Claim · model-written summary
Carbon T1 relaxation times declined by an order of magnitude on hydration.
- Taxon
- wheat
Triticum aestivumt0 - Outcome
- Carbon T1 relaxation timese0
- Direction
- decreasee0
- Value
- an order of magnitudee0
Verbatim
e0“Carbon T1 relaxation times reflect the general mobility increase, in the MHz frequency range, by showing an order of magnitude decrease upon hydration.”
t0“wheat”
Title
Magic angle spinning NMR study of the hydration of the wheat seed storage protein omega-gliadins
A. M. Gil; E. Alberti; A. S. Tatham; et al. Magnetic Resonance in Chemistry 1997. doi:10.1002/(sici)1097-458x(199712)35:13<s101::aid-omr191>3.0.co;2-u
Other claims from this paper (11)
- Observation Increasing hydration from 0 to 50% increased general omega-gliadin mobility.
- Observation Some glutamine side-chain carbons remained relatively immobilized at 50% hydration.
- Observation Results suggest a looser protein conformation at about 35% hydration.
- Observation Backbone and glutamine side-chain carbonyl T1 values were indistinguishable.
- Method / resource A new high-resolution MAS probe improved hydrated omega-gliadin proton spectrum resolution.
- Observation Some phenylalanine residues were substantially motionally hindered.
- Observation Some glutamine side-chain amino groups were inaccessible to solvent.
- Mechanism The authors propose hydrophobic pockets held by hydrogen bonding between glutamine residues in hydrated omega-gliadins.
- Method / resource The new probe permitted two-dimensional experiments for assignments and conformational investigation.
- Association Under MAS, proton T1 relaxation times depended strongly on spinning rate.
- Recommendation The authors caution about interpreting proton relaxation times measured under MAS.