Paper · 2026 · Crop Protection

Biological activity of pomegranate peel for the green management of Ephestia kuehniella (Lepidoptera: Pyralidae) larvae

Federica Lo Muzio; Onofrio Marco Pistillo; Ilaria D'Isita; et al.

8
claims
22
verbatim quotes
0
citations
2
research areas
✓
Open access · cc-by

Abstract

The biological activity of pomegranate peel extracts (acetone, n -hexane, and diethyl ether) against larvae of Ephestia kuehniella was evaluated through contact, ingestion, and repellency bioassays. In topical application assays, the acetone extract exhibited the highest toxicity, with LD 50 values of 140.52 and 131.27 μg/larva after 24 and 48 h, respectively. The n -hexane extract displayed intermediate activity, whereas the diethyl ether extract was less effective. In flour disk ingestion assays, the acetone extract significantly impaired larval performance at 750 μg/disk, inducing pronounced feeding deterrence (FDI: 79.99%) and marked reductions in relative growth and consumption rates. In filter paper area preference assays, the acetone extract reached 80% repellency shortly after exposure, compared with lower responses recorded for the n -hexane and diethyl ether extracts. Overall, the acetone extract of pomegranate peel, characterized by the predominance of sitosterols, 9,12-octadecadienoic acid, and n-hexadecanoic acid, represents a promising candidate for the development of plant-based strategies in stored-product protection.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 8

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Acetone extract of pomegranate peel had the highest topical toxicity to Ephestia kuehniella larvae, with LD 50 values of 140.52 and 131.27 μg/larva at 24 and 48 h.

Taxon
pomegranatePunica granatumt0
Material
acetone extracte1
Interactor
Ephestia kuehniellaEphestia kuehniellae0pest
Outcome
LD 50e1
Value
140.52 and 131.27 μg/larvae1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗
Ephestia kuehniella
Ephestia kuehniella · InteractorBirgit E. Rhode, Landcare Research New Zealand Ltd. · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

t0

“pomegranate peel”

✓ located verbatim in the source · title, characters 23–39 · show in text ↑
e0

“The biological activity of pomegranate peel extracts (acetone, n -hexane, and diethyl ether) against larvae of Ephestia kuehniella was evaluated through contact, ingestion, and repellency bioassays”

✓ located verbatim in the source · abstract, characters 0–197 · show in text ↑
e1

“In topical application assays, the acetone extract exhibited the highest toxicity, with LD 50 values of 140.52 and 131.27 μg/larva after 24 and 48 h, respectively”

✓ located verbatim in the source · abstract, characters 199–361 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

The n-hexane extract of pomegranate peel had intermediate activity against Ephestia kuehniella larvae.

Taxon
pomegranatePunica granatumt0
Material
n -hexane extracte2
Interactor
Ephestia kuehniellaEphestia kuehniellae0pest
Outcome
activitye2
Qualifier
intermediatee2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗
Ephestia kuehniella
Ephestia kuehniella · InteractorBirgit E. Rhode, Landcare Research New Zealand Ltd. · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

t0

“pomegranate peel”

✓ located verbatim in the source · title, characters 23–39 · show in text ↑
e0

“The biological activity of pomegranate peel extracts (acetone, n -hexane, and diethyl ether) against larvae of Ephestia kuehniella was evaluated through contact, ingestion, and repellency bioassays”

✓ located verbatim in the source · abstract, characters 0–197 · show in text ↑
e2

“The n -hexane extract displayed intermediate activity, whereas the diethyl ether extract was less effective”

✓ located verbatim in the source · abstract, characters 363–470 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Acetone extract at 750 μg/disk significantly impaired Ephestia kuehniella larval performance in the ingestion assay.

Taxon
pomegranatePunica granatumt0
Treatment
acetone extracte3
Interactor
Ephestia kuehniellaEphestia kuehniellae0pest
Outcome
larval performancee3
Direction
impairede3
Value
750 μg/diske3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗
Ephestia kuehniella
Ephestia kuehniella · InteractorBirgit E. Rhode, Landcare Research New Zealand Ltd. · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

t0

“pomegranate peel”

✓ located verbatim in the source · title, characters 23–39 · show in text ↑
e0

“The biological activity of pomegranate peel extracts (acetone, n -hexane, and diethyl ether) against larvae of Ephestia kuehniella was evaluated through contact, ingestion, and repellency bioassays”

✓ located verbatim in the source · abstract, characters 0–197 · show in text ↑
e3

“the acetone extract significantly impaired larval performance at 750 μg/disk, inducing pronounced feeding deterrence (FDI: 79.99%) and marked reductions in relative growth and consumption rates”

✓ located verbatim in the source · abstract, characters 504–697 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Acetone extract at 750 μg/disk produced feeding deterrence of 79.99% in Ephestia kuehniella larvae.

Taxon
pomegranatePunica granatumt0
Material
acetone extracte3
Interactor
Ephestia kuehniellaEphestia kuehniellae0pest
Outcome
feeding deterrencee3
Value
FDI: 79.99%e3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗
Ephestia kuehniella
Ephestia kuehniella · InteractorBirgit E. Rhode, Landcare Research New Zealand Ltd. · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

t0

“pomegranate peel”

✓ located verbatim in the source · title, characters 23–39 · show in text ↑
e0

“The biological activity of pomegranate peel extracts (acetone, n -hexane, and diethyl ether) against larvae of Ephestia kuehniella was evaluated through contact, ingestion, and repellency bioassays”

✓ located verbatim in the source · abstract, characters 0–197 · show in text ↑
e3

“the acetone extract significantly impaired larval performance at 750 μg/disk, inducing pronounced feeding deterrence (FDI: 79.99%) and marked reductions in relative growth and consumption rates”

✓ located verbatim in the source · abstract, characters 504–697 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Acetone extract at 750 μg/disk markedly reduced relative growth and consumption rates of Ephestia kuehniella larvae.

Taxon
pomegranatePunica granatumt0
Treatment
acetone extracte3
Interactor
Ephestia kuehniellaEphestia kuehniellae0pest
Outcome
relative growth and consumption ratese3
Direction
marked reductionse3
Value
750 μg/diske3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗
Ephestia kuehniella
Ephestia kuehniella · InteractorBirgit E. Rhode, Landcare Research New Zealand Ltd. · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

t0

“pomegranate peel”

✓ located verbatim in the source · title, characters 23–39 · show in text ↑
e0

“The biological activity of pomegranate peel extracts (acetone, n -hexane, and diethyl ether) against larvae of Ephestia kuehniella was evaluated through contact, ingestion, and repellency bioassays”

✓ located verbatim in the source · abstract, characters 0–197 · show in text ↑
e3

“the acetone extract significantly impaired larval performance at 750 μg/disk, inducing pronounced feeding deterrence (FDI: 79.99%) and marked reductions in relative growth and consumption rates”

✓ located verbatim in the source · abstract, characters 504–697 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Acetone extract reached 80% repellency shortly after exposure, higher than the n-hexane and diethyl ether extracts.

Taxon
pomegranatePunica granatumt0
Material
acetone extracte4
Interactor
Ephestia kuehniellaEphestia kuehniellae0pest
Outcome
repellencye4
Value
80%e4
Comparator
n -hexane and diethyl ether extractse4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗
Ephestia kuehniella
Ephestia kuehniella · InteractorBirgit E. Rhode, Landcare Research New Zealand Ltd. · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

t0

“pomegranate peel”

✓ located verbatim in the source · title, characters 23–39 · show in text ↑
e0

“The biological activity of pomegranate peel extracts (acetone, n -hexane, and diethyl ether) against larvae of Ephestia kuehniella was evaluated through contact, ingestion, and repellency bioassays”

✓ located verbatim in the source · abstract, characters 0–197 · show in text ↑
e4

“the acetone extract reached 80% repellency shortly after exposure, compared with lower responses recorded for the n -hexane and diethyl ether extracts”

✓ located verbatim in the source · abstract, characters 739–889 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The acetone extract of pomegranate peel is characterised by a predominance of sitosterols, 9,12-octadecadienoic acid and n-hexadecanoic acid.

Taxon
pomegranatePunica granatumt0
Material
acetone extracte5
Outcome
predominance of sitosterols, 9,12-octadecadienoic acid, and n-hexadecanoic acide5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“pomegranate peel”

✓ located verbatim in the source · title, characters 23–39 · show in text ↑
e5

“the acetone extract of pomegranate peel, characterized by the predominance of sitosterols, 9,12-octadecadienoic acid, and n-hexadecanoic acid, represents a promising candidate for the development of plant-based strategies in stored-product protection”

✓ located verbatim in the source · abstract, characters 900–1150 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The acetone extract is a promising candidate for plant-based strategies in stored-product protection.

Taxon
pomegranatePunica granatumt0
Outcome
promising candidate for the development of plant-based strategies in stored-product protectione5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“pomegranate peel”

✓ located verbatim in the source · title, characters 23–39 · show in text ↑
e5

“the acetone extract of pomegranate peel, characterized by the predominance of sitosterols, 9,12-octadecadienoic acid, and n-hexadecanoic acid, represents a promising candidate for the development of plant-based strategies in stored-product protection”

✓ located verbatim in the source · abstract, characters 900–1150 · show in text ↑