Claim · model-written summary
Embryo sensitivity, donor growth conditions, explant type and cocultivation medium most influenced transformation.
- Organ / stage
- immature sorghum embryose0
- Interactor
- Agrobacterium
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Agrobacteriume0other - Outcome
- transformatione0
SorghumNot reportedMaterialGeneCompoundSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
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Verbatim
e0“The four factors that we found most influenced transformation were: the sensitivity of immature sorghum embryos to Agrobacterium infection, the growth conditions of the donor plant, type of explant and co-cultivation medium.”
e1“transformation of sorghum”
Title
Agrobacterium-mediated transformation of sorghum: factors that affect transformation efficiency
Carlos Henrique S. Carvalho; Usha B. Zehr; Nilupa Gunaratna; et al. Genetics and Molecular Biology 2004. doi:10.1590/s1415-47572004000200022
Other claims from this paper (7)
- Method / resource Agrobacterium mediated sorghum transformation was feasible.
- Observation Explants developed necrosis after cocultivation.
- Association Immature embryos were sensitive to Agrobacterium infection, and embryo death limited transformation efficiency.
- Intervention effect Coconut water, vigorous immature embryos and removing excess bacteria significantly improved embryo survival.
- Method / resource Hygromycin phosphotransferase was a good selectable marker.
- Observation GUS activity was low in most transgenic tissues and very high in immature inflorescences.
- Method / resource Overall transformation efficiency remained low.