Claim · model-written summary
Dehydrating apices after 0.4 M sucrose and 2 M glycerol reduced cryogenic procedure time compared with the conventional protocol by removing the 0.8 M sucrose pre-treatment.
Verbatim
t0“Apex cryopreservation of several strawberry genotypes”
e3“Apices were encapsulated in an alginate gel, precultured on media containing high levels of sucrose (0.8 M, conventional protocol), or a combination of 0.4 M sucrose and 2 M glycerol”
e4“The latter method reduced considerably the time needed for the cryogenic procedure by eliminating the pre-treatment with 0.8 M sucrose for 19 h prior to dehydration, as required by the conventional procedure”
Title
Apex cryopreservation of several strawberry genotypes by two encapsulation-dehydration methods.
I Clavero-Ramírez; J Gálvez-Farfán; J M López-Aranda; et al. PubMed 2005