity, enhanced osmotic adjustment, and elevated ABA and JA accumulation under salt stress”
e1“sweet potato”
Claim · model-written summary
IbCHR10 overexpression enhanced osmotic adjustment under salt stress.
- Taxon
- sweet potatoIpomoea batatase1
- Treatment
- Overexpression of IbCHR10e0
- Gene
- IbCHR10e0
- Outcome
- osmotic adjustmente0
- Direction
- enhancede0
- Qualifier
- under salt stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of IbCHR10 enhanced salt tolerance, evidenced by improved growth, reduced oxidative damage, increased antioxidant enzyme activity, enhanced osmotic adjustment, and elevated ABA and JA accumulation under salt stress”
e1“sweet potato”
Claim · model-written summary
IbCHR10 overexpression elevated ABA and JA accumulation under salt stress.
- Taxon
- sweet potatoIpomoea batatase1
- Treatment
- Overexpression of IbCHR10e0
- Gene
- IbCHR10e0
- Outcome
- ABA and JA accumulatione0
- Direction
- elevatede0
- Qualifier
- under salt stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of IbCHR10 enhanced salt tolerance, evidenced by improved growth, reduced oxidative damage, increased antioxidant enzyme activity, enhanced osmotic adjustment, and elevated ABA and JA accumulation under salt stress”
e1“sweet potato”