Abstract
Forage grasses are an important component of livestock systems due to their contribution to animal feed, soil conservation, and carbon sequestration. In the face of climate change, analyzing stomatal characteristics allows us to understand the mechanisms of adaptation and tolerance to environmental stress. Therefore, the objective of this study was to determine the stomatal characteristics and trichome density of ten forage grasses present in a pine-oak dominated ecosystem. Sampling was carried out in October and November 2022 on a 1938 ha area. Mature, healthy leaves were selected, and epidermal impressions were obtained from the adaxial and abaxial surfaces using the cyanoacrylate method. Observations were made with an optical microscope at 400× magnification, quantifying stomatal density, trichome density, number of epidermal cells, and stomatal index per mm2. The results indicated that nine species were amphistomatic, while Schizachyrium scoparium exhibited an epistomatic pattern. Muhlenbergia arizonica showed the highest stomatal density, and Setaria parviflora the lowest. It is concluded that there is high stomatal variability among species, highlighting its importance for the management and improvement of pastures.
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Claims 5
Claim · model-written summary
Nine forage grass species were amphistomatic.
Verbatim
e1“ten forage grasses present in a pine-oak dominated ecosystem”
e2“nine species were amphistomatic”
Claim · model-written summary
Schizachyrium scoparium showed an epistomatic stomatal pattern.
- Taxon
- Schizachyrium scoparium
Schizachyrium scopariume3 - Outcome
- epistomatic patterne3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e1“ten forage grasses present in a pine-oak dominated ecosystem”
e3“while Schizachyrium scoparium exhibited an epistomatic pattern”
Claim · model-written summary
Muhlenbergia arizonica had the highest stomatal density among the forage grasses studied.
- Taxon
- Muhlenbergia arizonicaMuhlenbergia arizonicae4
- Outcome
- stomatal densitye4
- Direction
- higheste4
Verbatim
e1“ten forage grasses present in a pine-oak dominated ecosystem”
e4“Muhlenbergia arizonica showed the highest stomatal density, and Setaria parviflora the lowest”
Claim · model-written summary
Setaria parviflora had the lowest stomatal density among the forage grasses studied.
- Taxon
- Setaria parviflora
Setaria parviflorae4 - Outcome
- stomatal densitye4
- Direction
- loweste4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e1“ten forage grasses present in a pine-oak dominated ecosystem”
e4“Muhlenbergia arizonica showed the highest stomatal density, and Setaria parviflora the lowest”
Claim · model-written summary
There is high stomatal variability among the forage grass species.
Verbatim
e1“ten forage grasses present in a pine-oak dominated ecosystem”
e5“there is high stomatal variability among species”