Abstract
The development of pure homozygous lines is a critical objective in plant breeding, yet conventional methodologies for achieving this goal are time-consuming. Double haploid (DH) technology suggests a powerful technique for producing pure lines with homozygosity stabilization within a single generation. This study aimed to optimize haploid induction protocols and evaluated the effects of gamma-irradiated pollen (300, 400, 500 Gy, Co⁶⁰) on three cucumber ( Cucumis sativus L. ) genotypes (Dastjerdi, Beith Alpha, Super Dominus). Functional traits and pollen grain morphology, embryogenesis and fruit properties were evaluated under two pollination treatments (T1: simultaneous with irradiation; T2: one-day delay). The results showed that, dose of 300 Gy positively stimulated germination, pollen tube growth and haploid embryogenesis, while higher doses adversely affected pollen viability and wall thickness. Among the genotypes, ‘Dastjerdi’ exhibited superior tolerance to gamma radiation, likely attributable to its enhanced antioxidant capacity and efficient DNA repair mechanisms. Regarding pollination timing, simultaneous irradiation (T1) demonstrated higher efficacy compared to the delayed approach (T2). Strong positive correlations were identified between embryogenesis and pollen functional traits. No significant relationships were observed between pollen traits and fruit characteristics, indicating independent regulatory mechanisms. In conclusion, this study indicates that determining the optimal dose and correct pollination timing each genotype to improve DH production efficiency within cucumber breeding programs.
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Claims 9
Claim · model-written summary
At 300 Gy, irradiation stimulated pollen germination and tube growth.
- Taxon
- cucumber
Cucumis sativust0 - Treatment
- dose of 300 Gye0
- Outcome
- germination, pollen tube growthe0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“dose of 300 Gy positively stimulated germination, pollen tube growth and haploid embryogenesis”
t0“cucumber (Cucumis sativus L.)”
Claim · model-written summary
At 300 Gy, irradiation stimulated haploid embryogenesis.
- Taxon
- cucumberCucumis sativust0
- Treatment
- dose of 300 Gye0
- Outcome
- haploid embryogenesise0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“dose of 300 Gy positively stimulated germination, pollen tube growth and haploid embryogenesis”
t0“cucumber (Cucumis sativus L.)”
Claim · model-written summary
Higher irradiation doses adversely affected pollen viability and wall thickness.
- Taxon
- cucumberCucumis sativust0
- Treatment
- higher dosese0
- Outcome
- pollen viability and wall thicknesse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“higher doses adversely affected pollen viability and wall thickness”
t0“cucumber (Cucumis sativus L.)”
Claim · model-written summary
Dastjerdi had superior gamma radiation tolerance among the genotypes.
- Taxon
- cucumberCucumis sativust0
- Material
- Dastjerdie0
- Outcome
- tolerance to gamma radiatione0
- Direction
- superiore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Among the genotypes, ‘Dastjerdi’ exhibited superior tolerance to gamma radiation”
t0“cucumber (Cucumis sativus L.)”
Claim · model-written summary
The authors attribute Dastjerdi tolerance to antioxidant capacity and DNA repair.
- Taxon
- cucumberCucumis sativust0
- Material
- Dastjerdie0
- Outcome
- tolerance to gamma radiatione0
- Qualifier
- enhanced antioxidant capacity and efficient DNA repair mechanismse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“‘Dastjerdi’ exhibited superior tolerance to gamma radiation, likely attributable to its enhanced antioxidant capacity and efficient DNA repair mechanisms”
t0“cucumber (Cucumis sativus L.)”
Claim · model-written summary
Simultaneous irradiation had higher efficacy than delayed pollination.
- Taxon
- cucumberCucumis sativust0
- Treatment
- simultaneous irradiation (T1)e0
- Outcome
- efficacye0
- Direction
- highere0
- Comparator
- the delayed approach (T2)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“simultaneous irradiation (T1) demonstrated higher efficacy compared to the delayed approach (T2)”
t0“cucumber (Cucumis sativus L.)”
Claim · model-written summary
Embryogenesis and pollen functional traits were positively correlated.
- Taxon
- cucumberCucumis sativust0
- Organ / stage
- pollene0
- Outcome
- embryogenesise0
- Direction
- positivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Strong positive correlations were identified between embryogenesis and pollen functional traits”
t0“cucumber (Cucumis sativus L.)”
Claim · model-written summary
Pollen traits and fruit characteristics had no significant relationship.
- Taxon
- cucumberCucumis sativust0
- Organ / stage
- pollene0
- Outcome
- relationshipse0
- Qualifier
- fruit characteristicse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No significant relationships were observed between pollen traits and fruit characteristics”
t0“cucumber (Cucumis sativus L.)”
Claim · model-written summary
The authors recommend determining optimal irradiation dose and pollination timing for each genotype to improve doubled haploid production.
- Taxon
- cucumberCucumis sativuse0
- Treatment
- optimal dose and correct pollination timinge0
- Outcome
- improve DH production efficiencye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“determining the optimal dose and correct pollination timing each genotype to improve DH production efficiency within cucumber breeding programs”
The same questions in other papers
radiation · control · Cucumis sativus (cucumber)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.