Paper · 2026 · Blue Biotechnology

Seed treatment with seaweed extract biofertilizers and foliar application of blended microalgae extracts enhanced barley (Hordeum vulgare) crop yield

Vinícius Cordeiro de Moraes; Lylian Barbosa Ruivo; Rafael Garcia Lopes; et al.

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Open access · cc-by-nc-nd

Abstract

Barley (Hordeum vulgare) is a versatile cereal crop known for its high productivity and nutritional value. It plays a crucial role in ensuring food security worldwide. Sustainable solutions are needed to enhance barley yields. This underscores the need for sustainable solutions to enhance barley yields. The aim of this field study was to assess the impact of two commercial mineral fertilizers (referred to as FR and FG) containing seaweed extract (Kappaphycus alvarezii and Ascophyllum nodosum) in combination with aqueous extracts of blended microalgae (Arthrospira platensis, Tetradesmus obliquus, and Chaetoceros muelleri) on barley growth and yield. A randomized block design with four replications was employed in a 3 × 3 factorial arrangement, with three seed treatments (control, commercial FR, and aqueous extracts of blended microalgae) as the primary factor and three foliar treatments (control, commercial FG, and blended microalgae aqueous extracts) as the secondary factor, resulting in 36 experimental units. Seed treatment with commercial FR increased the number of tillers per plant by 19.37% and plant height by 8.47%; but led to a 10.80% decrease in ear length and a 5.43% decrease in 100-grain weight. Applying commercial FR via seeds increased barley yield by 32.22% when combined with foliar application of the microalgae blend, and by 32.85% when combined with commercial FG via foliar application compared to the control. The combination of commercial FR seed treatment with commercial FG or aqueous extracts of blended microalgae increased barley yield by 49.73% (784.24 kg ha–1) and 50.53% (788.84 kg ha–1), respectively. This study suggests that using macroalgae-based products with blended microalgae extract is a promising sustainable approach to enhance barley development and productivity.

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Claims 8

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

FR seed treatment increased barley tillers per plant by 19.37%.

Treatment
Seed treatment with commercial FRe0
Outcome
number of tillers per plante0
Direction
increasede0
Value
19.37%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Seed treatment with commercial FR increased the number of tillers per plant by 19.37%”

✓ located verbatim in the source · abstract, characters 1026–1111 · show in text ↑
t0

“Seed treatment with seaweed extract biofertilizers and foliar application of blended microalgae extracts enhanced barley (Hordeum vulgare) crop yield”

✓ located verbatim in the source · title, characters 0–149 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

FR seed treatment increased barley plant height by 8.47%.

Treatment
Seed treatment with commercial FRe0
Outcome
plant heighte0
Direction
increasede0
Value
8.47%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Seed treatment with commercial FR increased the number of tillers per plant by 19.37% and plant height by 8.47%”

✓ located verbatim in the source · abstract, characters 1026–1137 · show in text ↑
t0

“Seed treatment with seaweed extract biofertilizers and foliar application of blended microalgae extracts enhanced barley (Hordeum vulgare) crop yield”

✓ located verbatim in the source · title, characters 0–149 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

FR seed treatment decreased barley ear length by 10.80%.

Treatment
Seed treatment with commercial FRe0
Outcome
ear lengthe0
Direction
decreasee0
Value
10.80%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Seed treatment with commercial FR increased the number of tillers per plant by 19.37% and plant height by 8.47%; but led to a 10.80% decrease in ear length”

✓ located verbatim in the source · abstract, characters 1026–1181 · show in text ↑
t0

“Seed treatment with seaweed extract biofertilizers and foliar application of blended microalgae extracts enhanced barley (Hordeum vulgare) crop yield”

✓ located verbatim in the source · title, characters 0–149 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

FR seed treatment decreased barley 100-grain weight by 5.43%.

Treatment
Seed treatment with commercial FRe0
Outcome
100-grain weighte0
Direction
decreasee0
Value
5.43%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Seed treatment with commercial FR increased the number of tillers per plant by 19.37% and plant height by 8.47%; but led to a 10.80% decrease in ear length and a 5.43% decrease in 100-grain weight”

✓ located verbatim in the source · abstract, characters 1026–1222 · show in text ↑
t0

“Seed treatment with seaweed extract biofertilizers and foliar application of blended microalgae extracts enhanced barley (Hordeum vulgare) crop yield”

✓ located verbatim in the source · title, characters 0–149 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

FR seed treatment plus foliar microalgae blend increased barley yield by 32.22% relative to control.

Treatment
commercial FR via seedse0
Outcome
barley yielde0
Direction
increasede0
Value
32.22%e0
Qualifier
foliar application of the microalgae blende0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Applying commercial FR via seeds increased barley yield by 32.22% when combined with foliar application of the microalgae blend, and by 32.85% when combined with commercial FG via foliar application compared to the control”

✓ located verbatim in the source · abstract, characters 1224–1446 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

FR seed treatment plus foliar FG increased barley yield by 32.85% relative to control.

Treatment
commercial FR via seedse0
Outcome
barley yielde0
Direction
increasede0
Value
32.85%e0
Qualifier
commercial FG via foliar applicatione0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Applying commercial FR via seeds increased barley yield by 32.22% when combined with foliar application of the microalgae blend, and by 32.85% when combined with commercial FG via foliar application compared to the control”

✓ located verbatim in the source · abstract, characters 1224–1446 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

FR seed treatment with foliar FG increased barley yield by 49.73%, reported as 784.24 kg ha–1.

Treatment
commercial FR seed treatment with commercial FGe0
Outcome
barley yielde0
Direction
increasede0
Value
49.73% (784.24 kg ha–1)e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The combination of commercial FR seed treatment with commercial FG or aqueous extracts of blended microalgae increased barley yield by 49.73% (784.24 kg ha–1) and 50.53% (788.84 kg ha–1), respectively”

✓ located verbatim in the source · abstract, characters 1448–1648 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

FR seed treatment with foliar blended microalgae extracts increased barley yield by 50.53%, reported as 788.84 kg ha–1.

Treatment
commercial FR seed treatment with commercial FG or aqueous extracts of blended microalgaee0
Outcome
barley yielde0
Direction
increasede0
Value
50.53% (788.84 kg ha–1)e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The combination of commercial FR seed treatment with commercial FG or aqueous extracts of blended microalgae increased barley yield by 49.73% (784.24 kg ha–1) and 50.53% (788.84 kg ha–1), respectively”

✓ located verbatim in the source · abstract, characters 1448–1648 · show in text ↑

botanical · yield · Hordeum vulgare (barley)Positive and negative results

13 papers: 12 positive, 1 negative or no effect

11 papers in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
  3. positive

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seed treatment · yield · Hordeum vulgare (barley)Positive and negative results

49 papers: 43 positive, 6 negative or no effect

42 papers in the same direction as this paper · 5 in the opposite direction · 1 reporting no difference from an alternative

  1. no effect
  2. no effect
  3. no effect

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seed treatment · growth · Hordeum vulgare (barley)Positive and negative results

58 papers: 47 positive, 11 negative or no effect

46 papers in the same direction as this paper · 11 in the opposite direction

  1. no effect
  2. negative
  3. no effect

54 papers more in this group

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.