Claim · model-written summary
Sequence identities of the German isolates to isolates from Jordan and Israel were 99.7-99.8%, pointing to a common origin.
- Taxon
- tomato
Solanum lycopersicumt0 - Outcome
- sequence identitiese0
- Value
- 99.7-99.8%e0
- Comparator
- isolates from Jordan and Israele0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentSettingDirectionQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“infecting tomato”
e0“The sequence identities to the isolates from Jordan and Israel were in the same range (99.7-99.8%)”
Title
First report of Tomato brown rugose fruit virus infecting tomato in Germany
W. Menzel; D. Knierim; S. Winter; et al. New Disease Reports 2019. doi:10.5197/j.2044-0588.2019.039.001
Other claims from this paper (10)
- Observation This is the first report of Tomato brown rugose fruit virus in Europe and outside countries of the Near East.
- Observation Unusual fruit and leaf symptoms were observed in numerous commercial tomato greenhouses in the Lower Rhine region of North-Rhine Westphalia, Germany.
- Observation More than 25 ha of greenhouse tomatoes were affected.
- Observation Electron microscopy showed filamentous virus-like particles later identified as Pepino mosaic virus Chile 2 strain and particles with a tobamovirus-like structure.
- Observation RT-PCR with universal tobamovirus primers gave amplicons of the expected size of about 560 bp for all symptomatic samples.
- Observation Blastn analysis of the sequenced PCR products revealed 100% nucleotide identity to Tomato brown rugose fruit virus.
- Observation Genome sequences of two German ToBRFV isolates showed 99.6% nucleotide sequence identity to each other.
- Observation ToBRFV was isolated from a mixed infection with PepMV by passage on Chenopodium murale.
- Observation Tomato plants inoculated with C. murale sap showed the same leaf symptoms as originally observed.
- Method / resource The ToBRFV isolate P12-3G was deposited as PV-1236 in the DSMZ Plant Virus Collection.