Abstract
Fusarium root rot, predominantly caused by Fusarium falciforme, poses a significant threat to soybean productivity globally. Microbiome-based strategies offer sustainable alternatives, but the mechanisms underlying multi-niche interactions remain elusive. Here, we found that a tolerant soybean cultivar (GXD2) coordinates spatially resolved metabolite signals to recruit beneficial microbes across the rhizosphere, root endosphere, and leaf endosphere. Specifically, formononetin and maltol selectively enrich Bacillus and Massilia in the rhizosphere; arctigenin and isovanillic acid recruit Bacillus and Streptomyces to the root endosphere; and flavonoids such as diosmetin attract Penicillium and Aspergillus to the leaf endosphere. Leveraging these interactions, we constructed different types of synthetic communities (SynComs) via top-down (host-selected taxa) and bottom-up (antagonist-based) strategies. Both SynComs suppressed root rot in susceptible cultivars, with foliar application of top-down SynComs significantly enhancing shoot growth. Transcriptomics revealed distinct modes of action: that top-down SynComs activated mitogen-activated protein kinase (MAPK)-linked terpenoid and flavonoid pathways, whereas bottom-up SynComs primarily modulated host carbon-nitrogen allocation, effectively limiting pathogen resources. Our findings unveil a "metabolite-mediated, multi-niche collaborative defense" model, presenting a robust framework for microbiome-based disease management and paving the way toward sustainable crop protection strategies.
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Claims 7
Claim · model-written summary
Formononetin and maltol enrich Bacillus and Massilia in the soybean rhizosphere.
- Taxon
- soybean
Glycine maxe0 - Material
- GXD2e0
- Interactor
- Bacilluse1symbiont
- Compound
- formononetin and maltole1
- Outcome
- Bacillus and Massiliae1
- Direction
- enriche1
- Setting
- rhizospheree1
MassiliaOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a tolerant soybean cultivar (GXD2) coordinates spatially resolved metabolite signals to recruit beneficial microbes across the rhizosphere, root endosphere, and leaf endosphere”
e1“formononetin and maltol selectively enrich Bacillus and Massilia in the rhizosphere”
Claim · model-written summary
Arctigenin and isovanillic acid recruit Bacillus and Streptomyces to the soybean root endosphere.
- Taxon
- soybeanGlycine maxe0
- Interactor
- Bacilluse2symbiont
- Second interactor
- Streptomyces
Streptomycese2symbiont - Compound
- arctigenin and isovanillic acide2
- Outcome
- Bacillus and Streptomycese2
- Direction
- recruite2
- Setting
- root endospheree2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a tolerant soybean cultivar (GXD2) coordinates spatially resolved metabolite signals to recruit beneficial microbes across the rhizosphere, root endosphere, and leaf endosphere”
e2“arctigenin and isovanillic acid recruit Bacillus and Streptomyces to the root endosphere”
Claim · model-written summary
Flavonoids such as diosmetin attract Penicillium and Aspergillus to the soybean leaf endosphere.
- Taxon
- soybeanGlycine maxe0
- Interactor
- Penicillium
Penicilliume3symbiont - Second interactor
- Aspergillus
Aspergilluse3symbiont - Compound
- flavonoids such as diosmetine3
- Outcome
- Penicillium and Aspergilluse3
- Direction
- attracte3
- Setting
- leaf endospheree3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a tolerant soybean cultivar (GXD2) coordinates spatially resolved metabolite signals to recruit beneficial microbes across the rhizosphere, root endosphere, and leaf endosphere”
e3“flavonoids such as diosmetin attract Penicillium and Aspergillus to the leaf endosphere”
Claim · model-written summary
Both synthetic communities suppressed Fusarium root rot in susceptible soybean cultivars.
- Taxon
- soybeanGlycine maxe4
- Material
- susceptible cultivarse5
- Treatment
- SynComse6
- Interactor
- Fusarium falciformeFusarium falciformee4pathogen
- Outcome
- root rote5
- Direction
- suppressede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Fusarium root rot, predominantly caused by Fusarium falciforme, poses a significant threat to soybean productivity globally”
e5“Both SynComs suppressed root rot in susceptible cultivars, with foliar application of top-down SynComs significantly enhancing shoot growth”
e6“Leveraging these interactions, we constructed different types of synthetic communities (SynComs) via top-down (host-selected taxa) and bottom-up (antagonist-based) strategies”
Claim · model-written summary
Foliar application of top-down SynComs significantly enhanced soybean shoot growth.
- Taxon
- soybeanGlycine maxe4
- Treatment
- foliar application of top-down SynComse5
- Outcome
- shoot growthe5
- Direction
- enhancinge5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Fusarium root rot, predominantly caused by Fusarium falciforme, poses a significant threat to soybean productivity globally”
e5“Both SynComs suppressed root rot in susceptible cultivars, with foliar application of top-down SynComs significantly enhancing shoot growth”
Claim · model-written summary
Top-down SynComs activated MAPK-linked terpenoid and flavonoid pathways in soybean.
- Taxon
- soybeanGlycine maxe4
- Treatment
- top-down SynComse7
- Outcome
- terpenoid and flavonoid pathwayse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Fusarium root rot, predominantly caused by Fusarium falciforme, poses a significant threat to soybean productivity globally”
e7“Transcriptomics revealed distinct modes of action: that top-down SynComs activated mitogen-activated protein kinase (MAPK)-linked terpenoid and flavonoid pathways”
Claim · model-written summary
Bottom-up SynComs primarily modulated host carbon-nitrogen allocation, limiting pathogen resources.
- Taxon
- soybeanGlycine maxe4
- Treatment
- bottom-up SynComse7
- Outcome
- host carbon-nitrogen allocatione7
- Qualifier
- limiting pathogen resourcese7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Fusarium root rot, predominantly caused by Fusarium falciforme, poses a significant threat to soybean productivity globally”
e7“whereas bottom-up SynComs primarily modulated host carbon-nitrogen allocation, effectively limiting pathogen resources”
The same questions in other papers
application · growth · Glycine max (soybean)Positive and negative results
170 papers in the same direction as this paper · 35 in the opposite direction · 1 reporting no difference from an alternative
- no effect
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