Abstract
Post-harvest senescence and fungal infection, particularly Pink Mold Rot caused by Trichothecium roseum , significantly compromise the storage quality and economic value of muskmelon ( Cucumis melo cv. Yujinxiang). While Silicon (Si) application is recognized as an effective strategy for enhancing post-harvest disease resistance, the molecular mechanisms driving this resistance—specifically the interplay between DNA methylation, transcriptional regulation, and mitochondrial function—remain largely uncharacterized. This study employed an integrated multi-omics approach, combining Whole-Genome Bisulfite Sequencing (WGBS), RNA sequencing (RNA- seq ), and physiological assays, to elucidate the regulatory networks induced by Si treatment in T. roseum-inoculated muskmelons. Physiologically, Si treatment significantly alleviated oxidative stress and maintained mitochondrial energy status, evidenced by elevated ATP levels and increased Ca 2+ -ATPase activity compared to untreated controls. At the epigenomic level, we identified a distinct, context-specific enhancement of DNA methylation induced by Si. Notably, the CG context within the upstream 2 kilobases (up2k) regions exhibited the most significant response to treatment, surpassing changes in CHG and CHH contexts. Integrative analysis of the methylome and transcriptome revealed that this observed promoter hypermethylation correlates with the fine-tuning of gene expression related to energy metabolism and defense. Rather than simple repression, this epigenetic modification appears to stabilize transcriptional responses, potentially preventing the metabolic energy drain associated with hyper-immune responses or delaying the activation of senescence-associated genes. These findings establish a novel mechanistic link between epigenetic modification, and cellular energy metabolism. Ultimately, this research provides a theoretical basis for the utilization of Si as a post-harvest treatment to extend the shelf life of muskmelons by reinforcing mitochondrial function via epigenetic regulation.
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Claims 7
Claim · model-written summary
Si treatment alleviates oxidative stress in muskmelon compared with untreated controls.
- Taxon
- muskmelon
.jpg?width=60)
Cucumis melot0 - Material
- Yujinxiange0
- Treatment
- Si treatmente1
- Outcome
- oxidative stresse1
- Direction
- alleviatede1
- Comparator
- untreated controlse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“Multi-omics analysis of the role of muskmelon mitochondria in Si-induced resistance mechanisms”
e0“Cucumis melo cv. Yujinxiang”
e1“Physiologically, Si treatment significantly alleviated oxidative stress and maintained mitochondrial energy status”
e2“evidenced by elevated ATP levels and increased Ca 2+ -ATPase activity compared to untreated controls”
Claim · model-written summary
Si treatment elevates ATP levels in muskmelon compared with untreated controls.
- Taxon
- muskmelonCucumis melot0
- Material
- Yujinxiange0
- Treatment
- Si treatmente1
- Outcome
- ATP levelse2
- Direction
- elevatede2
- Comparator
- untreated controlse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Multi-omics analysis of the role of muskmelon mitochondria in Si-induced resistance mechanisms”
e0“Cucumis melo cv. Yujinxiang”
e1“Physiologically, Si treatment significantly alleviated oxidative stress and maintained mitochondrial energy status”
e2“evidenced by elevated ATP levels and increased Ca 2+ -ATPase activity compared to untreated controls”
Claim · model-written summary
Si treatment increases Ca 2+ -ATPase activity in muskmelon compared with untreated controls.
- Taxon
- muskmelonCucumis melot0
- Material
- Yujinxiange0
- Treatment
- Si treatmente1
- Outcome
- Ca 2+ -ATPase activitye2
- Direction
- increasede2
- Comparator
- untreated controlse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Multi-omics analysis of the role of muskmelon mitochondria in Si-induced resistance mechanisms”
e0“Cucumis melo cv. Yujinxiang”
e1“Physiologically, Si treatment significantly alleviated oxidative stress and maintained mitochondrial energy status”
e2“evidenced by elevated ATP levels and increased Ca 2+ -ATPase activity compared to untreated controls”
Claim · model-written summary
Si induces an enhancement of DNA methylation in muskmelon.
- Taxon
- muskmelonCucumis melot0
- Treatment
- Sie4
- Outcome
- DNA methylatione4
- Direction
- enhancemente4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Multi-omics analysis of the role of muskmelon mitochondria in Si-induced resistance mechanisms”
e4“we identified a distinct, context-specific enhancement of DNA methylation induced by Si”
Claim · model-written summary
The CG context in the upstream 2 kb regions responds most strongly to Si treatment, more than the CHG and CHH contexts.
- Taxon
- muskmelonCucumis melot0
- Treatment
- treatmente5
- Outcome
- CG contexte5
- Direction
- surpassinge5
- Comparator
- CHG and CHH contextse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Multi-omics analysis of the role of muskmelon mitochondria in Si-induced resistance mechanisms”
e5“the CG context within the upstream 2 kilobases (up2k) regions exhibited the most significant response to treatment, surpassing changes in CHG and CHH contexts”
Claim · model-written summary
Promoter hypermethylation correlates with fine-tuned expression of genes related to energy metabolism and defense.
- Taxon
- muskmelonCucumis melot0
- Treatment
- promoter hypermethylatione6
- Outcome
- gene expression related to energy metabolism and defensee6
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Multi-omics analysis of the role of muskmelon mitochondria in Si-induced resistance mechanisms”
e6“this observed promoter hypermethylation correlates with the fine-tuning of gene expression related to energy metabolism and defense”
Claim · model-written summary
The authors propose Si as a post-harvest treatment to extend the shelf life of muskmelons.
- Taxon
- muskmelonsCucumis meloe7
- Outcome
- shelf lifee7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“this research provides a theoretical basis for the utilization of Si as a post-harvest treatment to extend the shelf life of muskmelons”
The same questions in other papers
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1 paper in the same direction as this paper
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.