Paper · 2026 · Russian Journal of Plant Physiology

Optimizing Density and Nitrogen Management Enhances Photosynthetic Performance and Compensates for Yield Loss under Water-Limited Maize Production

L. Heying; R. Qianqian; D. Xiaomin; et al.

16
claims
34
verbatim quotes
0
citations
2
research areas
—
Not open access

Abstract

Abstract Reduced nitrogen (N) inputs inevitably limit maize yield and its components, while increased planting density can potentially boost productivity per unit area. This study investigated the compensatory interplay between reduced N application and elevated planting density on grain yield and photosynthetic performance under water-limited conditions. Reducing N decreased grain yield, kernel number per spike, and 1000-kernel weight. Higher planting densities similarly reduced kernel number and weight, yet increased grain yield through a greater spike number per area. Under a 20% irrigation reduction with traditional N, a medium-density treatment (N180D2) increased grain yield by 19.1–41.6% and spike number by 28.0–28.2% compared to a treatment combining the same water reduction with 25% less N at traditional density (N90D1). This compensation was driven by a 23.9–25.7% increase in spike number, which offset a 9.1–11.7% reduction in kernel number per spike. Physiologically, moderate density elevation under reduced irrigation and traditional N maintained superior late-season photosynthetic capacity, with increases of 6.2–7.7% in leaf greenness, 64.4–83.1% in leaf area index, and 19.8–80.7% in crop growth rate relative to low-density, resource-reduced treatments. We conclude that a strategic increase in planting density effectively compensates for yield losses from concurrent water and N reductions by sustaining photosynthetic source strength and dry matter accumulation during critical late-growth stages. These findings provide a practical agronomic framework for maintaining maize productivity in resource-constrained environments.

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The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 16

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Reducing nitrogen decreased maize grain yield.

Taxon
MaizeZea mayst0
Treatment
Reducing Ne0
Outcome
grain yielde0
Direction
decreasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Reducing N decreased grain yield, kernel number per spike, and 1000-kernel weight”

✓ located verbatim in the source · abstract, characters 358–439 · show in text ↑
t0

“Maize”

✓ located verbatim in the source · title, characters 130–135 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Reducing nitrogen decreased kernel number per spike.

Taxon
MaizeZea mayst0
Treatment
Reducing Ne0
Outcome
kernel number per spikee0
Direction
decreasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Reducing N decreased grain yield, kernel number per spike, and 1000-kernel weight”

✓ located verbatim in the source · abstract, characters 358–439 · show in text ↑
t0

“Maize”

✓ located verbatim in the source · title, characters 130–135 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Reducing nitrogen decreased 1000-kernel weight.

Taxon
MaizeZea mayst0
Treatment
Reducing Ne0
Outcome
1000-kernel weighte0
Direction
decreasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Reducing N decreased grain yield, kernel number per spike, and 1000-kernel weight”

✓ located verbatim in the source · abstract, characters 358–439 · show in text ↑
t0

“Maize”

✓ located verbatim in the source · title, characters 130–135 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Higher planting density reduced kernel number.

Taxon
MaizeZea mayst0
Treatment
Higher planting densitiese0
Outcome
kernel numbere0
Direction
reducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Higher planting densities similarly reduced kernel number and weight, yet increased grain yield through a greater spike number per area”

✓ located verbatim in the source · abstract, characters 441–576 · show in text ↑
t0

“Maize”

✓ located verbatim in the source · title, characters 130–135 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Higher planting density reduced kernel weight.

Taxon
MaizeZea mayst0
Treatment
Higher planting densitiese0
Outcome
weighte0
Direction
reducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Higher planting densities similarly reduced kernel number and weight, yet increased grain yield through a greater spike number per area”

✓ located verbatim in the source · abstract, characters 441–576 · show in text ↑
t0

“Maize”

✓ located verbatim in the source · title, characters 130–135 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Higher planting density increased grain yield.

Taxon
MaizeZea mayst0
Treatment
Higher planting densitiese0
Outcome
grain yielde0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Higher planting densities similarly reduced kernel number and weight, yet increased grain yield through a greater spike number per area”

✓ located verbatim in the source · abstract, characters 441–576 · show in text ↑
t0

“Maize”

✓ located verbatim in the source · title, characters 130–135 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Higher planting density increased spike number per area.

Taxon
MaizeZea mayst0
Treatment
Higher planting densitiese1
Outcome
spike number per areae0
Direction
greatere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“increased grain yield through a greater spike number per area”

✓ located verbatim in the source · abstract, characters 515–576 · show in text ↑
t0

“Maize”

✓ located verbatim in the source · title, characters 130–135 · show in text ↑
e1

“Higher planting densities”

✓ located verbatim in the source · abstract, characters 441–466 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

N180D2 increased grain yield by 19.1–41.6% relative to N90D1 under reduced irrigation.

Taxon
MaizeZea mayst0
Treatment
medium-density treatment (N180D2)e0
Outcome
grain yielde0
Direction
increasede0
Value
19.1–41.6%e0
Comparator
N90D1e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Under a 20% irrigation reduction with traditional N, a medium-density treatment (N180D2) increased grain yield by 19.1–41.6% and spike number by 28.0–28.2% compared to a treatment combining the same water reduction with 25% less N at traditional density (N90D1)”

✓ located verbatim in the source · abstract, characters 578–839 · show in text ↑
t0

“Maize”

✓ located verbatim in the source · title, characters 130–135 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

N180D2 increased spike number by 28.0–28.2% relative to N90D1.

Taxon
MaizeZea mayst0
Treatment
medium-density treatment (N180D2)e0
Outcome
spike numbere0
Direction
increasede0
Value
28.0–28.2%e0
Comparator
N90D1e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Under a 20% irrigation reduction with traditional N, a medium-density treatment (N180D2) increased grain yield by 19.1–41.6% and spike number by 28.0–28.2% compared to a treatment combining the same water reduction with 25% less N at traditional density (N90D1)”

✓ located verbatim in the source · abstract, characters 578–839 · show in text ↑
t0

“Maize”

✓ located verbatim in the source · title, characters 130–135 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The compensating treatment increased spike number by 23.9–25.7%.

Taxon
MaizeZea mayst0
Treatment
compensatione0
Outcome
spike numbere0
Direction
increasee0
Value
23.9–25.7%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“This compensation was driven by a 23.9–25.7% increase in spike number”

✓ located verbatim in the source · abstract, characters 841–910 · show in text ↑
t0

“Maize”

✓ located verbatim in the source · title, characters 130–135 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

The compensating treatment reduced kernel number per spike by 9.1–11.7%.

Taxon
MaizeZea mayst0
Treatment
compensatione1
Outcome
kernel number per spikee0
Direction
reductione0
Value
9.1–11.7%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“which offset a 9.1–11.7% reduction in kernel number per spike”

✓ located verbatim in the source · abstract, characters 912–973 · show in text ↑
t0

“Maize”

✓ located verbatim in the source · title, characters 130–135 · show in text ↑
e1

“This compensation was driven by a 23.9–25.7% increase in spike number”

✓ located verbatim in the source · abstract, characters 841–910 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Moderate density elevation increased leaf greenness by 6.2–7.7% relative to low-density resource-reduced treatments.

Taxon
MaizeZea mayst0
Treatment
moderate density elevation under reduced irrigation and traditional Ne0
Outcome
leaf greennesse0
Direction
increasese0
Value
6.2–7.7%e0
Comparator
low-density, resource-reduced treatmentse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“moderate density elevation under reduced irrigation and traditional N maintained superior late-season photosynthetic capacity, with increases of 6.2–7.7% in leaf greenness, 64.4–83.1% in leaf area index, and 19.8–80.7% in crop growth rate relative to low-density, resource-reduced treatments”

✓ located verbatim in the source · abstract, characters 992–1283 · show in text ↑
t0

“Maize”

✓ located verbatim in the source · title, characters 130–135 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Moderate density elevation increased leaf area index by 64.4–83.1%.

Taxon
MaizeZea mayst0
Treatment
moderate density elevation under reduced irrigation and traditional Ne0
Outcome
leaf area indexe0
Direction
increasese0
Value
64.4–83.1%e0
Comparator
low-density, resource-reduced treatmentse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“moderate density elevation under reduced irrigation and traditional N maintained superior late-season photosynthetic capacity, with increases of 6.2–7.7% in leaf greenness, 64.4–83.1% in leaf area index, and 19.8–80.7% in crop growth rate relative to low-density, resource-reduced treatments”

✓ located verbatim in the source · abstract, characters 992–1283 · show in text ↑
t0

“Maize”

✓ located verbatim in the source · title, characters 130–135 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Moderate density elevation increased crop growth rate by 19.8–80.7%.

Taxon
MaizeZea mayst0
Treatment
moderate density elevation under reduced irrigation and traditional Ne0
Outcome
crop growth ratee0
Direction
increasese0
Value
19.8–80.7%e0
Comparator
low-density, resource-reduced treatmentse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“moderate density elevation under reduced irrigation and traditional N maintained superior late-season photosynthetic capacity, with increases of 6.2–7.7% in leaf greenness, 64.4–83.1% in leaf area index, and 19.8–80.7% in crop growth rate relative to low-density, resource-reduced treatments”

✓ located verbatim in the source · abstract, characters 992–1283 · show in text ↑
t0

“Maize”

✓ located verbatim in the source · title, characters 130–135 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Moderate density elevation maintained superior late-season photosynthetic capacity.

Taxon
MaizeZea mayst0
Treatment
moderate density elevation under reduced irrigation and traditional Ne0
Outcome
late-season photosynthetic capacitye0
Direction
superiore0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“moderate density elevation under reduced irrigation and traditional N maintained superior late-season photosynthetic capacity”

✓ located verbatim in the source · abstract, characters 992–1117 · show in text ↑
t0

“Maize”

✓ located verbatim in the source · title, characters 130–135 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

The authors recommend strategically increasing planting density to compensate for yield losses under water and nitrogen reductions.

Taxon
MaizeZea mayst0
Outcome
strategic increase in planting densitye0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“a strategic increase in planting density effectively compensates for yield losses from concurrent water and N reductions by sustaining photosynthetic source strength and dry matter accumulation during critical late-growth stages”

✓ located verbatim in the source · abstract, characters 1302–1530 · show in text ↑
t0

“Maize”

✓ located verbatim in the source · title, characters 130–135 · show in text ↑

altitude · growth · Zea mays (maize)Positive and negative results

4 papers: 2 positive, 2 negative or no effect

1 paper in the same direction as this paper · 2 in the opposite direction

  1. no effect
  2. no effect
  3. positive
See all 4 papers →

irrigation · physiology: pigments · Zea mays (maize)Positive and negative results

43 papers: 28 positive, 15 negative or no effect

27 papers in the same direction as this paper · 15 in the opposite direction

  1. negative
  2. negative
  3. negative

39 papers more in this group

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irrigation · physiology: photosynthesis · Zea mays (maize)Positive and negative results

47 papers: 30 positive, 17 negative or no effect

29 papers in the same direction as this paper · 17 in the opposite direction

  1. negative
  2. negative
  3. negative

43 papers more in this group

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in vitro · yield · Zea mays (maize)Positive and negative results

52 papers: 44 positive, 8 negative or no effect

43 papers in the same direction as this paper · 7 in the opposite direction · 1 reporting no difference from an alternative

  1. negative
  2. negative
  3. negative

48 papers more in this group

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density · growth · Zea mays (maize)Positive and negative results

197 papers: 119 positive, 78 negative or no effect

77 papers in the same direction as this paper · 119 in the opposite direction

  1. positive
  2. positive
  3. positive

193 papers more in this group

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irrigation · growth · Zea mays (maize)Positive and negative results

289 papers: 208 positive, 81 negative or no effect

207 papers in the same direction as this paper · 80 in the opposite direction · 1 reporting no difference from an alternative

  1. negative
  2. no effect
  3. negative

285 papers more in this group

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density · yield · Zea mays (maize)Positive and negative results

658 papers: 519 positive, 139 negative or no effect

518 papers in the same direction as this paper · 132 in the opposite direction · 7 reporting no difference from an alternative

  1. negative
  2. negative
  3. no effect

654 papers more in this group

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.