Claim · model-written summary
Fifteen RGA clones were isolated from cDNA of rust race I infected leaves sampled 24 hours after infection.
Coffea arabicaVerbatim
t0“(Coffea Arabica L. Cv S.288)”
e4“Fifteen RGA clones isolated from cDNA prepared from rust race I infected leaf sample for 24 hours”
Title
Deepak Kumar Journal of Biotechnology & Biomaterials 2012. doi:10.4172/2155-952x.1000146
Other claims from this paper (10)
- Observation Seven RGA were isolated from S.288 genomic DNA using non-degenerate primers.
- Observation Eleven more RGA were isolated from S.288 genomic DNA using degenerate primers.
- Observation Thirty-two RGA were isolated from cDNA of S.288.
- Observation C.arabica RGA showed a high level of similarity with C.canephora RGA by BLASTN.
- Observation RGA of length below 475 bp or above 530 bp had either no match with any RGA or matched microsatellites.
- Observation One independent sequence from genomic DNA gave no BLASTN result.
- Observation Four independent sequences from cDNA gave no BLASTN result.
- Observation Some of the unmatched sequences may belong to a new type of RGA.
- Observation The RGA reported in this study are mainly of the class A type.
- Mechanism A difference in expression profile might be due to sequence-level differences between RGA isolated from DNA and cDNA.