Claim · model-written summary
After salt treatment, 2556 differentially expressed genes were specifically upregulated and 45 downregulated.
Triticum aestivumVerbatim
e0“the expression of 2556 DEGs was specifically up-regulated after salt treatment, while the expression of 45 DEGs was specifically down-regulated”
t0“wheat”
Title
The bZIP transcription factor TabZIP15 improves salt stress tolerance in wheat
Chenxi Bi; Yuehua Yu; Chunhao Dong; et al. Plant Biotechnology Journal 2020. doi:10.1111/pbi.13453
Other claims from this paper (19)
- Observation TabZIP15 was identified as salt responsive in wheat.
- Observation TabZIP15 has six exons and five introns and encodes a bZIP transcription factor.
- Intervention effect Seedling root TabZIP15 expression peaked within an hour of salt treatment at about 2.4 times pretreatment expression.
- Observation TabZIP15 is nuclear localized and activates transcription in yeast.
- Observation TabZIP15 binds the ABA-responsive element in a yeast assay.
- Intervention effect Under normal conditions, transgenic and KN199 wheat had no obvious phenotypic difference.
- Intervention effect After 20 days of 300 mm NaCl, TabZIP15 transgenic wheat wilted less than KN199.
- Intervention effect Salt treated transgenic wheat had greater height and aboveground fresh weight.
- Intervention effect Salt treated transgenic wheat had lower malondialdehyde and H2O2 contents.
- Intervention effect Under normal conditions, root morphology, length and fresh weight did not obviously differ between transgenic and KN199 wheat.
- Intervention effect Under salt stress, transgenic wheat had a larger root system and greater root length and fresh weight than KN199.
- Intervention effect Transgenic and KN199 wheat spikes were almost the same under normal growth.
- Intervention effect Transgenic wheat had longer spikes and significantly more grains per spike than KN199.
- Observation TaENO-b physically interacted with TabZIP15, including confirmation in Nicotiana benthamiana.
- Observation At zero hours, H381 and KN199 differed in 3332 genes, with 1297 upregulated and 2035 downregulated in H381.
- Observation At one hour, H381 and KN199 differed in 2807 genes, with 2691 upregulated and 116 downregulated in H381.
- Observation The 2556 upregulated genes were enriched in 20 biological processes, including metabolism, iron ion binding and abiotic response.
- Observation More than 900 genes were enriched in metabolic processes and more than 30 in abiotic stimulus response.
- Recommendation The authors propose that manipulating TabZIP15 expression enhances wheat salt tolerance.