By interacting organism › Other organisms › Cryptosporidiidae

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

The mNGS assay identified as few as 100 oocysts of C. parvum in 25 g of fresh lettuce.

Taxon: fresh lettucee2
Interactor: C. parvumCryptosporidium parvume2other
Outcome: identificatione2
Value: 100 oocystse2

Verbatim

e1
“Lettuce (25 g) was spiked with varying numbers of Cryptosporidium parvum oocysts”
✓ located verbatim in the source · abstract, characters 575–655

e2
“Nanopore sequencing followed by bioinformatic analysis led to the consistent identification of as few as 100 oocysts of C. parvum in 25 g of fresh lettuce”
✓ located verbatim in the source · abstract, characters 1588–1742

Title

Metagenomic detection of protozoan parasites on leafy greens aided by a rapid and efficient DNA extraction protocol

Sohail Naushad; Ruimin Gao; Marc-Olivier Duceppe; et al. Frontiers in Microbiology 2025. doi:10.3389/fmicb.2025.1566579

Other claims from this paper (4)

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

The assay simultaneously detected C. parvum and G. duodenalis on lettuce, among other protozoa.

Taxon: LettuceLactuca sativae1
Interactor: C. parvumCryptosporidium parvume4other
Second interactor: G. duodenalisGiardia duodenalise4other
Outcome: simultaneous detectione4
Qualifier: C. hominis, C. muris, G. duodenalis and T. gondiie4

Verbatim

e1
“Lettuce (25 g) was spiked with varying numbers of Cryptosporidium parvum oocysts”
✓ located verbatim in the source · abstract, characters 575–655

e4
“The assay proved useful for the simultaneous detection of C. parvum, C. hominis, C. muris, G. duodenalis and T. gondii”
✓ located verbatim in the source · abstract, characters 1812–1930

Title

Metagenomic detection of protozoan parasites on leafy greens aided by a rapid and efficient DNA extraction protocol

Sohail Naushad; Ruimin Gao; Marc-Olivier Duceppe; et al. Frontiers in Microbiology 2025. doi:10.3389/fmicb.2025.1566579

Other claims from this paper (4)

Method / resource The mNGS assay identified as few as 100 oocysts of C. parvum in 25 g of fresh lettuce.
Method / resource Ion S5 sequencing gave results similar to Nanopore sequencing for the mNGS assay on lettuce.
Method / resource Amplification gave 0.16–8.25 μg of DNA from lettuce washes, sufficient to perform mNGS.
Method / resource Lysis of oocysts and cysts was rapidly achieved within 3 min on lettuce, a prerequisite for sensitive detection.

Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Cryptosporidium parvum oocysts were the most common parasite, occurring in 76.25% of samples.

Taxon: vegetablest0
Interactor: Cryptosporidium parvumCryptosporidium parvume0pathogen
Outcome: Cryptosporidium parvum oocystse0
Value: 76.25% (183/240)e0

Verbatim

e0
“The most encountered contaminating parasitewas Cryptosporidium parvum oocysts 76.25% (183/240)”
✓ located verbatim in the source · abstract, characters 899–993

t0
“VEGETABLES”
✓ located verbatim in the source · title, characters 52–62

Title

DETECTION OF PARASITES CONTAMINATING RAW CONSUMABLE VEGETABLES IN ASSIUT CITY, ASSIUT GOVERNORATE, EGYPT

AYA A. AHMED; FATMA G. SAYED; LAMIA A. GALAL; et al. Journal of the Egyptian Society of Parasitology 2020. doi:10.21608/jesp.2020.131086

Other claims from this paper (7)

Observation Parasites contaminated 84.1% of sampled vegetables.
Observation Parsley was the most contaminated vegetable.
Observation Lettuce was the least contaminated vegetable.
Observation Cryptosporidium parvum was found in 38 of 40 radish samples.
Observation Isospora sp. was least detected, at 2.1%.
Observation Parasites were viable in half of tested samples.
Recommendation The authors urge alternative farming, irrigation, and washing methods locally.

Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Cryptosporidium parvum was found in 38 of 40 radish samples.

Taxon: radishRaphanus sativuse0
Interactor: Cryptosporidium parvumCryptosporidium parvume1pathogen
Outcome: most frequente0
Value: 38/40e0

Verbatim

e0
“most frequent in radish (38/40)”
✓ located verbatim in the source · abstract, characters 994–1025

e1
“Cryptosporidium parvum oocysts 76.25% (183/240) most frequent in radish (38/40)”
✓ located verbatim in the source · abstract, characters 946–1025

t0
“VEGETABLES”
✓ located verbatim in the source · title, characters 52–62

Title

DETECTION OF PARASITES CONTAMINATING RAW CONSUMABLE VEGETABLES IN ASSIUT CITY, ASSIUT GOVERNORATE, EGYPT

AYA A. AHMED; FATMA G. SAYED; LAMIA A. GALAL; et al. Journal of the Egyptian Society of Parasitology 2020. doi:10.21608/jesp.2020.131086

Other claims from this paper (7)

Observation Parasites contaminated 84.1% of sampled vegetables.
Observation Parsley was the most contaminated vegetable.
Observation Lettuce was the least contaminated vegetable.
Observation Cryptosporidium parvum oocysts were the most common parasite, occurring in 76.25% of samples.
Observation Isospora sp. was least detected, at 2.1%.
Observation Parasites were viable in half of tested samples.
Recommendation The authors urge alternative farming, irrigation, and washing methods locally.

Observationobservedoriginal resultpositivesimulated

Claim · model-written summary

Estimated annual infection risk from Cryptosporidium through lettuce is 10−6–10−8.

Taxon: lettuceLactuca sativae2
Interactor: CryptosporidiumCryptosporidiume4pathogen
Outcome: estimated annual infection riske4
Value: 10−6–10−8e4

Verbatim

e4
“The estimated annual infection risk for Cryptosporidium, Campylobacter, and Norovirus was 10−6–10−8, 10−8–10−10, and 10−10–10−11 respectively”
✓ located verbatim in the source · abstract, characters 1114–1255

e2
“The final effluent from the system was used to grow three lettuce varieties by adding urine as a nutrient solution”
✓ located verbatim in the source · abstract, characters 741–855

Title

Treated Greywater Reuse for Hydroponic Lettuce Production in a Green Wall System: Quantitative Health Risk Assessment

Fasil Eregno; Melesse Moges; Arve Heistad Water 2017. doi:10.3390/w9070454

Other claims from this paper (6)

Cryptosporidiidae 5 claims

5 claims