Abstract
Plastids have been isolated from germinating pea (Pisum sativum L.) roots by differential centrifugation and purified on Percoll gradients. Marker enzymes (NADPH: cytochrome c reductase, fumarase and fatty acid synthesis) indicate that greater than 50% of the plastids are recovered essentially free from mitochondrial and endoplasmic reticulum contamination. Fatty acids synthesized from ({sup 14}C)acetate by Percoll-purified plastids are primarily 16:0, 16:1 and 18:1. ({sup 14}C)Acetate-labelled fatty acids and ({sup 14}C)glycerol-3-phosphate are both readily incorporated into glycerolipid. Approximately 12% of the total activity for glycerolipid biosynthesis from glycerol-3-phosphate is recovered in the purified plastid fraction. Glycerolipids synthesized from these precursors are primarily TAG, DAG, PE, PG, PC, PI and PA. Acyl-CoA's also accumulate when acetate is the precursor.
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Claims 6
Claim · model-written summary
Greater than 50% of the plastids were recovered essentially free from mitochondrial and endoplasmic reticulum contamination.
- Taxon
- pea
Lathyrus oleraceust0 - Organ / stage
- germinating pea (Pisum sativum L.) rootse2
- Outcome
- plastids are recovered essentially free from mitochondrial and endoplasmic reticulum contaminatione1
- Value
- greater than 50%e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“greater than 50% of the plastids are recovered essentially free from mitochondrial and endoplasmic reticulum contamination”
e2“germinating pea (Pisum sativum L.) roots”
t0“pea root plastids”
Claim · model-written summary
Fatty acids synthesized from acetate by purified plastids were primarily 16:0, 16:1 and 18:1.
- Taxon
- peaLathyrus oleraceust0
- Outcome
- Fatty acids synthesized from ({sup 14}C)acetatee3
- Qualifier
- primarily 16:0, 16:1 and 18:1e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Fatty acids synthesized from ({sup 14}C)acetate by Percoll-purified plastids are primarily 16:0, 16:1 and 18:1”
t0“pea root plastids”
Claim · model-written summary
About 12% of the total glycerolipid biosynthesis activity from glycerol-3-phosphate was recovered in the purified plastid fraction.
- Taxon
- peaLathyrus oleraceust0
- Outcome
- total activity for glycerolipid biosynthesis from glycerol-3-phosphatee4
- Value
- Approximately 12%e4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Approximately 12% of the total activity for glycerolipid biosynthesis from glycerol-3-phosphate is recovered in the purified plastid fraction”
t0“pea root plastids”
Claim · model-written summary
Glycerolipids synthesized from these precursors were primarily TAG, DAG, PE, PG, PC, PI and PA.
- Taxon
- peaLathyrus oleraceust0
- Outcome
- Glycerolipids synthesized from these precursorse5
- Qualifier
- primarily TAG, DAG, PE, PG, PC, PI and PAe5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Glycerolipids synthesized from these precursors are primarily TAG, DAG, PE, PG, PC, PI and PA”
t0“pea root plastids”
Claim · model-written summary
Acetate-labelled fatty acids and glycerol-3-phosphate were both readily incorporated into glycerolipid.
- Taxon
- peaLathyrus oleraceust0
- Outcome
- readily incorporated into glycerolipide6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“({sup 14}C)Acetate-labelled fatty acids and ({sup 14}C)glycerol-3-phosphate are both readily incorporated into glycerolipid”
t0“pea root plastids”
Claim · model-written summary
Acyl-CoA accumulated when acetate was the precursor.
- Taxon
- peaLathyrus oleraceust0
- Outcome
- Acyl-CoA's also accumulatee7
- Qualifier
- when acetate is the precursore7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“Acyl-CoA's also accumulate when acetate is the precursor”
t0“pea root plastids”