Claim · model-written summary
Mitochondrial swelling was completely prevented in necrotic cells compared with control cells.
- Taxon
- soybean
Glycine maxt0 - Treatment
- necrotice0
- Outcome
- swellinge0
- Direction
- completely preventede0
- Comparator
- control cellse0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleSettingValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mitochondria isolated from control cells still underwent swelling, which was partially or completely prevented in mitochondria isolated from apoptotic or necrotic cells, respectively.”
t0“soybean suspension cell cultures”
Title
V. Casolo Journal of Experimental Botany 2005. doi:10.1093/jxb/eri093
Other claims from this paper (16)
- Intervention effect Hydrogen peroxide at 5 mM induced programmed cell death.
- Intervention effect Cells treated with 20 mM H2O2 became necrotic, with intermediate necrapoptotic hallmarks before that.
- Observation ATP and glucose-6-phosphate stayed constant during programmed cell death.
- Observation ATP and glucose-6-phosphate decreased significantly in necrotic cells.
- Intervention effect Succinate-dependent oxygen consumption was slightly uncoupled in mitochondria from 5 mM H2O2-treated cells.
- Intervention effect Mitochondrial electrical potential difference weakly decreased after 5 mM H2O2 treatment.
- Intervention effect KCl caused greater partial dissipation of delta psi in treated mitochondria than in controls.
- Mechanism CsA induced delta psi formation in de-energized mitochondria through K+ efflux.
- Intervention effect CsA-induced K+ efflux was reduced in mitochondria from treated cells.
- Observation Mitochondria from 1 mM sodium nitroprusside-treated cells showed the same response pattern and apoptotic symptoms.
- Intervention effect Succinate-dependent oxygen consumption was completely uncoupled in necrotic cells treated with 20 mM H2O2.
- Intervention effect Delta psi generation was significantly inhibited in necrotic cells treated with 20 mM H2O2.
- Intervention effect CsA-dependent delta psi formation was prevented in necrotic cells treated with 20 mM H2O2.
- Intervention effect Mitochondrial swelling was partly prevented in apoptotic cells compared with control cells.
- Observation Moderate swelling accompanied slight outer-membrane rupture and cytochrome c release.
- Mechanism The authors implicate a K(+)ATP channel in H2O2- or NO-induced programmed cell death.