Claim · model-written summary
On a medium with 2.5 µM picloram, somatic embryos were induced directly from pea meristematic tissues without a callus phase.
- Taxon
- pea
Lathyrus oleraceust0 - Organ / stage
- meristematic tissuese1
- Treatment
- medium supplemented with 2.5 µM piclorame1
- Outcome
- Somatic embryos were induced directlye1
Not reportedMaterialGeneCompoundInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“meristems of pea”
e1“Somatic embryos were induced directly (without callus intervention) from meristematic tissues grown on a medium supplemented with 2.5 µM picloram”
Title
M. Griga Biologia Plantarum 1998. doi:10.1023/a:1001834213437
Other claims from this paper (10)
- Observation Fully morphologically developed somatic embryos were obtained within 4 to 5 weeks.
- Observation Pea somatic embryos originated from both apical and basal parts of the meristem explants.
- Observation Initiation and development of pea somatic embryos was asynchronous.
- Observation Basal somatic embryos developed more quickly than apical ones.
- Observation Abundant secondary embryogenesis occurred after primary somatic embryos were isolated and cultured on media for germination.
- Intervention effect Morphologically normal somatic embryos germinated on medium without growth regulators, and the conversion rate was increased by application of 10 µM thidiazuron.
- Intervention effect Thidiazuron induced shoot bud regeneration on somatic embryos lacking a differentiated shoot apex.
- Intervention effect With thidiazuron, up to 78 % of all harvested somatic embryos of various morphology could germinate.
- Method / resource The somatic embryogenesis protocol worked in 47 out of 48 P. sativum and P. arvense cultivars.
- Method / resource The somatic embryogenesis protocol also worked in two wild peas (P. elatius and P. jomardi).