Claim · model-written summary
The authors propose that manipulating TabZIP15 expression enhances wheat salt tolerance.
- Taxon
- wheat
Triticum aestivumt0 - Outcome
- manipulating the expression of this gene leads to enhanced salt stress tolerancee0
Verbatim
e0“we propose that manipulating the expression of this gene leads to enhanced salt stress tolerance”
t0“wheat”
Title
The bZIP transcription factor TabZIP15 improves salt stress tolerance in wheat
Chenxi Bi; Yuehua Yu; Chunhao Dong; et al. Plant Biotechnology Journal 2020. doi:10.1111/pbi.13453
Other claims from this paper (19)
- Observation TabZIP15 was identified as salt responsive in wheat.
- Observation TabZIP15 has six exons and five introns and encodes a bZIP transcription factor.
- Intervention effect Seedling root TabZIP15 expression peaked within an hour of salt treatment at about 2.4 times pretreatment expression.
- Observation TabZIP15 is nuclear localized and activates transcription in yeast.
- Observation TabZIP15 binds the ABA-responsive element in a yeast assay.
- Intervention effect Under normal conditions, transgenic and KN199 wheat had no obvious phenotypic difference.
- Intervention effect After 20 days of 300 mm NaCl, TabZIP15 transgenic wheat wilted less than KN199.
- Intervention effect Salt treated transgenic wheat had greater height and aboveground fresh weight.
- Intervention effect Salt treated transgenic wheat had lower malondialdehyde and H2O2 contents.
- Intervention effect Under normal conditions, root morphology, length and fresh weight did not obviously differ between transgenic and KN199 wheat.
- Intervention effect Under salt stress, transgenic wheat had a larger root system and greater root length and fresh weight than KN199.
- Intervention effect Transgenic and KN199 wheat spikes were almost the same under normal growth.
- Intervention effect Transgenic wheat had longer spikes and significantly more grains per spike than KN199.
- Observation TaENO-b physically interacted with TabZIP15, including confirmation in Nicotiana benthamiana.
- Observation At zero hours, H381 and KN199 differed in 3332 genes, with 1297 upregulated and 2035 downregulated in H381.
- Observation At one hour, H381 and KN199 differed in 2807 genes, with 2691 upregulated and 116 downregulated in H381.
- Observation After salt treatment, 2556 differentially expressed genes were specifically upregulated and 45 downregulated.
- Observation The 2556 upregulated genes were enriched in 20 biological processes, including metabolism, iron ion binding and abiotic response.
- Observation More than 900 genes were enriched in metabolic processes and more than 30 in abiotic stimulus response.