Abstract
In spite of potential benefits of anther culture, low productivity of plant regeneration in some genotypes; e.g. tonsil and indica rice, is one of the major obstacles for practical use of anther culture. This study was conducted to improve cold shock method and carbohydrate source for increasing the efficiency of anther culture in rice. The most common carbon source, sucrose was replaced to maltose, which has two molecules of glucose. Maltose increased callus induction 1.4-to 1.8-fold higher in japonica rice, 3.2-to 11.6-fold in tongil types and 2.7-fold in indica rice IR50. Callus induction was increased from 0.2％ to 12.5％ in maltose medium compared to the medium supplemented with sucrose plus glucose in indica rice "Tetep". A simple procedure of vacuum packaging of panicles during cold shock treatment prolonged not only anther viability more than 15 days but also increased callus induction more than 2-fold compared to open-air storage (conventional method). Combining of above two methods, callus induction was increased 28 to 56％ in japonica, 13 to 33％ in tonsil type and 12 to 31％ in indica rice. Plant regeneration was increased 14 to 35％ in japonica, 10 to 20％ in tonsil and 4 to 15％ in indica rice, respectively.
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Claims 12
Claim · model-written summary
Maltose raised callus induction 1.4-to 1.8-fold in japonica rice.
- Taxon
- rice
Oryza sativae0 - Material
- japonica ricee0
- Treatment
- Maltosee0
- Outcome
- callus inductione0
- Direction
- increasede0
- Value
- 1.4-to 1.8-folde0
- Comparator
- sucrosee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maltose increased callus induction 1.4-to 1.8-fold higher in japonica rice”
e1“sucrose was replaced to maltose”
Claim · model-written summary
Maltose raised callus induction 3.2-to 11.6-fold in tongil types.
- Taxon
- RiceOryza sativat0
- Material
- tongil typese0
- Treatment
- Maltosee0
- Outcome
- callus inductione0
- Direction
- increasede0
- Value
- 3.2-to 11.6-folde0
- Comparator
- sucrosee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maltose increased callus induction 1.4-to 1.8-fold higher in japonica rice, 3.2-to 11.6-fold in tongil types”
e1“sucrose was replaced to maltose”
t0“Rice”
Claim · model-written summary
Maltose raised callus induction 2.7-fold in indica rice IR50.
- Taxon
- riceOryza sativae0
- Material
- indica rice IR50e0
- Treatment
- Maltosee0
- Outcome
- callus inductione0
- Direction
- increasede0
- Value
- 2.7-folde0
- Comparator
- sucrosee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maltose increased callus induction 1.4-to 1.8-fold higher in japonica rice, 3.2-to 11.6-fold in tongil types and 2.7-fold in indica rice IR50”
e1“sucrose was replaced to maltose”
Claim · model-written summary
Maltose raised Tetep callus induction from 0.2％ to 12.5％ versus sucrose plus glucose.
- Taxon
- riceOryza sativae0
- Material
- Tetepe0
- Treatment
- maltose mediume0
- Outcome
- Callus inductione0
- Direction
- increasede0
- Value
- 12.5％e0
- Qualifier
- 0.2％e0
- Comparator
- the medium supplemented with sucrose plus glucosee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus induction was increased from 0.2％ to 12.5％ in maltose medium compared to the medium supplemented with sucrose plus glucose in indica rice "Tetep".”
Claim · model-written summary
Vacuum packaging extended anther viability beyond 15 days.
- Taxon
- RiceOryza sativat0
- Organ / stage
- anthere0
- Treatment
- vacuum packaging of panicles during cold shock treatmente0
- Outcome
- anther viabilitye0
- Direction
- prolongede0
- Value
- more than 15 dayse0
- Comparator
- open-air storagee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“vacuum packaging of panicles during cold shock treatment prolonged not only anther viability more than 15 days but also increased callus induction more than 2-fold compared to open-air storage”
t0“Rice”
Claim · model-written summary
Vacuum packaging raised callus induction more than twofold against open-air storage.
- Taxon
- RiceOryza sativat0
- Treatment
- vacuum packaging of panicles during cold shock treatmente0
- Outcome
- callus inductione0
- Direction
- increasede0
- Value
- more than 2-folde0
- Comparator
- open-air storagee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“vacuum packaging of panicles during cold shock treatment prolonged not only anther viability more than 15 days but also increased callus induction more than 2-fold compared to open-air storage”
t0“Rice”
Claim · model-written summary
The combined methods raised callus induction from 28 to 56％ in japonica.
- Taxon
- RiceOryza sativat0
- Material
- japonicae0
- Treatment
- Combining of above two methodse0
- Outcome
- callus inductione0
- Direction
- increasede0
- Value
- 28 to 56％e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Combining of above two methods, callus induction was increased 28 to 56％ in japonica”
t0“Rice”
Claim · model-written summary
The combined methods raised callus induction from 13 to 33％ in tonsil type.
- Taxon
- RiceOryza sativat0
- Material
- tonsil typee0
- Treatment
- Combining of above two methodse0
- Outcome
- callus inductione0
- Direction
- increasede0
- Value
- 13 to 33％e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Combining of above two methods, callus induction was increased 28 to 56％ in japonica, 13 to 33％ in tonsil type”
t0“Rice”
Claim · model-written summary
The combined methods raised callus induction from 12 to 31％ in indica rice.
- Taxon
- RiceOryza sativat0
- Material
- indica ricee0
- Treatment
- Combining of above two methodse0
- Outcome
- callus inductione0
- Direction
- increasede0
- Value
- 12 to 31％e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Combining of above two methods, callus induction was increased 28 to 56％ in japonica, 13 to 33％ in tonsil type and 12 to 31％ in indica rice”
t0“Rice”
Claim · model-written summary
The combined methods raised plant regeneration from 14 to 35％ in japonica.
- Taxon
- RiceOryza sativat0
- Material
- japonicae0
- Treatment
- Combining of above two methodse1
- Outcome
- Plant regeneratione0
- Direction
- increasede0
- Value
- 14 to 35％e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Plant regeneration was increased 14 to 35％ in japonica”
e1“Combining of above two methods”
t0“Rice”
Claim · model-written summary
The combined methods raised plant regeneration from 10 to 20％ in tonsil.
- Taxon
- RiceOryza sativat0
- Material
- tonsile0
- Treatment
- Combining of above two methodse1
- Outcome
- Plant regeneratione0
- Direction
- increasede0
- Value
- 10 to 20％e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Plant regeneration was increased 14 to 35％ in japonica, 10 to 20％ in tonsil”
e1“Combining of above two methods”
t0“Rice”
Claim · model-written summary
The combined methods raised plant regeneration from 4 to 15％ in indica rice.
- Taxon
- RiceOryza sativat0
- Material
- indica ricee0
- Treatment
- Combining of above two methodse1
- Outcome
- Plant regeneratione0
- Direction
- increasede0
- Value
- 4 to 15％e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Plant regeneration was increased 14 to 35％ in japonica, 10 to 20％ in tonsil and 4 to 15％ in indica rice”
e1“Combining of above two methods”
t0“Rice”
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See all 165 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.