Claim · model-written summary
The early stage of somatic embryogenesis in Cyathea delgadii proceeds by sequential perpendicular cell divisions of a single epidermal cell of an etiolated stipe explant.
- Taxon
- Cyathea delgadii
Cyathea delgadiie1 - Organ / stage
- etiolated stipe explante3
- Outcome
- sequential perpendicular cell divisionse3
- Qualifier
- of an individual epidermal celle3
Verbatim
e1“We discovered that the tree fern Cyathea delgadii offers an effective model for the reproducible and rapid formation of somatic embryos on hormone-free medium”
e3“The early stage of SE was characterized by sequential perpendicular cell divisions of an individual epidermal cell of etiolated stipe explant”
Title
Somatic embryogenesis in ferns: a new experimental system
Anna Mikuła; Mariusz Pożoga; Karolina Tomiczak; et al. Plant Cell Reports 2015. doi:10.1007/s00299-015-1741-9
Other claims from this paper (7)
- Observation Cyathea delgadii formed somatic embryos reproducibly and rapidly on hormone-free medium.
- Observation Somatic embryogenesis was induced in primary and secondary explants of Cyathea delgadii without exogenous plant growth regulators, in darkness.
- Observation Three morphogenetic stages of fern somatic embryo development were defined: linear, early and late embryonic leaf stage.
- Observation The transition from somatic embryo to juvenile sporophyte in Cyathea delgadii was quick.
- Observation After 9 weeks of culture, the efficiency of somatic embryogenesis in Cyathea delgadii reached 12-13 embryos per responding explant.
- Observation Spontaneous somatic embryos and callus production improved the effectiveness of somatic embryogenesis sevenfold in a 10-month culture.
- Observation Embryogenic competence is much easier to evoke in ferns than in spermatophytes.