Abstract
Manganese (Mn)-rich seedling cultivation may reduce cadmium (Cd) uptake in rice; however, its stage-dependent effects and underlying mechanisms remain unclear. Mn-rich seedlings were cultivated in a hydroponic system. Biomass, Cd and Mn accumulation, and transcriptomic profiles were subsequently assessed at different growth stages. The results demonstrated that Mn enrichment increased Mn accumulation in the seedlings by 18.9-76.0-fold. On day 26 after transplantation, Cd concentrations in roots and shoots were reduced by 79.9% and 89.2%, respectively, in Mn-rich seedlings. However, this effect reversed at later stages, resulting in a 1.4- fold increase in Cd concentration in brown rice grains. Cd fluxes in the phloem and xylem decreased by 37.3%-49.4% on day 26, but increased by 1.10-2.74- fold on day 120 after the seedlings were enriched with Mn. Transcriptomic analysis was performed to elucidate the molecular mechanisms underlying the temporal dynamics of cadmium flux in rice tissues. Early-stage upregulation of genes such as Osj_27264, HSP17.4, and DREB1B was associated with enhanced Cd immobilization in roots, whereas the subsequent downregulation of key transcription factors including WRKY24, WRKY28, BHLH148, and TIFY11E in roots and nodes was associated with increased Cd uptake and translocation. Thus, the effect of Mn enrichment in rice seedlings on Cd uptake clearly indicates a time-dependent pattern. These findings suggest that elevated Mn levels should be maintained during the late reproductive stage to maximize Cd reduction in rice grains.
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Claims 10
Claim · model-written summary
Mn enrichment increased seedling Mn accumulation by 18.9-76.0-fold.
- Taxon
- rice
Oryza sativat0 - Treatment
- Mn enrichmente0
- Outcome
- Mn accumulation in the seedlingse0
- Direction
- increasede0
- Value
- 18.9-76.0-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mn enrichment increased Mn accumulation in the seedlings by 18.9-76.0-fold.”
t0“rice seedlings”
Claim · model-written summary
On day 26, Mn-rich seedlings had 79.9% lower root Cd concentration.
- Taxon
- riceOryza sativat0
- Treatment
- Mn-rich seedlingse0
- Outcome
- Cd concentrations in rootse0
- Direction
- reducede0
- Value
- 79.9%e0
- Qualifier
- day 26 after transplantatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“On day 26 after transplantation, Cd concentrations in roots and shoots were reduced by 79.9% and 89.2%, respectively, in Mn-rich seedlings.”
t0“rice seedlings”
Claim · model-written summary
On day 26, Mn-rich seedlings had 89.2% lower shoot Cd concentration.
- Taxon
- riceOryza sativat0
- Treatment
- Mn-rich seedlingse0
- Outcome
- Cd concentrations in roots and shootse0
- Direction
- reducede0
- Value
- 89.2%e0
- Qualifier
- day 26 after transplantatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“On day 26 after transplantation, Cd concentrations in roots and shoots were reduced by 79.9% and 89.2%, respectively, in Mn-rich seedlings.”
t0“rice seedlings”
Claim · model-written summary
Later, Cd concentration in brown rice grains rose 1.4-fold.
- Taxon
- riceOryza sativae0
- Treatment
- Mn-rich seedlingse1
- Outcome
- Cd concentration in brown rice grainse0
- Direction
- increasee0
- Value
- 1.4- folde0
- Qualifier
- at later stagese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this effect reversed at later stages, resulting in a 1.4- fold increase in Cd concentration in brown rice grains.”
e1“in Mn-rich seedlings.”
Claim · model-written summary
Mn enrichment decreased phloem Cd flux by 37.3%-49.4% on day 26.
- Taxon
- riceOryza sativat0
- Treatment
- Mn enrichmente1
- Outcome
- Cd fluxes in the phloeme0
- Direction
- decreasede0
- Value
- 37.3%-49.4%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cd fluxes in the phloem and xylem decreased by 37.3%-49.4% on day 26”
e1“Mn enrichment increased Mn accumulation in the seedlings by 18.9-76.0-fold.”
t0“rice seedlings”
t1“Manganese enrichment”
Claim · model-written summary
Mn enrichment decreased xylem Cd flux by 37.3%-49.4% on day 26.
- Taxon
- riceOryza sativat0
- Treatment
- Mn enrichmente1
- Outcome
- Cd fluxes in the phloem and xyleme0
- Direction
- decreasede0
- Value
- 37.3%-49.4%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cd fluxes in the phloem and xylem decreased by 37.3%-49.4% on day 26”
e1“Mn enrichment increased Mn accumulation in the seedlings by 18.9-76.0-fold.”
t0“rice seedlings”
t1“Manganese enrichment”
Claim · model-written summary
Mn enrichment increased phloem Cd flux 1.10-2.74-fold on day 120.
- Taxon
- riceOryza sativat0
- Treatment
- enriched with Mne0
- Outcome
- Cd fluxes in the phloem and xyleme1
- Direction
- increasede0
- Value
- 1.10-2.74- folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased by 1.10-2.74- fold on day 120 after the seedlings were enriched with Mn.”
e1“Cd fluxes in the phloem and xylem”
t0“rice seedlings”
Claim · model-written summary
Early upregulation of Osj_27264, HSP17.4 and DREB1B was associated with root Cd immobilization.
- Taxon
- riceOryza sativat0
- Gene
- Osj_27264, HSP17.4, and DREB1Be0
- Outcome
- Cd immobilization in rootse0
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Early-stage upregulation of genes such as Osj_27264, HSP17.4, and DREB1B was associated with enhanced Cd immobilization in roots”
t0“rice seedlings”
Claim · model-written summary
Later downregulation of WRKY24, WRKY28, BHLH148 and TIFY11E in roots and nodes was associated with increased Cd uptake and translocation.
- Taxon
- riceOryza sativat0
- Organ / stage
- roots and nodese0
- Gene
- WRKY24, WRKY28, BHLH148, and TIFY11Ee0
- Outcome
- Cd uptake and translocatione0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“subsequent downregulation of key transcription factors including WRKY24, WRKY28, BHLH148, and TIFY11E in roots and nodes was associated with increased Cd uptake and translocation.”
t0“rice seedlings”
Claim · model-written summary
The authors suggest maintaining elevated Mn late in reproduction to reduce grain Cd.
- Taxon
- riceOryza sativae0
- Organ / stage
- late reproductive stagee0
- Outcome
- elevated Mn levels should be maintainede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“elevated Mn levels should be maintained during the late reproductive stage to maximize Cd reduction in rice grains.”
The same questions in other papers
manganese atom · nutrient: content · Oryza sativa (rice)Positive and negative results
22 papers in the same direction as this paper · 8 in the opposite direction · 1 reporting no difference from an alternative
- positive
- positive
- positive
28 papers more in this group
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