Dehydrating apices after 0.4 M sucrose and 2 M glycerol reduced cryogenicproceduretime compared with the conventional protocol by removing the 0.8 M sucrose pre-treatment.
“Apex cryopreservation of several strawberry genotypes”
e3
“Apices were encapsulated in an alginate gel, precultured on media containing high levels of sucrose (0.8 M, conventional protocol), or a combination of 0.4 M sucrose and 2 M glycerol”
e4
“The latter method reduced considerably thetimeneededforthecryogenicprocedure by eliminating the pre-treatment with 0.8 M sucrose for 19 h prior to dehydration, as required by the conventional procedure”
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Fragaria