Claim · model-written summary
Coconut water, vigorous immature embryos and removing excess bacteria significantly improved embryo survival.
- Organ / stage
- sorghum embryose0
- Treatment
- The addition of coconut water to the co-cultivation mediume0
- Outcome
- survival rate of sorghum embryose0
- Direction
- improvede0
SorghumNot reportedMaterialGeneCompoundInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The addition of coconut water to the co-cultivation medium, the use of vigorous and actively growing immature embryos and the removal of excess bacteria significantly improved the survival rate of sorghum embryos”
e1“transformation of sorghum”
Title
Agrobacterium-mediated transformation of sorghum: factors that affect transformation efficiency
Carlos Henrique S. Carvalho; Usha B. Zehr; Nilupa Gunaratna; et al. Genetics and Molecular Biology 2004. doi:10.1590/s1415-47572004000200022
Other claims from this paper (7)
- Method / resource Agrobacterium mediated sorghum transformation was feasible.
- Association Embryo sensitivity, donor growth conditions, explant type and cocultivation medium most influenced transformation.
- Observation Explants developed necrosis after cocultivation.
- Association Immature embryos were sensitive to Agrobacterium infection, and embryo death limited transformation efficiency.
- Method / resource Hygromycin phosphotransferase was a good selectable marker.
- Observation GUS activity was low in most transgenic tissues and very high in immature inflorescences.
- Method / resource Overall transformation efficiency remained low.