Abstract
The pathogen Geotrichum citri-aurantii causes postharvest sour rot disease in citrus fruit worldwide, severely impacting the commercial development and economic value of citrus. However, research on G. citri-aurantii commenced relatively late, and the pathogenic mechanisms are incompletely understood. This study successfully constructed OE-GcPG2 # and OE-GcGel5 # overexpressing lines via PEG-mediated protoplast transformation. Compared to WT, the overexpressing strains enhanced pathogenicity by increasing chitin levels through the elevated expression of GcPG, GcGel, GcAGA, and GcCs . They promoted degradation of citrus protopectin, enhanced expression of key membrane lipid metabolism genes CitPLD and CitLOX, and increased malondialdehyde (MDA) accumulation, thereby compromising cell membrane integrity. This demonstrated that GcPG and GcGel represent critical targets for driving the progression of sour rot, filling gaps in our understanding of the pathogenic mechanisms of G. citri-aurantii . These findings hold significant implications for future drug target development and the advancement of integrated management strategies against sour rot.
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Claims 7
Claim · model-written summary
Overexpressing strains were more pathogenic to citrus fruit than wild type.
- Treatment
- overexpressing strainse1
- Interactor
- Geotrichum citri-aurantiiGeotrichum citri-aurantiie0pathogen
- Gene
- GcPG, GcGel, GcAGA, and GcCse1
- Outcome
- pathogenicitye1
- Direction
- enhancede1
- Comparator
- WTe1
CitrusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The pathogen Geotrichum citri-aurantii causes postharvest sour rot disease in citrus fruit worldwide”
e1“Compared to WT, the overexpressing strains enhanced pathogenicity by increasing chitin levels through the elevated expression of GcPG, GcGel, GcAGA, and GcCs”
Claim · model-written summary
Overexpressing strains increased chitin levels compared with wild type.
- Treatment
- overexpressing strainse1
- Interactor
- Geotrichum citri-aurantiiGeotrichum citri-aurantiie0pathogen
- Outcome
- chitin levelse1
- Direction
- increasinge1
- Comparator
- WTe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The pathogen Geotrichum citri-aurantii causes postharvest sour rot disease in citrus fruit worldwide”
e1“Compared to WT, the overexpressing strains enhanced pathogenicity by increasing chitin levels through the elevated expression of GcPG, GcGel, GcAGA, and GcCs”
Claim · model-written summary
Overexpressing strains promoted degradation of citrus protopectin compared with wild type.
- Treatment
- overexpressing strainse1
- Interactor
- Geotrichum citri-aurantiiGeotrichum citri-aurantiie0pathogen
- Outcome
- degradation of citrus protopectine2
- Direction
- promotede2
- Comparator
- WTe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The pathogen Geotrichum citri-aurantii causes postharvest sour rot disease in citrus fruit worldwide”
e1“Compared to WT, the overexpressing strains enhanced pathogenicity by increasing chitin levels through the elevated expression of GcPG, GcGel, GcAGA, and GcCs”
e2“They promoted degradation of citrus protopectin, enhanced expression of key membrane lipid metabolism genes CitPLD and CitLOX, and increased malondialdehyde (MDA) accumulation, thereby compromising cell membrane integrity”
Claim · model-written summary
Overexpressing strains enhanced expression of membrane lipid metabolism genes CitPLD and CitLOX compared with wild type.
- Treatment
- overexpressing strainse1
- Gene
- CitPLD and CitLOXe2
- Outcome
- expression of key membrane lipid metabolism genese2
- Direction
- enhancede2
- Comparator
- WTe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The pathogen Geotrichum citri-aurantii causes postharvest sour rot disease in citrus fruit worldwide”
e1“Compared to WT, the overexpressing strains enhanced pathogenicity by increasing chitin levels through the elevated expression of GcPG, GcGel, GcAGA, and GcCs”
e2“They promoted degradation of citrus protopectin, enhanced expression of key membrane lipid metabolism genes CitPLD and CitLOX, and increased malondialdehyde (MDA) accumulation, thereby compromising cell membrane integrity”
Claim · model-written summary
Overexpressing strains increased malondialdehyde accumulation in citrus fruit compared with wild type.
- Treatment
- overexpressing strainse1
- Outcome
- malondialdehyde (MDA) accumulatione2
- Direction
- increasede2
- Comparator
- WTe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The pathogen Geotrichum citri-aurantii causes postharvest sour rot disease in citrus fruit worldwide”
e1“Compared to WT, the overexpressing strains enhanced pathogenicity by increasing chitin levels through the elevated expression of GcPG, GcGel, GcAGA, and GcCs”
e2“They promoted degradation of citrus protopectin, enhanced expression of key membrane lipid metabolism genes CitPLD and CitLOX, and increased malondialdehyde (MDA) accumulation, thereby compromising cell membrane integrity”
Claim · model-written summary
Overexpressing strains compromised cell membrane integrity in citrus fruit compared with wild type.
- Treatment
- overexpressing strainse1
- Outcome
- cell membrane integritye2
- Direction
- compromisinge2
- Comparator
- WTe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The pathogen Geotrichum citri-aurantii causes postharvest sour rot disease in citrus fruit worldwide”
e1“Compared to WT, the overexpressing strains enhanced pathogenicity by increasing chitin levels through the elevated expression of GcPG, GcGel, GcAGA, and GcCs”
e2“They promoted degradation of citrus protopectin, enhanced expression of key membrane lipid metabolism genes CitPLD and CitLOX, and increased malondialdehyde (MDA) accumulation, thereby compromising cell membrane integrity”
Claim · model-written summary
GcPG and GcGel are proposed as critical targets for driving sour rot progression in citrus.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The pathogen Geotrichum citri-aurantii causes postharvest sour rot disease in citrus fruit worldwide”
e3“This demonstrated that GcPG and GcGel represent critical targets for driving the progression of sour rot”