Abstract
Soybean suspension cell cultures were treated by H2O2 or nitric oxide (NO), to assess the mechanism leading to programmed cell death (PCD). Hydrogen peroxide (5 mM) induced PCD. Cells become necrotic at 20 mM H2O2, with cells exhibiting intermediate hallmarks before that (necrapoptotic cells). The level of ATP and of glucose-6-phosphate remained constant in cells undergoing PCD, while it decreased significantly in the necrotic ones. Mitochondria, isolated from 5 mM H2O2-treated (apoptotic) cells, showed that succinate-dependent oxygen consumption was slightly uncoupled, and the electrical potential difference (delta psi) weakly decreased. The addition of KCl to the delta psi formed determined a partial dissipation, which was higher than the dissipation observed in mitochondria from control cells. The addition of cyclosporin A (CsA) to de-energized mitochondria also induced delta psi formation, due to a K+ efflux from the matrix, which was decreased in mitochondria from treated cells. The same pattern of response was also observed in mitochondria isolated from 1 mM sodium nitroprusside (NO)-treated cells, exhibiting apoptotic symptoms. In mitochondria isolated from 20 mM H2O2-treated (necrotic) cells, succinate-dependent oxygen consumption was completely uncoupled, delta psi generation significantly inhibited, and CsA-dependent delta psi formation prevented. In addition, mitochondria isolated from control cells still underwent swelling, which was partially or completely prevented in mitochondria isolated from apoptotic or necrotic cells, respectively. The moderate swelling was accompanied by a slight rupture of the outer membrane and by a release of cytochrome c. These results point to the involvement of a K(+)ATP channel during the manifestation of PCD induced by H2O2 or NO in plants.
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Claims 17
Claim · model-written summary
Hydrogen peroxide at 5 mM induced programmed cell death.
- Taxon
- soybean
Glycine maxt0 - Treatment
- Hydrogen peroxide (5 mM)e0
- Outcome
- PCDe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hydrogen peroxide (5 mM) induced PCD.”
t0“soybean suspension cell cultures”
Claim · model-written summary
Cells treated with 20 mM H2O2 became necrotic, with intermediate necrapoptotic hallmarks before that.
- Taxon
- soybeanGlycine maxt0
- Treatment
- 20 mM H2O2e0
- Outcome
- necrotice0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cells become necrotic at 20 mM H2O2, with cells exhibiting intermediate hallmarks before that (necrapoptotic cells).”
t0“soybean suspension cell cultures”
Claim · model-written summary
ATP and glucose-6-phosphate stayed constant during programmed cell death.
- Taxon
- soybeanGlycine maxt0
- Outcome
- The level of ATP and of glucose-6-phosphatee0
- Direction
- remained constante0
- Qualifier
- cells undergoing PCDe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The level of ATP and of glucose-6-phosphate remained constant in cells undergoing PCD”
t0“soybean suspension cell cultures”
Claim · model-written summary
ATP and glucose-6-phosphate decreased significantly in necrotic cells.
- Taxon
- soybeanGlycine maxt0
- Outcome
- The level of ATP and of glucose-6-phosphatee0
- Direction
- decreasede0
- Qualifier
- necrotic onese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The level of ATP and of glucose-6-phosphate remained constant in cells undergoing PCD, while it decreased significantly in the necrotic ones.”
t0“soybean suspension cell cultures”
Claim · model-written summary
Succinate-dependent oxygen consumption was slightly uncoupled in mitochondria from 5 mM H2O2-treated cells.
- Taxon
- soybeanGlycine maxt0
- Treatment
- 5 mM H2O2-treatede0
- Outcome
- succinate-dependent oxygen consumptione0
- Direction
- slightly uncouplede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mitochondria, isolated from 5 mM H2O2-treated (apoptotic) cells, showed that succinate-dependent oxygen consumption was slightly uncoupled”
t0“soybean suspension cell cultures”
Claim · model-written summary
Mitochondrial electrical potential difference weakly decreased after 5 mM H2O2 treatment.
- Taxon
- soybeanGlycine maxt0
- Treatment
- 5 mM H2O2-treatede0
- Outcome
- electrical potential difference (delta psi)e0
- Direction
- weakly decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mitochondria, isolated from 5 mM H2O2-treated (apoptotic) cells, showed that succinate-dependent oxygen consumption was slightly uncoupled, and the electrical potential difference (delta psi) weakly decreased.”
t0“soybean suspension cell cultures”
Claim · model-written summary
KCl caused greater partial dissipation of delta psi in treated mitochondria than in controls.
- Taxon
- soybeanGlycine maxt0
- Treatment
- The addition of KCle0
- Outcome
- partial dissipatione0
- Direction
- highere0
- Comparator
- control cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The addition of KCl to the delta psi formed determined a partial dissipation, which was higher than the dissipation observed in mitochondria from control cells.”
t0“soybean suspension cell cultures”
Claim · model-written summary
CsA induced delta psi formation in de-energized mitochondria through K+ efflux.
- Taxon
- soybeanGlycine maxt0
- Treatment
- cyclosporin A (CsA)e0
- Outcome
- delta psi formatione0
- Qualifier
- a K+ efflux from the matrixe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The addition of cyclosporin A (CsA) to de-energized mitochondria also induced delta psi formation, due to a K+ efflux from the matrix”
t0“soybean suspension cell cultures”
Claim · model-written summary
CsA-induced K+ efflux was reduced in mitochondria from treated cells.
- Taxon
- soybeanGlycine maxt0
- Treatment
- treated cellse0
- Outcome
- K+ effluxe0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“due to a K+ efflux from the matrix, which was decreased in mitochondria from treated cells.”
t0“soybean suspension cell cultures”
Claim · model-written summary
Mitochondria from 1 mM sodium nitroprusside-treated cells showed the same response pattern and apoptotic symptoms.
- Taxon
- soybeanGlycine maxt0
- Treatment
- 1 mM sodium nitroprusside (NO)-treatede0
- Outcome
- apoptotic symptomse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The same pattern of response was also observed in mitochondria isolated from 1 mM sodium nitroprusside (NO)-treated cells, exhibiting apoptotic symptoms.”
t0“soybean suspension cell cultures”
Claim · model-written summary
Succinate-dependent oxygen consumption was completely uncoupled in necrotic cells treated with 20 mM H2O2.
- Taxon
- soybeanGlycine maxt0
- Treatment
- 20 mM H2O2-treatede0
- Outcome
- succinate-dependent oxygen consumptione0
- Direction
- completely uncouplede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In mitochondria isolated from 20 mM H2O2-treated (necrotic) cells, succinate-dependent oxygen consumption was completely uncoupled”
t0“soybean suspension cell cultures”
Claim · model-written summary
Delta psi generation was significantly inhibited in necrotic cells treated with 20 mM H2O2.
- Taxon
- soybeanGlycine maxt0
- Treatment
- 20 mM H2O2-treatede0
- Outcome
- delta psi generatione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In mitochondria isolated from 20 mM H2O2-treated (necrotic) cells, succinate-dependent oxygen consumption was completely uncoupled, delta psi generation significantly inhibited”
t0“soybean suspension cell cultures”
Claim · model-written summary
CsA-dependent delta psi formation was prevented in necrotic cells treated with 20 mM H2O2.
- Taxon
- soybeanGlycine maxt0
- Treatment
- 20 mM H2O2-treatede0
- Outcome
- CsA-dependent delta psi formatione0
- Direction
- preventede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In mitochondria isolated from 20 mM H2O2-treated (necrotic) cells, succinate-dependent oxygen consumption was completely uncoupled, delta psi generation significantly inhibited, and CsA-dependent delta psi formation prevented.”
t0“soybean suspension cell cultures”
Claim · model-written summary
Mitochondrial swelling was partly prevented in apoptotic cells compared with control cells.
- Taxon
- soybeanGlycine maxt0
- Treatment
- apoptotice0
- Outcome
- swellinge0
- Direction
- partiallye0
- Comparator
- control cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mitochondria isolated from control cells still underwent swelling, which was partially or completely prevented in mitochondria isolated from apoptotic or necrotic cells, respectively.”
t0“soybean suspension cell cultures”
Claim · model-written summary
Mitochondrial swelling was completely prevented in necrotic cells compared with control cells.
- Taxon
- soybeanGlycine maxt0
- Treatment
- necrotice0
- Outcome
- swellinge0
- Direction
- completely preventede0
- Comparator
- control cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mitochondria isolated from control cells still underwent swelling, which was partially or completely prevented in mitochondria isolated from apoptotic or necrotic cells, respectively.”
t0“soybean suspension cell cultures”
Claim · model-written summary
Moderate swelling accompanied slight outer-membrane rupture and cytochrome c release.
- Taxon
- soybeanGlycine maxt0
- Outcome
- a slight rupture of the outer membrane and by a release of cytochrome ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The moderate swelling was accompanied by a slight rupture of the outer membrane and by a release of cytochrome c.”
t0“soybean suspension cell cultures”
Claim · model-written summary
The authors implicate a K(+)ATP channel in H2O2- or NO-induced programmed cell death.
- Taxon
- soybeanGlycine maxt0
- Gene
- K(+)ATP channele0
- Outcome
- PCDe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These results point to the involvement of a K(+)ATP channel during the manifestation of PCD induced by H2O2 or NO in plants.”
t0“soybean suspension cell cultures”
The same questions in other papers
hydrogen peroxide · molecular: metabolites · Glycine max (soybean)Positive and negative results
1 paper in the same direction as this paper · 4 in the opposite direction
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →hydrogen peroxide · cytology · Glycine max (soybean)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.