Paper · 2026 · BMC Plant Biology

Nitrogen fertilization effects on biomass yield and silage quality of rainfed winter forage cereals in Pakistan

Muhammad Jamil; Raees Ahmed; Nilay Kayin; et al.

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verbatim quotes
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citations
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Open access · cc-by-nc-nd

Abstract

Optimizing nitrogen (N) fertilizer regimes in conjunction with harvesting stage (HS) (booting and milking dough stages) may potentially boost the herbage yield and silage nutritional quality of winter forage cereals (WFCs), particularly in rainfed agroecosystems. A two-season field study was executed to optimize the doses of N (0, 75, 150, and 225 kg ha − 1 ) for WFCs (barley, oat, rye, and triticale) harvested at booting stage (BS) and milking dough stage (MDS) and to improve silage quality using additives (molasses and formic acid applied at 5 kg ton − 1 of biomass). The trial was conducted using a randomized complete block design in a factorial arrangement. Oats produced the maximum yield contributing traits and 42% higher fresh biomass yield under the N regime of 225 kg ha − 1 than the control at BS. Interestingly, at MDS, oat outperformed the rest of the WFCs under 225 kg ha − 1 N, which remained statistically comparable to 150 kg ha − 1 . The minimum biomass yield was noted for rye under control conditions at both HS. The fertilizer use efficiency (FUE) was maximized across all WFCs at the lowest N dose, whereas oat consistently recorded the maximum FUE and water use efficiency (WUE) at both HS across seasons. Concerning the nutritional quality of silage, the maximum crude protein (CP) content was recorded for oat in response to the N 3 fertilization regime with additives, while triticale CP contents were equal at the same level of N and molasses, whereas rye recorded the minimum acid detergent fiber and neutral detergent fiber along with the highest relative feed value in response to the highest N dose. Oat may be recommended as a superior forage crop because of the highest biomass productivity, particularly under high nitrogen input (225 kg ha − 1 ). Moreover, oat harvested at BS may be supplemented with molasses as an additive to maximize the crude protein content of silage.

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Claims 13

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Oat under 225 kg N per ha produced 42% higher fresh biomass yield than the control at the booting stage.

Treatment
N regime of 225 kg ha − 1e0
Outcome
fresh biomass yielde0
Direction
highere0
Value
42%e0
Qualifier
BSe0
Comparator
the controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Avena sativa
Avena sativa · TaxonSendelbach ( talk ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Oats produced the maximum yield contributing traits and 42% higher fresh biomass yield under the N regime of 225 kg ha − 1 than the control at BS”

✓ located verbatim in the source · abstract, characters 669–814 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Oat produced the maximum yield contributing traits.

Outcome
maximum yield contributing traitse0
Direction
maximume0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Avena sativa
Avena sativa · TaxonSendelbach ( talk ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Oats produced the maximum yield contributing traits”

✓ located verbatim in the source · abstract, characters 669–720 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

At the milking dough stage oat outperformed the other winter forage cereals under 225 kg N per ha.

Treatment
225 kg ha − 1 Ne0
Outcome
outperformed the rest of the WFCse0
Qualifier
MDSe0
Comparator
the rest of the WFCse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Avena sativa
Avena sativa · TaxonSendelbach ( talk ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“at MDS, oat outperformed the rest of the WFCs under 225 kg ha − 1 N, which remained statistically comparable to 150 kg ha − 1”

✓ located verbatim in the source · abstract, characters 831–956 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

In oat at the milking dough stage, 225 kg N per ha remained statistically comparable to 150 kg N per ha.

Treatment
225 kg ha − 1 Ne0
Outcome
statistically comparablee0
Direction
statistically comparablee0
Qualifier
MDSe0
Comparator
150 kg ha − 1e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Avena sativa
Avena sativa · TaxonSendelbach ( talk ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“at MDS, oat outperformed the rest of the WFCs under 225 kg ha − 1 N, which remained statistically comparable to 150 kg ha − 1”

✓ located verbatim in the source · abstract, characters 831–956 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Rye under control conditions had the minimum biomass yield at both harvesting stages.

Treatment
control conditionse0
Outcome
minimum biomass yielde0
Qualifier
both HSe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Secale cereale
Secale cereale · TaxonLSDSL · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The minimum biomass yield was noted for rye under control conditions at both HS”

✓ located verbatim in the source · abstract, characters 959–1038 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Fertilizer use efficiency was maximized across all winter forage cereals at the lowest N dose.

Taxon
WFCse0
Treatment
lowest N dosee0
Outcome
fertilizer use efficiency (FUE)e0
Direction
maximizede0

Verbatim

e0

“The fertilizer use efficiency (FUE) was maximized across all WFCs at the lowest N dose”

✓ located verbatim in the source · abstract, characters 1040–1126 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Oat consistently recorded the maximum fertilizer use efficiency and water use efficiency at both harvesting stages across seasons.

Outcome
FUE and water use efficiency (WUE)e0
Direction
maximume0
Qualifier
both HS across seasonse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Avena sativa
Avena sativa · TaxonSendelbach ( talk ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“oat consistently recorded the maximum FUE and water use efficiency (WUE) at both HS across seasons”

✓ located verbatim in the source · abstract, characters 1136–1234 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Oat with the N3 fertilization regime plus additives had the maximum silage crude protein content.

Treatment
N 3 fertilization regime with additivese0
Outcome
maximum crude protein (CP) contente0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Avena sativa
Avena sativa · TaxonSendelbach ( talk ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the maximum crude protein (CP) content was recorded for oat in response to the N 3 fertilization regime with additives”

✓ located verbatim in the source · abstract, characters 1282–1400 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Triticale crude protein content was equal to that of oat at the same N level with molasses.

Taxon
triticale×Triticosecalee0
Treatment
same level of N and molassese0
Outcome
CP contents were equale0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

x Triticosecale
×Triticosecale · Taxon · picture from its Wikimedia Commons categoryMiquel Pujol Palol · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“triticale CP contents were equal at the same level of N and molasses”

✓ located verbatim in the source · abstract, characters 1408–1476 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Rye recorded the minimum acid detergent fiber and neutral detergent fiber in response to the highest N dose.

Treatment
highest N dosee0
Outcome
acid detergent fiber and neutral detergent fibere0
Direction
minimume0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Secale cereale
Secale cereale · TaxonLSDSL · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“rye recorded the minimum acid detergent fiber and neutral detergent fiber along with the highest relative feed value in response to the highest N dose”

✓ located verbatim in the source · abstract, characters 1486–1636 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Rye recorded the highest relative feed value in response to the highest N dose.

Treatment
highest N dosee0
Outcome
relative feed valuee0
Direction
higheste0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Secale cereale
Secale cereale · TaxonLSDSL · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“rye recorded the minimum acid detergent fiber and neutral detergent fiber along with the highest relative feed value in response to the highest N dose”

✓ located verbatim in the source · abstract, characters 1486–1636 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

Oat may be recommended as a superior forage crop because of its highest biomass productivity, particularly under high nitrogen input of 225 kg per ha.

Treatment
high nitrogen input (225 kg ha − 1 )e0
Outcome
superior forage crope0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Avena sativa
Avena sativa · TaxonSendelbach ( talk ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Oat may be recommended as a superior forage crop because of the highest biomass productivity, particularly under high nitrogen input (225 kg ha − 1 )”

✓ located verbatim in the source · abstract, characters 1638–1787 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

Oat harvested at the booting stage may be supplemented with molasses to maximize silage crude protein content.

Treatment
supplemented with molassese0
Outcome
maximize the crude protein content of silagee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Avena sativa
Avena sativa · TaxonSendelbach ( talk ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“oat harvested at BS may be supplemented with molasses as an additive to maximize the crude protein content of silage”

✓ located verbatim in the source · abstract, characters 1799–1915 · show in text ↑