Abstract
Abstract The effect of different shoot culture compositions on the thin cell layer nodal (TCLn) regeneration system for pea was tested. By varying the composition of the shoot culture medium, up to 86% of the regenerated shoots in cultivar Terese were able to produce roots at standard rooting conditions. Addition of silver nitrate to the shoot culture medium decreased vitrification, but greatly reduced rooting frequencies There was no major influence on shoot regeneration or rooting capacity when using 1.5% or 3.0% glucose or sucrose The use of 5 × 15−6M indole butyric acid in the rooting medium did not increase rooting. Seed‐derived progeny of regenerates contained the normal diploid number of chromosomes (2n = 14). It was demonstrated that rooting of shoots arising from TCLn explants was influenced more by the composition of the regeneration medium than by the rooting medium. These results imply a rather long‐lasting effect of the components in the regeneration medium. Key words: carbohydrate sourcechromosome numberindole butyric acidmedium compositionsilver nitratetissue culturevitrification Notes Corresponding author.
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Claims 8
Claim · model-written summary
Up to 86% of regenerated shoots of cultivar Terese rooted under standard rooting conditions with changed shoot medium composition.
- Taxon
- shootse0
- Material
- cultivar Teresee0
- Treatment
- varying the composition of the shoot culture mediume0
- Outcome
- produce rootse0
- Value
- 86%e0
Not reportedComparatorDirection
Verbatim
e0“By varying the composition of the shoot culture medium, up to 86% of the regenerated shoots in cultivar Terese were able to produce roots at standard rooting conditions.”
Claim · model-written summary
Silver nitrate addition decreased vitrification.
- Taxon
- pea
Lathyrus oleraceust0 - Treatment
- Addition of silver nitrate to the shoot culture mediume0
- Outcome
- vitrificatione0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of silver nitrate to the shoot culture medium decreased vitrification”
t0“pea”
Claim · model-written summary
Silver nitrate addition greatly reduced rooting frequency.
- Taxon
- peaLathyrus oleraceust0
- Treatment
- Addition of silver nitrate to the shoot culture mediume0
- Outcome
- rooting frequenciese0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of silver nitrate to the shoot culture medium decreased vitrification, but greatly reduced rooting frequencies”
t0“pea”
Claim · model-written summary
Changing glucose concentration between 1.5% and 3.0% had no major influence on shoot regeneration or rooting capacity.
- Taxon
- peaLathyrus oleraceust0
- Treatment
- 1.5% or 3.0% glucosee0
- Outcome
- shoot regeneration or rooting capacitye0
- Direction
- no major influencee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There was no major influence on shoot regeneration or rooting capacity when using 1.5% or 3.0% glucose or sucrose”
t0“pea”
Claim · model-written summary
Changing sucrose concentration between 1.5% and 3.0% had no major influence on shoot regeneration or rooting capacity.
- Taxon
- peaLathyrus oleraceust0
- Treatment
- 1.5% or 3.0% glucose or sucrosee0
- Outcome
- shoot regeneration or rooting capacitye0
- Direction
- no major influencee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There was no major influence on shoot regeneration or rooting capacity when using 1.5% or 3.0% glucose or sucrose”
t0“pea”
Claim · model-written summary
Indole butyric acid in rooting medium did not increase rooting.
- Taxon
- peaLathyrus oleraceust0
- Treatment
- 5 × 15−6M indole butyric acide0
- Outcome
- rootinge0
- Direction
- did not increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The use of 5 × 15−6M indole butyric acid in the rooting medium did not increase rooting.”
t0“pea”
Claim · model-written summary
Seed-derived progeny of regenerates had the normal diploid chromosome number of 2n = 14.
- Taxon
- peaLathyrus oleraceust0
- Outcome
- normal diploid number of chromosomese0
- Value
- 2n = 14e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Seed‐derived progeny of regenerates contained the normal diploid number of chromosomes (2n = 14).”
t0“pea”
Claim · model-written summary
Regeneration medium composition influenced rooting more than rooting medium composition.
- Taxon
- peaLathyrus oleraceust0
- Treatment
- composition of the regeneration mediume0
- Outcome
- rooting of shoots arising from TCLn explantse0
- Direction
- moree0
- Comparator
- rooting mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rooting of shoots arising from TCLn explants was influenced more by the composition of the regeneration medium than by the rooting medium”
t0“pea”
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