Abstract
Wheat stripe (or yellow) rust, caused by Puccinia striiformis f. sp. tritici, imposes a major constraint on wheat production. While race-specific seedling resistance genes can be effective in controlling this disease, those conferring resistance at the adult stage typically protect the host from infection by a broad spectrum of the pathogen, and tend to be more durable. But the durable resistance such as that expressed by the recently developed Chinese cultivar (cv.) Jimai 262 is poorly understood. Based on the identification of a spontaneous loss-of-function mutant, a genetic analysis indicated that cv. Jimai 262's adult plant resistance to stripe rust is conferred by the presence of a single dominant gene, tentatively designated YrJ262. Bulk segregant exome sequencing implied that the distal end of chromosome arm 3BS of the mutant lacked a ~ 73 Mbp segment that was present in cv. Jimai 262, thereby placing YrJ262 within this segment. The presence of this deletion was confirmed by fluorescence in situ hybridization-based analysis. The flanking sequences of the Yr30 candidate region were identical between plants carrying Yr30 and cv. Jimai 262. YrJ262 was complemented by alleles present within QTL on cv. Jimai 262 chromosomes 1B and 4B enabling the expression of adult plant resistance.
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Claims 5
Claim · model-written summary
Adult plant resistance to stripe rust in cv. Jimai 262 is conferred by a single dominant gene, YrJ262.
- Taxon
- wheat
Triticum aestivumt0 - Material
- cv. Jimai 262e1
- Interactor
- Puccinia striiformis f. sp. tritici
Puccinia striiformis f. sp. triticie0pathogen - Gene
- YrJ262e1
- Outcome
- adult plant resistance to stripe ruste1
- Qualifier
- a single dominant genee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“a genetic analysis indicated that cv. Jimai 262's adult plant resistance to stripe rust is conferred by the presence of a single dominant gene, tentatively designated YrJ262”
e0“Wheat stripe (or yellow) rust, caused by Puccinia striiformis f. sp. tritici, imposes a major constraint on wheat production”
t0“A terminal deletion in wheat chromosome arm 3BS”
Claim · model-written summary
In the mutant, the distal end of chromosome arm 3BS lacks a segment of about 73 Mbp present in cv. Jimai 262.
- Taxon
- wheatTriticum aestivumt0
- Material
- spontaneous loss-of-function mutante1
- Outcome
- distal end of chromosome arm 3BSe2
- Value
- ~ 73 Mbpe2
- Comparator
- cv. Jimai 262e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Based on the identification of a spontaneous loss-of-function mutant”
e2“Bulk segregant exome sequencing implied that the distal end of chromosome arm 3BS of the mutant lacked a ~ 73 Mbp segment that was present in cv. Jimai 262, thereby placing YrJ262 within this segment”
t0“A terminal deletion in wheat chromosome arm 3BS”
Claim · model-written summary
The 3BS deletion was confirmed by fluorescence in situ hybridization.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- presence of this deletione3
- Qualifier
- confirmed by fluorescence in situ hybridization-based analysise3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“The presence of this deletion was confirmed by fluorescence in situ hybridization-based analysis”
t0“A terminal deletion in wheat chromosome arm 3BS”
Claim · model-written summary
The flanking sequences of the Yr30 candidate region are identical between Yr30 carriers and cv. Jimai 262.
- Taxon
- wheatTriticum aestivumt0
- Material
- cv. Jimai 262e4
- Gene
- Yr30e4
- Outcome
- flanking sequences of the Yr30 candidate regione4
- Comparator
- plants carrying Yr30e4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“The flanking sequences of the Yr30 candidate region were identical between plants carrying Yr30 and cv. Jimai 262”
t0“A terminal deletion in wheat chromosome arm 3BS”
Claim · model-written summary
YrJ262 complemented by alleles within QTL on cv. Jimai 262 chromosomes 1B and 4B enables adult plant resistance.
- Taxon
- wheatTriticum aestivumt0
- Gene
- YrJ262e5
- Outcome
- expression of adult plant resistancee5
- Qualifier
- alleles present within QTL on cv. Jimai 262 chromosomes 1B and 4Be5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“YrJ262 was complemented by alleles present within QTL on cv. Jimai 262 chromosomes 1B and 4B enabling the expression of adult plant resistance”
t0“A terminal deletion in wheat chromosome arm 3BS”