Paper · Institutional Repositories DataBase (IRDB)

Effect of pH and substrates on polyphenol oxidase activity of Japanese processing potatoes

Hironaka, Kazunori; Itaya, Toshihiko; Koaze, Hiroshi; et al.

4
claims
7
verbatim quotes
0
citations
2
research areas
✓
Open access

Abstract

The enzyme activity of polyphenol oxidase (PPO) for Japanese processing potatoes, Toyoshiro (bruise-resistant variety) and Norin No.1 (susceptible one), was measured at different pHs or substrates to investigate pH or substrate-specificity dependence.The pH-optimum of Toyoshiro PPO was pH 6.2-6.6,whereas that of Norin No.1 was 5.4.In addition, the PPO activity level of Norin No.1 was approximately 6 times higher (p 0.01) than that of Toyoshiro, when the specific activities were determined by using tyrosine as a substrate.This result implies that less tyrosine content of potatoes could reduce blackspot bruise.This information may be useful for breeders in developing new blackspot-bruise-resistant varieties.

Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 4

Observationobservedoriginal resultmeasured

Claim · model-written summary

Toyoshiro PPO had a pH optimum of 6.2-6.6.

Taxon
Japanese processing potatoest0
Material
Toyoshiroe0
Gene
PPOe0
Outcome
pH-optimume0
Value
pH 6.2-6.6e0

Verbatim

e0

“The pH-optimum of Toyoshiro PPO was pH 6.2-6.6,whereas that of Norin No.1 was 5.4.”

✓ located verbatim in the source · abstract, characters 251–333 · show in text ↑
t0

“Japanese processing potatoes”

✓ located verbatim in the source · title, characters 62–90 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Norin No.1 PPO had a pH optimum of 5.4.

Taxon
Japanese processing potatoest0
Material
Norin No.1e0
Gene
PPOe0
Outcome
pH-optimume0
Value
5.4e0

Verbatim

e0

“The pH-optimum of Toyoshiro PPO was pH 6.2-6.6,whereas that of Norin No.1 was 5.4.”

✓ located verbatim in the source · abstract, characters 251–333 · show in text ↑
t0

“Japanese processing potatoes”

✓ located verbatim in the source · title, characters 62–90 · show in text ↑
Observationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Norin No.1 PPO activity with tyrosine was approximately six times higher than Toyoshiro PPO activity.

Taxon
Japanese processing potatoest0
Material
Norin No.1e0
Gene
PPOe0
Outcome
PPO activity levele0
Direction
highere0
Value
approximately 6 timese0
Qualifier
using tyrosine as a substratee0
Comparator
Toyoshiroe0

Verbatim

e0

“the PPO activity level of Norin No.1 was approximately 6 times higher (p 0.01) than that of Toyoshiro, when the specific activities were determined by using tyrosine as a substrate”

✓ located verbatim in the source · abstract, characters 346–526 · show in text ↑
t0

“Japanese processing potatoes”

✓ located verbatim in the source · title, characters 62–90 · show in text ↑
Mechanismhypothesisoriginal resultpositivebasis n/a

Claim · model-written summary

The authors suggest that lower potato tyrosine content could reduce blackspot bruise.

Taxon
potatoesSolanum tuberosume0
Compound
tyrosinee0
Outcome
blackspot bruisee0
Qualifier
less tyrosine contente0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“less tyrosine content of potatoes could reduce blackspot bruise”

✓ located verbatim in the source · abstract, characters 552–615 · show in text ↑