Abstract
The development of crop cultivars with less Cd uptake in roots and accumulation in shoots is a most efficient and environment-friendly approach to deal with soil Cd contamination. Recently repression of Nramp5 expression or its knockout is commonly recognized to be efficient for reducing Cd accumulation in plants, but such mutant plants suffer from manganese deficiency. In this study, we assessed the efficacy of exogenous Mn addition in mitigating Cd stress in a japonica rice cultivar Xidao 1 (Wild Type, WT) and its OsNramp5 knockout mutant. Exposure to Cd stress resulted in notable low photosynthetic rate, growth inhibition, and high Cd accumulation in rice seedlings. Although the mutant plants contained much lower Cd concentration in both roots and shoots than the WT plants, their growth was significantly inhibited relative to the WT plants under the normal condition. Exogenous application of Mn (40 μM) dramatically reduces root and shoot Cd concentrations and alleviates the toxic effect of Cd stress in both rice types, with the mutant plants demonstrating lower Cd concentration and less Cd toxicity in comparison with WT plants. The alleviation of Cd toxicity by Mn addition was more effective in higher Cd level (1.0 μM) than in lower Cd level (0.1 μM). Mn increases the expression of OsNramp5 and other genes, including OsHMA2, OsHMA3, OsIRT1, and OsIRT2, which encode ion transporters related to Mn uptake and transportation, and meanwhile reduces Cd uptake and accumulation in rice seedlings. In short, the knockout of OsNramp5 results in the significant reduction of Cd uptake, but accompanies with Mn deficiency in rice plants, which can be efficiently overcome through exogenous Mn addition.
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Claims 15
Claim · model-written summary
Cd stress resulted in a low photosynthetic rate in rice seedlings.
- Taxon
- rice
Oryza sativat0 - Treatment
- Cd stresse1
- Outcome
- photosynthetic ratee1
- Direction
- lowe1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sufficient manganese supply is necessary for OsNramp5 knockout rice plants”
e1“Exposure to Cd stress resulted in notable low photosynthetic rate, growth inhibition, and high Cd accumulation in rice seedlings”
Claim · model-written summary
Cd stress resulted in growth inhibition in rice seedlings.
- Taxon
- riceOryza sativat0
- Treatment
- Cd stresse1
- Outcome
- growthe1
- Direction
- inhibitione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sufficient manganese supply is necessary for OsNramp5 knockout rice plants”
e1“Exposure to Cd stress resulted in notable low photosynthetic rate, growth inhibition, and high Cd accumulation in rice seedlings”
Claim · model-written summary
Cd stress resulted in high Cd accumulation in rice seedlings.
- Taxon
- riceOryza sativat0
- Treatment
- Cd stresse1
- Outcome
- Cd accumulatione1
- Direction
- highe1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sufficient manganese supply is necessary for OsNramp5 knockout rice plants”
e1“Exposure to Cd stress resulted in notable low photosynthetic rate, growth inhibition, and high Cd accumulation in rice seedlings”
Claim · model-written summary
The OsNramp5 knockout mutant contained much lower Cd concentration in roots and shoots than WT rice under the stated conditions.
- Taxon
- riceOryza sativat0
- Material
- mutant plantse2
- Outcome
- Cd concentratione2
- Direction
- lowere2
- Comparator
- WT plantse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sufficient manganese supply is necessary for OsNramp5 knockout rice plants”
e2“Although the mutant plants contained much lower Cd concentration in both roots and shoots than the WT plants, their growth was significantly inhibited relative to the WT plants under the normal condition”
Claim · model-written summary
The OsNramp5 knockout mutant grew significantly less than WT under normal conditions.
- Taxon
- riceOryza sativat0
- Material
- mutant plantse2
- Outcome
- growthe2
- Direction
- inhibitede2
- Qualifier
- under the normal conditione2
- Comparator
- WT plantse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sufficient manganese supply is necessary for OsNramp5 knockout rice plants”
e2“Although the mutant plants contained much lower Cd concentration in both roots and shoots than the WT plants, their growth was significantly inhibited relative to the WT plants under the normal condition”
Claim · model-written summary
Exogenous Mn at 40 µM reduced root and shoot Cd concentrations in rice.
- Taxon
- riceOryza sativat0
- Treatment
- Exogenous application of Mn (40 μM)e3
- Outcome
- root and shoot Cd concentrationse3
- Direction
- reducese3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sufficient manganese supply is necessary for OsNramp5 knockout rice plants”
e3“Exogenous application of Mn (40 μM) dramatically reduces root and shoot Cd concentrations and alleviates the toxic effect of Cd stress in both rice types”
Claim · model-written summary
Exogenous Mn at 40 µM alleviated the toxic effect of Cd stress in both rice types.
- Taxon
- riceOryza sativat0
- Treatment
- Exogenous application of Mn (40 μM)e3
- Outcome
- toxic effect of Cd stresse3
- Direction
- alleviatese3
- Qualifier
- in both rice typese3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sufficient manganese supply is necessary for OsNramp5 knockout rice plants”
e3“Exogenous application of Mn (40 μM) dramatically reduces root and shoot Cd concentrations and alleviates the toxic effect of Cd stress in both rice types”
Claim · model-written summary
The mutant had lower Cd toxicity than WT rice.
- Taxon
- riceOryza sativat0
- Material
- mutant plantse4
- Outcome
- Cd toxicitye4
- Direction
- lesse4
- Comparator
- WT plantse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sufficient manganese supply is necessary for OsNramp5 knockout rice plants”
e4“with the mutant plants demonstrating lower Cd concentration and less Cd toxicity in comparison with WT plants”
Claim · model-written summary
Mn addition was more effective at reducing Cd toxicity at the higher Cd level (1.0 µM) than at the lower level (0.1 µM).
- Taxon
- riceOryza sativat0
- Treatment
- higher Cd level (1.0 μM)e5
- Outcome
- alleviation of Cd toxicitye5
- Direction
- more effectivee5
- Comparator
- lower Cd level (0.1 μM)e5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sufficient manganese supply is necessary for OsNramp5 knockout rice plants”
e5“The alleviation of Cd toxicity by Mn addition was more effective in higher Cd level (1.0 μM) than in lower Cd level (0.1 μM)”
Claim · model-written summary
Mn increased the expression of OsNramp5 in rice.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sufficient manganese supply is necessary for OsNramp5 knockout rice plants”
e6“Mn increases the expression of OsNramp5 and other genes, including OsHMA2, OsHMA3, OsIRT1, and OsIRT2, which encode ion transporters related to Mn uptake and transportation”
Claim · model-written summary
Mn increased the expression of OsHMA2 and OsHMA3 in rice.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sufficient manganese supply is necessary for OsNramp5 knockout rice plants”
e6“Mn increases the expression of OsNramp5 and other genes, including OsHMA2, OsHMA3, OsIRT1, and OsIRT2, which encode ion transporters related to Mn uptake and transportation”
Claim · model-written summary
Mn increased the expression of OsIRT1 and OsIRT2 in rice.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sufficient manganese supply is necessary for OsNramp5 knockout rice plants”
e6“Mn increases the expression of OsNramp5 and other genes, including OsHMA2, OsHMA3, OsIRT1, and OsIRT2, which encode ion transporters related to Mn uptake and transportation”
Claim · model-written summary
The OsNramp5 knockout significantly reduced Cd uptake in rice.
- Taxon
- riceOryza sativat0
- Treatment
- knockout of OsNramp5e7
- Outcome
- Cd uptakee7
- Direction
- reductione7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sufficient manganese supply is necessary for OsNramp5 knockout rice plants”
e7“the knockout of OsNramp5 results in the significant reduction of Cd uptake, but accompanies with Mn deficiency in rice plants”
Claim · model-written summary
The OsNramp5 knockout rice accompanies Mn deficiency.
- Taxon
- riceOryza sativat0
- Treatment
- knockout of OsNramp5e7
- Outcome
- Mn deficiencye7
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sufficient manganese supply is necessary for OsNramp5 knockout rice plants”
e7“the knockout of OsNramp5 results in the significant reduction of Cd uptake, but accompanies with Mn deficiency in rice plants”
Claim · model-written summary
Mn deficiency in OsNramp5 knockout rice can be efficiently overcome through exogenous Mn addition.
- Taxon
- riceOryza sativat0
- Treatment
- exogenous Mn additione1
- Outcome
- Mn deficiencye7
- Direction
- overcomee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sufficient manganese supply is necessary for OsNramp5 knockout rice plants”
e7“the knockout of OsNramp5 results in the significant reduction of Cd uptake, but accompanies with Mn deficiency in rice plants”
e1“which can be efficiently overcome through exogenous Mn addition”
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