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“knobs”

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Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

The knob probe signal co-localized exactly with morphologically identified knobs on maize meiotic chromosomes.

Taxon: maizeZea mayst0
Outcome: co-localization of the knob probe and the morphologically identified knobse0
Comparator: morphologically identified knobse0

Verbatim

e0
“A composite, in register, merged image of the knob probe signal and meiotic chromosomes demonstrated exact co-localization of the knob probe and the morphologically identified knobs.”
✓ located verbatim in the source · abstract, characters 643–825

t0
“maize meiotic chromosomes”
✓ located verbatim in the source · title, characters 37–62

Title

Fluorescent in situ hybridization of maize meiotic chromosomes as visualized by confocal microscopy.

E R Makowski; S E Ruzin PubMed 1994

Other claims from this paper (1)

Method / resource Confocal imaging allowed individual analysis of probe emissions, image processing, and high-resolution chromosome images.

Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

Deconvolution revealed fibrous substructure and interconnecting internal structure in perinucleolar knobs.

Taxon: Pisum sativumLathyrus oleraceuse1
Outcome: fibrous substructure in the perinucleolar knobs, and a large amount of interconnecting internal structuree0

Not reportedComparator

Verbatim

e0
“Deconvolution using the measured PSFs can be used to improve these images, revealing a fibrous substructure in the perinucleolar knobs, and a large amount of interconnecting internal structure”
✓ located verbatim in the source · abstract, characters 807–999

e1
“Pisum sativum”
✓ located verbatim in the source · abstract, characters 366–379

Title

Confocal microscopy and image processing in the study of plant nuclear structure

Peter Shaw; Martin Highett; David Rawlins Journal of Microscopy 1992. doi:10.1111/j.1365-2818.1992.tb01508.x

Other claims from this paper (5)

Observation Nearly all telomeres in both species lay at the nuclear envelope.
Observation Telomeres were highly clustered in Vicia root tissues.
Observation Pea rDNA appeared as four bright knobs around each nucleolus.
Method / resource Conventional microscopy plus deconvolution produced 3-D reconstructions comparable with confocal microscopy.
Method / resource Deconvolution of confocal data produced the clearest images.

Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

Chromosomes were identified by lengths, arm ratios, NOR knobs, and FISH signals.

Taxon: Arabidopsis thalianaArabidopsis thalianat0
Outcome: Individual chromosomes were unambiguously identifiede0
Comparator: relative lengths, arm-ratios, presence of NOR knobs and FISH signalse0

Verbatim

e0
“Individual chromosomes were unambiguously identified by their combinations of relative lengths, arm-ratios, presence of NOR knobs and FISH signals with a 5S rDNA probe and chromosome specific DNA probes.”
✓ located verbatim in the source · abstract, characters 677–880

t0
“Arabidopsis thaliana”
✓ located verbatim in the source · title, characters 34–54

Title

Cytogenetics for the model system Arabidopsis thaliana

Paul Fransz; Sue Armstrong; Carlos Alonso‐blanco; et al. The Plant Journal 1998. doi:10.1046/j.1365-313x.1998.00086.x

Other claims from this paper (12)

Method / resource A detailed Arabidopsis karyotype was presented.
Observation Five pachytene bivalents measured 50 to 80 microns.
Observation Chromosomes 1 and 5 are metacentric.
Observation Chromosomes 2 and 4 are acrocentric with subterminal NORs.
Observation Chromosome 3 is submetacentric and medium sized.
Observation Length variation between pachytene bivalents came from their short arms.
Observation Six ecotypes varied in number and map positions of 5S rDNA loci.
Observation All ecotypes have 5S rDNA on short arms of chromosomes 4 and 5.
Observation Three patterns of chromosome 3 5S rDNA locus position or presence were observed.
Method / resource Repetitive DNA clones resolved pericentromeric heterochromatin into a central and two flanking domains.
Observation Upper flanking domains of chromosomes 4 and 5, and sometimes 3, contain a 5S rDNA locus.
Method / resource Unique cosmid and YAC detection showed detailed cytogenetic physical mapping is feasible.

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