Claim · model-written summary
PKABA1 is suggested to act upstream of functional GAMyb formation but downstream of the Slender gene product.
- Taxon
- barley
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Hordeum vulgaree0 - Gene
- PKABA1e7
- Outcome
- formation of functional GAMybe7
Not reportedMaterialCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
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Verbatim
e0“In barley aleurone layers”
e7“we suggest that PKABA1 acts upstream from the formation of functional GAMyb but downstream from the site of action of the Slender gene product”
Title
Aurelio Gómez-Cadenas; Rodolfo Zentella; Mary Kay Walker-Simmons; et al. The Plant Cell 2001. doi:10.1105/tpc.13.3.667
Other claims from this paper (8)
- Intervention effect GAMyb transactivates alpha-amylase expression in barley aleurone cells.
- Intervention effect A truncated GAMyb lacking the transactivation domain prevents GA induction of alpha-amylase.
- Intervention effect ABA and PKABA1 strongly inhibit GA induction of alpha-amylase in barley aleurone cells.
- Intervention effect ABA and PKABA1 have no effect on GAMyb-transactivated alpha-amylase expression.
- Intervention effect ABA and PKABA1 repress GA induction of GAMyb in a GAMyb promoter-beta-glucuronidase construct.
- Observation In the slender mutant, GAMyb and alpha-amylase are highly expressed even without GA.
- Intervention effect Constitutive expression in the slender mutant can still be inhibited by ABA, PKABA1 or a cGMP synthesis inhibitor.
- Mechanism PKABA1 appears to downregulate GAMyb at the transcriptional level.