Paper · 2026 · Grasses

Stomatal Characterization of Grasses Present in an Oak-Pine Ecosystem

Jaime Neftalí Márquez-Godoy; Edith Ramírez-Segura; Abieser Vázquez-González; et al.

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verbatim quotes
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citations
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Open access · cc-by

Abstract

Forage grasses are an important component of livestock systems due to their contribution to animal feed, soil conservation, and carbon sequestration. In the face of climate change, analyzing stomatal characteristics allows us to understand the mechanisms of adaptation and tolerance to environmental stress. Therefore, the objective of this study was to determine the stomatal characteristics and trichome density of ten forage grasses present in a pine-oak dominated ecosystem. Sampling was carried out in October and November 2022 on a 1938 ha area. Mature, healthy leaves were selected, and epidermal impressions were obtained from the adaxial and abaxial surfaces using the cyanoacrylate method. Observations were made with an optical microscope at 400× magnification, quantifying stomatal density, trichome density, number of epidermal cells, and stomatal index per mm2. The results indicated that nine species were amphistomatic, while Schizachyrium scoparium exhibited an epistomatic pattern. Muhlenbergia arizonica showed the highest stomatal density, and Setaria parviflora the lowest. It is concluded that there is high stomatal variability among species, highlighting its importance for the management and improvement of pastures.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 5

Observationobservedoriginal resultmeasured

Claim · model-written summary

Nine forage grass species were amphistomatic.

Taxon
forage grassese1
Outcome
amphistomatice2

Verbatim

e1

“ten forage grasses present in a pine-oak dominated ecosystem”

✓ located verbatim in the source · abstract, characters 417–477 · show in text ↑
e2

“nine species were amphistomatic”

✓ located verbatim in the source · abstract, characters 903–934 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Schizachyrium scoparium showed an epistomatic stomatal pattern.

Taxon
Schizachyrium scopariumSchizachyrium scopariume3
Outcome
epistomatic patterne3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Schizachyrium scoparium
Schizachyrium scoparium · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e1

“ten forage grasses present in a pine-oak dominated ecosystem”

✓ located verbatim in the source · abstract, characters 417–477 · show in text ↑
e3

“while Schizachyrium scoparium exhibited an epistomatic pattern”

✓ located verbatim in the source · abstract, characters 936–998 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Muhlenbergia arizonica had the highest stomatal density among the forage grasses studied.

Taxon
Muhlenbergia arizonicaMuhlenbergia arizonicae4
Outcome
stomatal densitye4
Direction
higheste4

Verbatim

e1

“ten forage grasses present in a pine-oak dominated ecosystem”

✓ located verbatim in the source · abstract, characters 417–477 · show in text ↑
e4

“Muhlenbergia arizonica showed the highest stomatal density, and Setaria parviflora the lowest”

✓ located verbatim in the source · abstract, characters 1000–1093 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Setaria parviflora had the lowest stomatal density among the forage grasses studied.

Taxon
Setaria parvifloraSetaria parviflorae4
Outcome
stomatal densitye4
Direction
loweste4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Setaria parviflora
Setaria parviflora · TaxonForest & Kim Starr · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e1

“ten forage grasses present in a pine-oak dominated ecosystem”

✓ located verbatim in the source · abstract, characters 417–477 · show in text ↑
e4

“Muhlenbergia arizonica showed the highest stomatal density, and Setaria parviflora the lowest”

✓ located verbatim in the source · abstract, characters 1000–1093 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

There is high stomatal variability among the forage grass species.

Taxon
forage grassese1
Outcome
stomatal variability among speciese5
Qualifier
highe5

Verbatim

e1

“ten forage grasses present in a pine-oak dominated ecosystem”

✓ located verbatim in the source · abstract, characters 417–477 · show in text ↑
e5

“there is high stomatal variability among species”

✓ located verbatim in the source · abstract, characters 1116–1164 · show in text ↑