Abstract
Embryogenic cell suspension cultures were established from calli of immature seeds of Emmenopterys henryi. Callus could be obtained from immature seeds on MS solid medium augmented with 2.0 mg·L-16-benzylaminopurine (6-BA) and 3% sucrose. Embryogenic calli were induced on MS basal medium supplemented with 0.1 mg·L-1 N-acetyl aspartate (NAA) and 0.5 mg ·L-16-BA. Numerous somatic embryoids appeared on MS liquid basal nutrient medium with 0.5 mg·L-1 NAA and 0.5 mg·L-16-BA. The optimal value of the initial cell density was 2.0% (fresh weight). Several parameters that might be associated with growth were determined over a 14-day period. The embryo-derived plantlets with well-developed roots and shoots were transferred successfully to the greenhouse, with a high survival rate of 95%.
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Claims 7
Claim · model-written summary
Embryogenic cell suspension cultures were established from immature-seed calli.
- Taxon
- Emmenopterys henryi
.jpg?width=60)
Emmenopterys henryie0 - Organ / stage
- immature seedse0
- Outcome
- Embryogenic cell suspension cultures were establishede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Embryogenic cell suspension cultures were established from calli of immature seeds of Emmenopterys henryi.”
t0“Emmenopterys henryi”
Claim · model-written summary
Callus was obtained on MS solid medium with 6-BA and sucrose.
- Taxon
- Emmenopterys henryiEmmenopterys henryit0
- Organ / stage
- immature seedse0
- Treatment
- MS solid medium augmented with 2.0 mg·L-16-benzylaminopurine (6-BA) and 3% sucrosee0
- Outcome
- Callus could be obtainede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus could be obtained from immature seeds on MS solid medium augmented with 2.0 mg·L-16-benzylaminopurine (6-BA) and 3% sucrose.”
t0“Emmenopterys henryi”
Claim · model-written summary
Embryogenic calli were induced on MS basal medium with NAA and 6-BA.
- Taxon
- Emmenopterys henryiEmmenopterys henryit0
- Treatment
- MS basal medium supplemented with 0.1 mg·L-1 N-acetyl aspartate (NAA) and 0.5 mg ·L-16-BAe0
- Outcome
- Embryogenic calli were inducede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Embryogenic calli were induced on MS basal medium supplemented with 0.1 mg·L-1 N-acetyl aspartate (NAA) and 0.5 mg ·L-16-BA.”
t0“Emmenopterys henryi”
Claim · model-written summary
Numerous somatic embryoids appeared on liquid medium with NAA and 6-BA.
- Taxon
- Emmenopterys henryiEmmenopterys henryit0
- Treatment
- MS liquid basal nutrient medium with 0.5 mg·L-1 NAA and 0.5 mg·L-16-BAe0
- Outcome
- Numerous somatic embryoids appearede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Numerous somatic embryoids appeared on MS liquid basal nutrient medium with 0.5 mg·L-1 NAA and 0.5 mg·L-16-BA.”
t0“Emmenopterys henryi”
Claim · model-written summary
The optimal initial cell density was 2.0% fresh weight.
- Taxon
- Emmenopterys henryiEmmenopterys henryit0
- Outcome
- optimal value of the initial cell densitye0
- Value
- 2.0% (fresh weight)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The optimal value of the initial cell density was 2.0% (fresh weight).”
t0“Emmenopterys henryi”
Claim · model-written summary
Embryo-derived plantlets with roots and shoots transferred successfully to the greenhouse.
- Taxon
- Emmenopterys henryiEmmenopterys henryit0
- Organ / stage
- embryo-derived plantletse0
- Outcome
- transferred successfullye0
- Setting
- greenhousee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The embryo-derived plantlets with well-developed roots and shoots were transferred successfully to the greenhouse, with a high survival rate of 95%.”
t0“Emmenopterys henryi”
Claim · model-written summary
Greenhouse survival of embryo-derived plantlets was 95%.
- Taxon
- Emmenopterys henryiEmmenopterys henryit0
- Organ / stage
- embryo-derived plantletse0
- Outcome
- survival ratee0
- Value
- 95%e0
- Setting
- greenhousee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The embryo-derived plantlets with well-developed roots and shoots were transferred successfully to the greenhouse, with a high survival rate of 95%.”
t0“Emmenopterys henryi”