Paper · 2026 · Agronomy

Effect of Red–Blue Light Ratios on Leaf Development and Steviol Glycoside Production at Different Growth Stages in Hydroponic Stevia

Cheng Tai Chou; Vivian Christabel; Mai Anh Le; et al.

9
claims
10
verbatim quotes
0
citations
2
research areas
✓
Open access · cc-by

Abstract

Stevia is a natural source of high-intensity sweeteners, collectively known as steviol glycosides (SG), which are approximately 300 times sweeter than sucrose and widely used as sugar substitutes. This study examines the impact of five different red-to-blue (R:B) light ratios on SG content and yield in hydroponic Stevia across four growth stages. Results indicate that the highest and lowest leaf dry weights were recorded in the R1B0 (R:B = 1:0) and R0B1 (R:B = 0:1) groups, at 2.88 and 1.98 g/plant, respectively, reflecting a 45.45% difference. The total SG content in dried leaves was highest in R0B1 (196.32 mg/g) and lowest in R1B0 (115.16 mg/g), with a 70.48% variation. The highest and lowest total SG yields (YSG) per square meter were observed in R0B1 (46.56 g/m2) and R50B37 (35.70 g/m2), differing by 30.42%. Stage-specific optimal YSG values were identified, with designated growth stages P1 (early vegetative growth phase), P2 (early leaf development phase), and P3 (late leaf development phase) favoring R4B1 and P4 (leaf senescence phase) favoring R0B1. These findings suggest an optimized lighting strategy for the four growth stages of hydroponic Stevia, sequentially applying R4B1, R4B1, R4B1 and R0B1 to enhance biomass accumulation and SG production at different developmental stages.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

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Claims 9

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

R1B0 had the highest leaf dry weight, 2.88 g/plant.

Taxon
SteviaSteviae1
Treatment
R1B0e0
Outcome
leaf dry weightse0
Direction
higheste0
Value
2.88e0
Comparator
R0B1e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Stevia
Stevia · Taxon · picture of the genus (one of its species)Ethel Aardvark · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the highest and lowest leaf dry weights were recorded in the R1B0 (R:B = 1:0) and R0B1 (R:B = 0:1) groups, at 2.88 and 1.98 g/plant, respectively”

✓ located verbatim in the source · abstract, characters 371–516 · show in text ↑
e1

“These findings suggest an optimized lighting strategy for the four growth stages of hydroponic Stevia, sequentially applying R4B1, R4B1, R4B1 and R0B1 to enhance biomass accumulation and SG production at different developmental stages”

✓ located verbatim in the source · abstract, characters 1072–1306 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

R0B1 had the lowest leaf dry weight, 1.98 g/plant.

Treatment
R0B1e0
Outcome
leaf dry weightse0
Direction
loweste0
Value
1.98 g/plante0
Comparator
R1B0e0

Not reportedTaxon

Verbatim

e0

“the highest and lowest leaf dry weights were recorded in the R1B0 (R:B = 1:0) and R0B1 (R:B = 0:1) groups, at 2.88 and 1.98 g/plant, respectively”

✓ located verbatim in the source · abstract, characters 371–516 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

R0B1 produced the highest total SG content, 196.32 mg/g.

Treatment
R0B1e0
Outcome
total SG content in dried leavese0
Direction
higheste0
Value
196.32 mg/ge0
Comparator
R1B0e0

Not reportedTaxon

Verbatim

e0

“The total SG content in dried leaves was highest in R0B1 (196.32 mg/g) and lowest in R1B0 (115.16 mg/g)”

✓ located verbatim in the source · abstract, characters 550–653 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

R1B0 produced the lowest total SG content, 115.16 mg/g.

Treatment
R1B0e0
Outcome
total SG content in dried leavese0
Direction
loweste0
Value
115.16 mg/ge0
Comparator
R0B1e0

Not reportedTaxon

Verbatim

e0

“The total SG content in dried leaves was highest in R0B1 (196.32 mg/g) and lowest in R1B0 (115.16 mg/g)”

✓ located verbatim in the source · abstract, characters 550–653 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

R0B1 had the highest total SG yield per square meter, 46.56 g/m2.

Treatment
R0B1e0
Outcome
total SG yields (YSG) per square metere0
Direction
higheste0
Value
46.56 g/m2e0
Comparator
R50B37e0

Not reportedTaxon

Verbatim

e0

“The highest and lowest total SG yields (YSG) per square meter were observed in R0B1 (46.56 g/m2) and R50B37 (35.70 g/m2)”

✓ located verbatim in the source · abstract, characters 680–800 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

R50B37 had the lowest total SG yield per square meter, 35.70 g/m2.

Treatment
R50B37e0
Outcome
total SG yields (YSG) per square metere0
Direction
loweste0
Value
35.70 g/m2e0
Comparator
R0B1e0

Not reportedTaxon

Verbatim

e0

“The highest and lowest total SG yields (YSG) per square meter were observed in R0B1 (46.56 g/m2) and R50B37 (35.70 g/m2)”

✓ located verbatim in the source · abstract, characters 680–800 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

R4B1 favored optimal YSG in stages P1, P2, and P3.

Organ / stage
P1 (early vegetative growth phase), P2 (early leaf development phase), and P3 (late leaf development phase)e0
Treatment
R4B1e0
Outcome
optimal YSG valuese0

Not reportedTaxon

Verbatim

e0

“Stage-specific optimal YSG values were identified, with designated growth stages P1 (early vegetative growth phase), P2 (early leaf development phase), and P3 (late leaf development phase) favoring R4B1”

✓ located verbatim in the source · abstract, characters 823–1025 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

R0B1 favored optimal YSG at the P4 leaf senescence phase.

Organ / stage
P4 (leaf senescence phase)e0
Treatment
R0B1e0
Outcome
favoring R0B1e0

Not reportedTaxon

Verbatim

e0

“P4 (leaf senescence phase) favoring R0B1”

✓ located verbatim in the source · abstract, characters 1030–1070 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The authors suggest sequential R4B1, R4B1, R4B1, and R0B1 lighting across four growth stages.

Taxon
SteviaSteviae0
Outcome
sequentially applying R4B1, R4B1, R4B1 and R0B1e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Stevia
Stevia · Taxon · picture of the genus (one of its species)Ethel Aardvark · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e0

“These findings suggest an optimized lighting strategy for the four growth stages of hydroponic Stevia, sequentially applying R4B1, R4B1, R4B1 and R0B1 to enhance biomass accumulation and SG production at different developmental stages”

✓ located verbatim in the source · abstract, characters 1072–1306 · show in text ↑