Abstract
BACKGROUND: Angelica dahurica var. formosana is a medicinal and edible plant. Higher abundance of Proteobacteria is an excellent characteristic of its rhizosphere bacterial community. Aspergillus niger ZJ-17 (AN17) significantly improved plant yield and quality while reducing fertilizer input application in both pot and field experiments. However, the impact of inoculants on resident soil bacteria directly determines their field application. Therefore, to systematically analyze how AN17 remodels host rhizosphere bacterial communities, we inoculated AN17 into the roots of A. dahurica var. formosana. The rhizosphere bacterial communities and root exudates were investigated at the harvest stage. Rhizosphere bacteria were isolated for in vitro experiments to elucidate the reasons for the changes in the rhizosphere bacterial community. RESULTS: AN17 promoted nutrient utilization and absorption in rhizosphere soil and increased the accumulation of IAA and JA in plant roots. In the microbiome, the relative abundance of rhizosphere Proteobacteria increased after inoculation. A total of 832 bacterial strains were isolated from the rhizosphere of the host for in vitro experiments. In in vitro experiments, AN17 induced the enrichment of Proteobacteria through microbial interactions and the modulation of host root exudates. These root exudates promoted the proliferation of bacterial genera with higher abundance and diversity. In the metabolome, host root activity increased following AN17 inoculation. According to the KEGG analysis of root exudates, AN17 upregulated microbial metabolism in diverse environments, the biosynthesis of alkaloids derived from the shikimate pathway and tryptophan metabolism. Correlation analysis and in vitro tests revealed that AN17 regulated the secretion of phenolic acids (4-chlorophenol, 2-oxoadipic acid, pyrogallol, and vanillic acid) from roots, which serve as crucial components driving the enrichment of Proteobacteria. In both plate and pot experiments, these bacteria promoted the growth of A. dahurica var. formosana and activated nutrient availability. CONCLUSIONS: We supplemented multiple growth-promoting strategies by which AN17 improves rhizosphere bacterial communities. Phenolic acids in root exudates were recognized during the stimulation of rhizosphere bacterial proliferation by AN17. The interaction relationships among plants, beneficial fungi and rhizobacteria were explored and revealed. This result provides a sustainable approach for rhizosphere bacterial optimization and chemical fertilizer reduction in agricultural production.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 12
Claim · model-written summary
Aspergillus niger ZJ-17 was previously reported to improve plant yield and quality while reducing fertilizer input in pot and field experiments.
- Taxon
- Angelica dahurica var. formosana
.jpg?width=60)
Angelica dahurica var. formosanat0 - Treatment
- Aspergillus niger ZJ-17 (AN17)e0
- Interactor
- Aspergillus niger ZJ-17
Aspergillus nigere0symbiont - Outcome
- plant yield and qualitye0
- Direction
- improvede0
- Setting
- pot and field experimentse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“Angelica dahurica var. formosana”
e0“Aspergillus niger ZJ-17 (AN17) significantly improved plant yield and quality while reducing fertilizer input application in both pot and field experiments”
Claim · model-written summary
AN17 promoted nutrient utilization and absorption in rhizosphere soil.
- Taxon
- Angelica dahurica var. formosanaAngelica dahurica var. formosanat0
- Treatment
- AN17e1
- Interactor
- AN17e1symbiont
- Outcome
- nutrient utilization and absorption in rhizosphere soile1
- Direction
- promotede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Angelica dahurica var. formosana”
e1“AN17 promoted nutrient utilization and absorption in rhizosphere soil and increased the accumulation of IAA and JA in plant roots”
Claim · model-written summary
AN17 increased the accumulation of IAA and JA in plant roots.
- Taxon
- Angelica dahurica var. formosanaAngelica dahurica var. formosanat0
- Organ / stage
- plant rootse1
- Treatment
- AN17e1
- Interactor
- AN17e1symbiont
- Outcome
- accumulation of IAA and JA in plant rootse1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Angelica dahurica var. formosana”
e1“AN17 promoted nutrient utilization and absorption in rhizosphere soil and increased the accumulation of IAA and JA in plant roots”
Claim · model-written summary
The relative abundance of rhizosphere Proteobacteria increased after AN17 inoculation.
- Taxon
- Angelica dahurica var. formosanaAngelica dahurica var. formosanat0
- Treatment
- inoculatione2
- Interactor
- ProteobacteriaPseudomonadotae2other
- Outcome
- relative abundance of rhizosphere Proteobacteriae2
- Direction
- increasede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Angelica dahurica var. formosana”
e2“the relative abundance of rhizosphere Proteobacteria increased after inoculation”
Claim · model-written summary
A total of 832 bacterial strains were isolated from the rhizosphere of the host.
- Taxon
- Angelica dahurica var. formosanaAngelica dahurica var. formosanat0
- Outcome
- bacterial strains were isolated from the rhizospheree3
- Value
- 832e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Angelica dahurica var. formosana”
e3“A total of 832 bacterial strains were isolated from the rhizosphere of the host”
Claim · model-written summary
In in vitro experiments, AN17 induced enrichment of Proteobacteria through microbial interactions and modulation of host root exudates.
- Taxon
- Angelica dahurica var. formosanaAngelica dahurica var. formosanat0
- Treatment
- AN17e4
- Interactor
- AN17e4symbiont
- Second interactor
- ProteobacteriaPseudomonadotae4other
- Outcome
- enrichment of Proteobacteriae4
- Qualifier
- microbial interactions and the modulation of host root exudatese4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Angelica dahurica var. formosana”
e4“AN17 induced the enrichment of Proteobacteria through microbial interactions and the modulation of host root exudates”
Claim · model-written summary
Root exudates promoted the proliferation of bacterial genera with higher abundance and diversity.
- Taxon
- Angelica dahurica var. formosanaAngelica dahurica var. formosanat0
- Treatment
- root exudatese5
- Outcome
- proliferation of bacterial genera with higher abundance and diversitye5
- Direction
- promotede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Angelica dahurica var. formosana”
e5“These root exudates promoted the proliferation of bacterial genera with higher abundance and diversity”
Claim · model-written summary
Host root activity increased following AN17 inoculation in the metabolome analysis.
- Taxon
- Angelica dahurica var. formosanaAngelica dahurica var. formosanat0
- Treatment
- AN17 inoculatione6
- Interactor
- AN17e6symbiont
- Outcome
- host root activitye6
- Direction
- increasede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Angelica dahurica var. formosana”
e6“host root activity increased following AN17 inoculation”
Claim · model-written summary
According to KEGG analysis of root exudates, AN17 upregulated microbial metabolism, biosynthesis of shikimate-derived alkaloids and tryptophan metabolism.
- Taxon
- Angelica dahurica var. formosanaAngelica dahurica var. formosanat0
- Treatment
- AN17e7
- Interactor
- AN17e7symbiont
- Outcome
- microbial metabolism in diverse environments, the biosynthesis of alkaloids derived from the shikimate pathway and tryptophan metabolisme7
- Direction
- upregulatede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Angelica dahurica var. formosana”
e7“AN17 upregulated microbial metabolism in diverse environments, the biosynthesis of alkaloids derived from the shikimate pathway and tryptophan metabolism”
Claim · model-written summary
AN17 regulated the secretion of phenolic acids from roots, which are crucial components driving the enrichment of Proteobacteria.
- Taxon
- Angelica dahurica var. formosanaAngelica dahurica var. formosanat0
- Organ / stage
- rootse0
- Treatment
- AN17e0
- Interactor
- AN17e0symbiont
- Second interactor
- ProteobacteriaPseudomonadotae0other
- Compound
- phenolic acids (4-chlorophenol, 2-oxoadipic acid, pyrogallol, and vanillic acid)e0
- Outcome
- enrichment of Proteobacteriae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Angelica dahurica var. formosana”
e0“AN17 regulated the secretion of phenolic acids (4-chlorophenol, 2-oxoadipic acid, pyrogallol, and vanillic acid) from roots, which serve as crucial components driving the enrichment of Proteobacteria”
Claim · model-written summary
In plate and pot experiments, the isolated bacteria promoted growth of A. dahurica var. formosana and activated nutrient availability.
- Taxon
- Angelica dahurica var. formosanaAngelica dahurica var. formosanat0
- Treatment
- these bacteriae1
- Interactor
- these bacteriae1symbiont
- Outcome
- growth of A. dahurica var. formosana and activated nutrient availabilitye1
- Direction
- promotede1
- Setting
- plate and pot experimentse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Angelica dahurica var. formosana”
e1“In both plate and pot experiments, these bacteria promoted the growth of A. dahurica var. formosana and activated nutrient availability”
Claim · model-written summary
The authors state the result offers a sustainable approach for rhizosphere bacterial optimization and chemical fertilizer reduction in agricultural production.
- Taxon
- Angelica dahurica var. formosanaAngelica dahurica var. formosanat0
- Outcome
- sustainable approach for rhizosphere bacterial optimization and chemical fertilizer reductione2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Angelica dahurica var. formosana”
e2“This result provides a sustainable approach for rhizosphere bacterial optimization and chemical fertilizer reduction in agricultural production”