Claim · model-written summary
EDTA and NEM pretreatments affected bound ATP labeling and phosphorylation similarly.
- Taxon
- spinach
Spinacia oleraceae1 - Treatment
- EDTA and NEM pretreatmentse0
- Outcome
- both reactionse0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“EDTA and NEM pretreatments of the chloroplasts affect both reactions similarly”
e1“spinach chloroplasts”
Title
Possible role of firmly bound ATP in the energy transduction of photosynthetic membranes
Hans U. Lutz; Walter Beyeler; Christian Pflugshaupt; et al. Journal of Supramolecular Structure 1975. doi:10.1002/jss.400030509
Other claims from this paper (13)
- Observation Spinach chloroplasts contain firmly bound ATP rapidly labeled with ADP in the presence of 32Pi and endogenous nucleotides.
- Observation Labeling of bound ATP was not entirely dependent on light.
- Observation Three ATP pools in chloroplasts were distinguished by extraction: buffer-soluble, acid-soluble and firmly bound.
- Intervention effect Extensive washing reduced buffer-soluble ATP in chloroplasts.
- Intervention effect Extensive washing did not reduce acid-soluble or firmly bound ATP.
- Observation Buffer-soluble labeled ATP was almost exclusively gamma labeled.
- Observation Acid-soluble and firmly bound ATP were equally labeled in beta and gamma positions.
- Intervention effect CCCP, desaspidin and phlorizin did not inhibit labeling of firmly bound ATP.
- Intervention effect CCCP, desaspidin and phlorizin almost abolished phosphorylation.
- Observation Postillumination ATP synthesis in chromatophores was inhibited by ATP added after illumination when phosphate was included only in dark incubation.
- Observation Postillumination ATP synthesis in chromatophores was unaffected by ATP added after illumination when phosphate was present only during illumination.
- Intervention effect ADP added after illumination inhibited postillumination ATP formation in chromatophores and chloroplasts only when phosphate was present during illumination.
- Mechanism The authors interpret the data with a two-site coupling factor in which firmly bound ATP drives synthesis of acid-soluble ATP incorporating free phosphate.