Claim · model-written summary
Tomato plants inoculated with C. murale sap showed the same leaf symptoms as originally observed.
- Taxon
- tomato
Solanum lycopersicume0 - Treatment
- inoculated with C. murale plant sape0
- Outcome
- same leaf symptoms as those originally observede0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“infecting tomato”
e0“The tomato plants subsequently inoculated with C. murale plant sap showed the same leaf symptoms as those originally observed”
Title
First report of Tomato brown rugose fruit virus infecting tomato in Germany
W. Menzel; D. Knierim; S. Winter; et al. New Disease Reports 2019. doi:10.5197/j.2044-0588.2019.039.001
Other claims from this paper (10)
- Observation This is the first report of Tomato brown rugose fruit virus in Europe and outside countries of the Near East.
- Observation Unusual fruit and leaf symptoms were observed in numerous commercial tomato greenhouses in the Lower Rhine region of North-Rhine Westphalia, Germany.
- Observation More than 25 ha of greenhouse tomatoes were affected.
- Observation Electron microscopy showed filamentous virus-like particles later identified as Pepino mosaic virus Chile 2 strain and particles with a tobamovirus-like structure.
- Observation RT-PCR with universal tobamovirus primers gave amplicons of the expected size of about 560 bp for all symptomatic samples.
- Observation Blastn analysis of the sequenced PCR products revealed 100% nucleotide identity to Tomato brown rugose fruit virus.
- Observation Genome sequences of two German ToBRFV isolates showed 99.6% nucleotide sequence identity to each other.
- Observation Sequence identities of the German isolates to isolates from Jordan and Israel were 99.7-99.8%, pointing to a common origin.
- Observation ToBRFV was isolated from a mixed infection with PepMV by passage on Chenopodium murale.
- Method / resource The ToBRFV isolate P12-3G was deposited as PV-1236 in the DSMZ Plant Virus Collection.