Claim · model-written summary
The transformants showed a small decrease in phosphoribulokinase activity and larger but subproportional decreases in sedoheptulose-1,7-biphosphatase and plastid fructose-1,6-bisphosphatase activity.
- Taxon
- potato
Solanum tuberosume0 - Treatment
- Antisense expression of a full length cDNA encoding plastid aldolasee0
- Gene
- sedoheptulose-1,7-biphosphatase and plastid fructose-1,6-bisphosphatasee4
- Outcome
- phosphoribulokinase activitye4
- Direction
- decreasee4
Not reportedComparator
Verbatim
e0“Antisense expression of a full length cDNA encoding plastid aldolase led to decreased expression of aldolase at the transcript and protein level in several 'antisense' potato transformants”
e4“a small decrease of phosphoribulokinase activity, and larger but subproportional decreases of sedoheptulose-1,7-biphosphatase and plastid fructose-1,6-bisphosphatase activity”
Title
Volker Haake; Rita Zrenner; Uwe Sonnewald; et al. The Plant Journal 1998. doi:10.1046/j.1365-313x.1998.00089.x
Other claims from this paper (19)
- Intervention effect Antisense expression of plastid aldolase decreased aldolase expression at the transcript and protein level in several potato transformants.
- Intervention effect Aldolase activity was decreased by 32-43% in transformant A-70.
- Intervention effect Aldolase activity was decreased by 56-71% in transformant A-3.
- Intervention effect Aldolase activity was decreased by 79-83% in transformant A-51.
- Intervention effect Aldolase activity was decreased by 91-97% in transformant A-2.
- Mechanism Q-Sepharose separation showed that the decrease in aldolase activity was due to inhibition of plastid aldolase.
- Intervention effect The transformants showed a small increase in Rubisco activity.
- Intervention effect The transformants contained increased triose phosphates.
- Intervention effect The transformants contained very low ribulose-1,5-bisphosphate and glycerate-3-phosphate.
- Intervention effect Thylakoid energization was increased in the transformants, with photosystem II more reduced.
- Intervention effect Starch synthesis was decreased by 16% in transformant A-70.
- Intervention effect Starch synthesis was decreased by 36% in transformant A-3.
- Intervention effect Sucrose synthesis was less strongly inhibited than starch synthesis in the transformants.
- Intervention effect Ambient photosynthesis was inhibited by 10% in transformant A-70.
- Intervention effect Ambient photosynthesis was inhibited by 40% in transformant A-3.
- Intervention effect Ambient photosynthesis was inhibited by 66% in transformant A-51.
- Intervention effect Ambient photosynthesis was inhibited by 85% in transformant A-2.
- Intervention effect Plant growth was not significantly altered in transformant A-70.
- Intervention effect Plant growth was decreased by 41% in transformant A-3 and was severely inhibited in plants with under 20% of wild-type aldolase activity.