Paper · 2026 · JSFA reports

Biofertilization of canola seeds with Peribacillus simplex and Paenibacillus polymyxa bacteria increases tocopherol content

Pilar Martínez‐Hidalgo; José David Flores‐Félix; José M. Igual; et al.

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citations
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Open access · cc-by

Abstract

Abstract Background Biofertilization with plant growth‐promoting bacteria (PGPB) is a reliable strategy to reduce the amounts of chemical fertilizers applied to crops. Nevertheless, the effects of biofertilizers on plant chemical constituents that influence food quality and human health should be evaluated. Results Here, we analyze the effect of biofertilization with the strains of plant growth promoters Peribacillus simplex MRBN26 and Paenibacillus polymyxa MRBN45 on oil, fatty acid, and tocopherol content of canola seeds. Conclusion No statistically significant changes were found in either seed oil content or fatty acid profile after the inoculation with these strains; however, we found significant increases in the content of gamma tocopherol after the inoculation of both strains MRBN26 and MRBN45 and in alpha and total tocopherol content after the inoculation of the strain MRBN26. This is the first report about the increase of tocopherol content in canola oil through the inoculation with spore‐forming bacteria of the family Bacillaceae . The biofertilization with selected strains of these bacteria represents a good agricultural practice for enhancing the tocopherol content in canola oil which can be extended to other oil‐producing crops.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 7

Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Inoculation with both bacterial strains did not significantly change seed oil content.

Treatment
inoculation with these strainse0
Outcome
seed oil contente0
Direction
No statistically significant changese0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus var. napus
Brassica napus var. napus · Taxoncontri from Yonezawa-Shi, Yamagata, Japan · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“No statistically significant changes were found in either seed oil content or fatty acid profile after the inoculation with these strains”

✓ located verbatim in the source · abstract, characters 541–678 · show in text ↑
t0

“canola”

✓ located verbatim in the source · title, characters 20–26 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Inoculation with both bacterial strains did not significantly change fatty acid profile.

Treatment
inoculation with these strainse0
Outcome
fatty acid profilee0
Direction
No statistically significant changese0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus var. napus
Brassica napus var. napus · Taxoncontri from Yonezawa-Shi, Yamagata, Japan · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“No statistically significant changes were found in either seed oil content or fatty acid profile after the inoculation with these strains”

✓ located verbatim in the source · abstract, characters 541–678 · show in text ↑
t0

“canola”

✓ located verbatim in the source · title, characters 20–26 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Inoculation with MRBN26 increased gamma tocopherol content.

Treatment
MRBN26e0
Interactor
MRBN26e0symbiont
Outcome
gamma tocopherole0
Direction
increasese0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus var. napus
Brassica napus var. napus · Taxoncontri from Yonezawa-Shi, Yamagata, Japan · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“significant increases in the content of gamma tocopherol after the inoculation of both strains MRBN26 and MRBN45 and in alpha and total tocopherol content after the inoculation of the strain MRBN26”

✓ located verbatim in the source · abstract, characters 698–895 · show in text ↑
t0

“canola”

✓ located verbatim in the source · title, characters 20–26 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Inoculation with MRBN45 increased gamma tocopherol content.

Treatment
MRBN45e0
Interactor
MRBN45e0symbiont
Outcome
gamma tocopherole0
Direction
increasese0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus var. napus
Brassica napus var. napus · Taxoncontri from Yonezawa-Shi, Yamagata, Japan · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“significant increases in the content of gamma tocopherol after the inoculation of both strains MRBN26 and MRBN45 and in alpha and total tocopherol content after the inoculation of the strain MRBN26”

✓ located verbatim in the source · abstract, characters 698–895 · show in text ↑
t0

“canola”

✓ located verbatim in the source · title, characters 20–26 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Inoculation with MRBN26 increased alpha content.

Treatment
MRBN26e0
Interactor
MRBN26e0symbiont
Outcome
alphae0
Direction
increasese0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus var. napus
Brassica napus var. napus · Taxoncontri from Yonezawa-Shi, Yamagata, Japan · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“significant increases in the content of gamma tocopherol after the inoculation of both strains MRBN26 and MRBN45 and in alpha and total tocopherol content after the inoculation of the strain MRBN26”

✓ located verbatim in the source · abstract, characters 698–895 · show in text ↑
t0

“canola”

✓ located verbatim in the source · title, characters 20–26 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Inoculation with MRBN26 increased total tocopherol content.

Treatment
MRBN26e0
Interactor
MRBN26e0symbiont
Outcome
total tocopherole0
Direction
increasese0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus var. napus
Brassica napus var. napus · Taxoncontri from Yonezawa-Shi, Yamagata, Japan · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“significant increases in the content of gamma tocopherol after the inoculation of both strains MRBN26 and MRBN45 and in alpha and total tocopherol content after the inoculation of the strain MRBN26”

✓ located verbatim in the source · abstract, characters 698–895 · show in text ↑
t0

“canola”

✓ located verbatim in the source · title, characters 20–26 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The authors recommend selected bacterial strains to enhance canola oil tocopherol.

Treatment
biofertilization with selected strains of these bacteriae0
Outcome
enhancing the tocopherol content in canola oile0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus var. napus
Brassica napus var. napus · Taxoncontri from Yonezawa-Shi, Yamagata, Japan · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“The biofertilization with selected strains of these bacteria represents a good agricultural practice for enhancing the tocopherol content in canola oil”

✓ located verbatim in the source · abstract, characters 1057–1208 · show in text ↑

inoculation · quality: oil · Brassica napus (oilseed rape)Positive and negative results

14 papers: 13 positive, 1 negative or no effect

13 in the opposite direction

  1. positive
  2. positive
  3. positive

10 papers more in this group

See all 14 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.