Abstract
Abstract The present study describes an improved, efficient method for in vitro propagation of the underutilized fruit tree, Dillenia indica L. Nodal segments obtained from a mature tree were used as initial explants for in vitro regeneration. Among the various cytokinin types (BAP, Kin, m T, and TDZ) and concentrations tested, MS medium containing 0.5 mg·L −1 6-benzylaminopurine was optimal for shoot induction. Subculturing mother explants into fresh medium during subsequent passages significantly influenced shoot multiplication. A maximum of 5–7 shoots were regenerated after the third subculturing on MS medium containing 0.5 mg·L −1 6-benzylaminopurine. Maximum rooting was achieved in MS medium containing 2 mg·L −1 indole-3-butyric acid. Approximately 80% of the plants survived acclimatization. A comparative study revealed significant changes in leaf micromorphology and architecture, including developmental and structural changes in venation patterns, trichomes, and stomata, in both in vitro -grown and fully acclimatized plants. This foliar micromorphological study elucidated the mechanism of adaptation of in vitro -regenerated plantlets during acclimatization. The current investigation could provide a foundation for the large-scale propagation and conservation of this medicinally important and underutilized fruit tree species.
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Claims 8
Claim · model-written summary
MS medium containing 0.5 mg·L −1 6-benzylaminopurine was optimal for shoot induction.
- Taxon
- Dillenia indica L.
Dillenia indicat0 - Treatment
- MS medium containing 0.5 mg·L −1 6-benzylaminopurinee0
- Outcome
- shoot inductione0
- Direction
- optimale0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS medium containing 0.5 mg·L −1 6-benzylaminopurine was optimal for shoot induction”
t0“Dillenia indica L.”
Claim · model-written summary
Subculturing mother explants into fresh medium significantly influenced shoot multiplication.
- Taxon
- Dillenia indica L.Dillenia indicat0
- Treatment
- Subculturing mother explants into fresh mediume0
- Outcome
- shoot multiplicatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Subculturing mother explants into fresh medium during subsequent passages significantly influenced shoot multiplication.”
t0“Dillenia indica L.”
Claim · model-written summary
The third subculture on MS medium with 0.5 mg·L −1 6-benzylaminopurine regenerated a maximum of 5–7 shoots.
- Taxon
- Dillenia indica L.Dillenia indicat0
- Treatment
- third subculturing on MS medium containing 0.5 mg·L −1 6-benzylaminopurinee0
- Outcome
- shoots were regeneratede0
- Direction
- maximume0
- Value
- 5–7 shootse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A maximum of 5–7 shoots were regenerated after the third subculturing on MS medium containing 0.5 mg·L −1 6-benzylaminopurine.”
t0“Dillenia indica L.”
Claim · model-written summary
MS medium containing 2 mg·L −1 indole-3-butyric acid achieved maximum rooting.
- Taxon
- Dillenia indica L.Dillenia indicat0
- Treatment
- MS medium containing 2 mg·L −1 indole-3-butyric acide0
- Outcome
- rootinge0
- Direction
- Maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maximum rooting was achieved in MS medium containing 2 mg·L −1 indole-3-butyric acid.”
t0“Dillenia indica L.”
Claim · model-written summary
Approximately 80% of plants survived acclimatization.
- Taxon
- Dillenia indica L.Dillenia indicat0
- Outcome
- survived acclimatizatione0
- Value
- Approximately 80%e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Approximately 80% of the plants survived acclimatization.”
t0“Dillenia indica L.”
Claim · model-written summary
Leaf venation patterns changed significantly in vitro-grown and fully acclimatized plants.
- Taxon
- Dillenia indica L.Dillenia indicat0
- Organ / stage
- leafe0
- Outcome
- changes in venation patternse0
- Direction
- changese0
- Qualifier
- in both in vitro -grown and fully acclimatized plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“significant changes in leaf micromorphology and architecture, including developmental and structural changes in venation patterns, trichomes, and stomata, in both in vitro -grown and fully acclimatized plants”
t0“Dillenia indica L.”
Claim · model-written summary
Leaf trichomes changed significantly in vitro-grown and fully acclimatized plants.
- Taxon
- Dillenia indica L.Dillenia indicat0
- Organ / stage
- leafe0
- Outcome
- trichomese0
- Direction
- changese0
- Qualifier
- in both in vitro -grown and fully acclimatized plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“significant changes in leaf micromorphology and architecture, including developmental and structural changes in venation patterns, trichomes, and stomata, in both in vitro -grown and fully acclimatized plants”
t0“Dillenia indica L.”
Claim · model-written summary
Leaf stomata changed significantly in vitro-grown and fully acclimatized plants.
- Taxon
- Dillenia indica L.Dillenia indicat0
- Organ / stage
- leafe0
- Outcome
- stomatae0
- Direction
- changese0
- Qualifier
- in both in vitro -grown and fully acclimatized plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“significant changes in leaf micromorphology and architecture, including developmental and structural changes in venation patterns, trichomes, and stomata, in both in vitro -grown and fully acclimatized plants”
t0“Dillenia indica L.”